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Methods for the determination in the nanomole range of lipids in liver fine-needle aspiration biopsies

Belfrage, Per LU ; Wiebe, Thomas LU and Lundquist, A (1970) In Scandinavian Journal of Clinical & Laboratory Investigation 26(1). p.53-60
Abstract
Triglycerides and phospholipids 0.5-2mg (wet weight) of human liver material obtained by the fine-needle aspiration biopsy technique can be estimated after chloroform:methanol extraction by the determination of lipid phosphorus and glyceride glycerol. The methods are based on the colorimetric determination of inorganic phosphate after oxidation of the phospholipids and the enzymatic fluorometric determination of glycerol after complete hydrolysis of the triglycerides, catalyzed by purified pancreatic lipase. The accumulation of lipids is then expressed as the increase in the molar ratio of triglycerides over phospholipids.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Aspiration, biopsy, human, liver, phospholipids, ratio TG/PL, triglycerides
in
Scandinavian Journal of Clinical & Laboratory Investigation
volume
26
issue
1
pages
53 - 60
publisher
Informa Healthcare
external identifiers
  • pmid:5457119
  • scopus:0014831702
ISSN
1502-7686
DOI
10.3109/00365517009049214
language
English
LU publication?
yes
id
6cb678e4-0bdb-4e26-a671-37315582717e (old id 1102552)
date added to LUP
2008-08-14 14:08:18
date last changed
2017-01-01 07:26:24
@article{6cb678e4-0bdb-4e26-a671-37315582717e,
  abstract     = {Triglycerides and phospholipids 0.5-2mg (wet weight) of human liver material obtained by the fine-needle aspiration biopsy technique can be estimated after chloroform:methanol extraction by the determination of lipid phosphorus and glyceride glycerol. The methods are based on the colorimetric determination of inorganic phosphate after oxidation of the phospholipids and the enzymatic fluorometric determination of glycerol after complete hydrolysis of the triglycerides, catalyzed by purified pancreatic lipase. The accumulation of lipids is then expressed as the increase in the molar ratio of triglycerides over phospholipids.},
  author       = {Belfrage, Per and Wiebe, Thomas and Lundquist, A},
  issn         = {1502-7686},
  keyword      = {Aspiration,biopsy,human,liver,phospholipids,ratio TG/PL,triglycerides},
  language     = {eng},
  number       = {1},
  pages        = {53--60},
  publisher    = {Informa Healthcare},
  series       = {Scandinavian Journal of Clinical & Laboratory Investigation},
  title        = {Methods for the determination in the nanomole range of lipids in liver fine-needle aspiration biopsies},
  url          = {http://dx.doi.org/10.3109/00365517009049214},
  volume       = {26},
  year         = {1970},
}