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Time-course of liver fat accumulation in man after a single load of ethanol

Wiebe, Thomas LU ; Lundquist, A and Belfrage, Per LU (1971) In Scandinavian Journal of Clinical & Laboratory Investigation 27(1). p.33-36
Abstract
Three healthy young male subjects were given a single load of ethanol (approximately 3 g/kg body weight) over 5 hours. Liver aspiration biopsies were performed at the start of the experiment and 6, 14, 24, and 48 hours after the start. Blood alcohol and serum transaminase activity levels were measured simultaneously. Triglycerides and phospholipids of the liver biopsy material were determined by a previously described method. The increase of the molar ratio triglycerides over phospholipids (TG/PL) was used to express triglyceride accumulation in the livers. At 24 hours from the start of the experiment this ratio had increased 2-5 times, and then declined to almost the initial values at 48 hours. Serum transaminases showed a slight increase... (More)
Three healthy young male subjects were given a single load of ethanol (approximately 3 g/kg body weight) over 5 hours. Liver aspiration biopsies were performed at the start of the experiment and 6, 14, 24, and 48 hours after the start. Blood alcohol and serum transaminase activity levels were measured simultaneously. Triglycerides and phospholipids of the liver biopsy material were determined by a previously described method. The increase of the molar ratio triglycerides over phospholipids (TG/PL) was used to express triglyceride accumulation in the livers. At 24 hours from the start of the experiment this ratio had increased 2-5 times, and then declined to almost the initial values at 48 hours. Serum transaminases showed a slight increase over the entire experimental period. Considerable individual variation in the parameters was found. The results show that it is possible to follow time-course of rapid metabolic changes in the human liver by a combination of the fine-needle aspiration biopsy technique and biochemical micro-methods. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Accumulation, aspiration, biopsy, ethanol, fat, human, ingestion, liver, time-course
in
Scandinavian Journal of Clinical & Laboratory Investigation
volume
27
issue
1
pages
33 - 36
publisher
Informa Healthcare
external identifiers
  • pmid:5100060
  • scopus:0015015567
ISSN
1502-7686
DOI
10.3109/00365517109080186
language
English
LU publication?
yes
id
04b795ca-593f-4399-a019-3e074ef0ec2c (old id 1102557)
date added to LUP
2008-08-14 14:05:59
date last changed
2017-01-01 07:09:45
@article{04b795ca-593f-4399-a019-3e074ef0ec2c,
  abstract     = {Three healthy young male subjects were given a single load of ethanol (approximately 3 g/kg body weight) over 5 hours. Liver aspiration biopsies were performed at the start of the experiment and 6, 14, 24, and 48 hours after the start. Blood alcohol and serum transaminase activity levels were measured simultaneously. Triglycerides and phospholipids of the liver biopsy material were determined by a previously described method. The increase of the molar ratio triglycerides over phospholipids (TG/PL) was used to express triglyceride accumulation in the livers. At 24 hours from the start of the experiment this ratio had increased 2-5 times, and then declined to almost the initial values at 48 hours. Serum transaminases showed a slight increase over the entire experimental period. Considerable individual variation in the parameters was found. The results show that it is possible to follow time-course of rapid metabolic changes in the human liver by a combination of the fine-needle aspiration biopsy technique and biochemical micro-methods.},
  author       = {Wiebe, Thomas and Lundquist, A and Belfrage, Per},
  issn         = {1502-7686},
  keyword      = {Accumulation,aspiration,biopsy,ethanol,fat,human,ingestion,liver,time-course},
  language     = {eng},
  number       = {1},
  pages        = {33--36},
  publisher    = {Informa Healthcare},
  series       = {Scandinavian Journal of Clinical & Laboratory Investigation},
  title        = {Time-course of liver fat accumulation in man after a single load of ethanol},
  url          = {http://dx.doi.org/10.3109/00365517109080186},
  volume       = {27},
  year         = {1971},
}