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Capsaicin receptor immunoreactivity in the human trigeminal ganglion.

Hou, Mingyan; Uddman, Rolf LU ; Tajti, Janos; Kanje, Martin and Edvinsson, Lars LU (2002) In Neuroscience Letters 330(3). p.223-226
Abstract
The cloned capsaicin receptor, also known as vanilloid receptor subtype 1 (VR1) receptor, has been demonstrated to be an integral membrane protein with homology to a family of putative store-operated calcium channels. The VR1 receptor is activated not only by capsaicin but also by noxious heat and protons, and therefore it is suggested as a molecular integrator of chemical and physical stimuli that elicit pain. In the present study, indirect immunofluorescence detected a small number of neurons that are VR1 receptor immunoreactive (ir) (171 versus 1038 or 16% of all neuronal cell bodies) in the human trigeminal ganglion (TG). In addition, RT-PCR confirmed the presence of VR1 mRNA in the human TG. It has been hypothesized that TG neuronal... (More)
The cloned capsaicin receptor, also known as vanilloid receptor subtype 1 (VR1) receptor, has been demonstrated to be an integral membrane protein with homology to a family of putative store-operated calcium channels. The VR1 receptor is activated not only by capsaicin but also by noxious heat and protons, and therefore it is suggested as a molecular integrator of chemical and physical stimuli that elicit pain. In the present study, indirect immunofluorescence detected a small number of neurons that are VR1 receptor immunoreactive (ir) (171 versus 1038 or 16% of all neuronal cell bodies) in the human trigeminal ganglion (TG). In addition, RT-PCR confirmed the presence of VR1 mRNA in the human TG. It has been hypothesized that TG neuronal cell bodies are the source of capsaicin-stimulated release of calcitonin gene-related peptide (CGRP), and hence co-localization experiments were performed. Around 10% of the VR1 receptor-ir is expressed on neurons that contain CGRP-ir (ten among 74) in the human TG, indicating that capsaicin may act through the VR1 receptor to modulate the release of CGRP and in turn to modulate pain. We observed that 8% of the VR1 receptor-ir neuronal cell bodies contain substance P-ir and 5% nitric oxide synthase. Capsaicin can release nitric oxide, CGRP and substance P from sensory nerves and contribute to central sensitization. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Neuroscience Letters
volume
330
issue
3
pages
223 - 226
publisher
Elsevier
external identifiers
  • wos:000179010800003
  • pmid:12270633
  • scopus:0037183781
ISSN
0304-3940
DOI
10.1016/S0304-3940(02)00741-3
language
English
LU publication?
yes
id
9de0a5df-1aa3-4504-8458-252c66244bf3 (old id 110208)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12270633&dopt=Abstract
date added to LUP
2007-06-29 11:10:23
date last changed
2017-08-13 03:30:35
@article{9de0a5df-1aa3-4504-8458-252c66244bf3,
  abstract     = {The cloned capsaicin receptor, also known as vanilloid receptor subtype 1 (VR1) receptor, has been demonstrated to be an integral membrane protein with homology to a family of putative store-operated calcium channels. The VR1 receptor is activated not only by capsaicin but also by noxious heat and protons, and therefore it is suggested as a molecular integrator of chemical and physical stimuli that elicit pain. In the present study, indirect immunofluorescence detected a small number of neurons that are VR1 receptor immunoreactive (ir) (171 versus 1038 or 16% of all neuronal cell bodies) in the human trigeminal ganglion (TG). In addition, RT-PCR confirmed the presence of VR1 mRNA in the human TG. It has been hypothesized that TG neuronal cell bodies are the source of capsaicin-stimulated release of calcitonin gene-related peptide (CGRP), and hence co-localization experiments were performed. Around 10% of the VR1 receptor-ir is expressed on neurons that contain CGRP-ir (ten among 74) in the human TG, indicating that capsaicin may act through the VR1 receptor to modulate the release of CGRP and in turn to modulate pain. We observed that 8% of the VR1 receptor-ir neuronal cell bodies contain substance P-ir and 5% nitric oxide synthase. Capsaicin can release nitric oxide, CGRP and substance P from sensory nerves and contribute to central sensitization.},
  author       = {Hou, Mingyan and Uddman, Rolf and Tajti, Janos and Kanje, Martin and Edvinsson, Lars},
  issn         = {0304-3940},
  language     = {eng},
  number       = {3},
  pages        = {223--226},
  publisher    = {Elsevier},
  series       = {Neuroscience Letters},
  title        = {Capsaicin receptor immunoreactivity in the human trigeminal ganglion.},
  url          = {http://dx.doi.org/10.1016/S0304-3940(02)00741-3},
  volume       = {330},
  year         = {2002},
}