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Dispersion of viable pig liver cells with collagenase

Belfrage, Per LU ; Börjesson, Bengt; Hägerstrand, Inga; Nilsson, Åke LU ; Olsson, Arne; Wiebe, Thomas LU and Akesson, Björn (1975) In Life Sciences 17(8). p.1219-1225
Abstract
Viable suspended hepatocytes were prepared from surgical biopsy specimens of pig and human liver by digestion with collagenase. Initial perfusion of the tissue through cannulated blood vessels with 0.5 mM EGTA followed by 0.2% collagenase gave the best results. 20−870 × 106 cells of which 60–95 % excluded trypan blue were obtained from 5–30 g pig liver pieces, while results with human liver specimens were usually less satisfactory. In some experiments, however, viable cells, as judged by vital stain exclusion and ability to synthesize lipids were obtained in sufficient yield. In the pig hepatocytes glycerolipid synthesis from [3H] glycerol and oxidation and esterification of [14C] oleic acid had the same characteristics as those observed... (More)
Viable suspended hepatocytes were prepared from surgical biopsy specimens of pig and human liver by digestion with collagenase. Initial perfusion of the tissue through cannulated blood vessels with 0.5 mM EGTA followed by 0.2% collagenase gave the best results. 20−870 × 106 cells of which 60–95 % excluded trypan blue were obtained from 5–30 g pig liver pieces, while results with human liver specimens were usually less satisfactory. In some experiments, however, viable cells, as judged by vital stain exclusion and ability to synthesize lipids were obtained in sufficient yield. In the pig hepatocytes glycerolipid synthesis from [3H] glycerol and oxidation and esterification of [14C] oleic acid had the same characteristics as those observed earlier in rat hepatocytes. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Life Sciences
volume
17
issue
8
pages
1219 - 1225
publisher
Elsevier
external identifiers
  • pmid:172754
  • scopus:0016803010
ISSN
1879-0631
DOI
10.1016/0024-3205(75)90130-7
language
English
LU publication?
yes
id
c28dcb10-d200-4cf5-aad0-ba80ce33ab61 (old id 1102592)
date added to LUP
2008-08-14 13:02:59
date last changed
2017-01-01 05:07:25
@article{c28dcb10-d200-4cf5-aad0-ba80ce33ab61,
  abstract     = {Viable suspended hepatocytes were prepared from surgical biopsy specimens of pig and human liver by digestion with collagenase. Initial perfusion of the tissue through cannulated blood vessels with 0.5 mM EGTA followed by 0.2% collagenase gave the best results. 20−870 × 106 cells of which 60–95 % excluded trypan blue were obtained from 5–30 g pig liver pieces, while results with human liver specimens were usually less satisfactory. In some experiments, however, viable cells, as judged by vital stain exclusion and ability to synthesize lipids were obtained in sufficient yield. In the pig hepatocytes glycerolipid synthesis from [3H] glycerol and oxidation and esterification of [14C] oleic acid had the same characteristics as those observed earlier in rat hepatocytes.},
  author       = {Belfrage, Per and Börjesson, Bengt and Hägerstrand, Inga and Nilsson, Åke and Olsson, Arne and Wiebe, Thomas and Akesson, Björn},
  issn         = {1879-0631},
  language     = {eng},
  number       = {8},
  pages        = {1219--1225},
  publisher    = {Elsevier},
  series       = {Life Sciences},
  title        = {Dispersion of viable pig liver cells with collagenase},
  url          = {http://dx.doi.org/10.1016/0024-3205(75)90130-7},
  volume       = {17},
  year         = {1975},
}