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A stable, radioactive substrate emulsion for assay of lipoprotein lipase

Nilsson-Ehle, Peter LU and Schotz, Michael C (1976) In Journal of Lipid Research 17(5). p.536-541
Abstract
A method is described for the assay of lipoprotein lipase, using a stable, radioactive substrate emulsion. Fatty acid-labeled trioleoylglycerol was emulsified by homogenization in glycerol with lecithin as detergent. This anhydrous emulsion was stable for at least six weeks. Substrate solutions for enzyme assay were prepared by diluting the emulsion with buffer containing serum and albumin. The fatty acid produced on hydrolysis was isolated in a one-step liquid-liquid partition system. Incubations with extracts of acetone powders from adipose tissue displayed characteristics of lipoprotein lipase activity, i.e., serum dependence and inhibition by NaCl and protamine. The method is rapid (less than 1 hour), sensitive and reproducible, and... (More)
A method is described for the assay of lipoprotein lipase, using a stable, radioactive substrate emulsion. Fatty acid-labeled trioleoylglycerol was emulsified by homogenization in glycerol with lecithin as detergent. This anhydrous emulsion was stable for at least six weeks. Substrate solutions for enzyme assay were prepared by diluting the emulsion with buffer containing serum and albumin. The fatty acid produced on hydrolysis was isolated in a one-step liquid-liquid partition system. Incubations with extracts of acetone powders from adipose tissue displayed characteristics of lipoprotein lipase activity, i.e., serum dependence and inhibition by NaCl and protamine. The method is rapid (less than 1 hour), sensitive and reproducible, and suitable for routine use. (Less)
Please use this url to cite or link to this publication:
author
publishing date
type
Contribution to journal
publication status
published
subject
keywords
adipose lipoprotein lipase tri[3H]oleoylglycerol, triglyceride, postheparin serum
in
Journal of Lipid Research
volume
17
issue
5
pages
536 - 541
publisher
American Society for Biochemistry and Molecular Biology
external identifiers
  • pmid:9464
  • scopus:0017202148
ISSN
1539-7262
language
English
LU publication?
no
id
e2b82209-7f14-4d86-8583-d1214f49eab2 (old id 1102620)
alternative location
http://www.jlr.org/cgi/reprint/17/5/536
date added to LUP
2008-08-14 11:42:35
date last changed
2017-08-20 03:31:18
@article{e2b82209-7f14-4d86-8583-d1214f49eab2,
  abstract     = {A method is described for the assay of lipoprotein lipase, using a stable, radioactive substrate emulsion. Fatty acid-labeled trioleoylglycerol was emulsified by homogenization in glycerol with lecithin as detergent. This anhydrous emulsion was stable for at least six weeks. Substrate solutions for enzyme assay were prepared by diluting the emulsion with buffer containing serum and albumin. The fatty acid produced on hydrolysis was isolated in a one-step liquid-liquid partition system. Incubations with extracts of acetone powders from adipose tissue displayed characteristics of lipoprotein lipase activity, i.e., serum dependence and inhibition by NaCl and protamine. The method is rapid (less than 1 hour), sensitive and reproducible, and suitable for routine use.},
  author       = {Nilsson-Ehle, Peter and Schotz, Michael C},
  issn         = {1539-7262},
  keyword      = {adipose lipoprotein lipase tri[3H]oleoylglycerol,triglyceride,postheparin serum},
  language     = {eng},
  number       = {5},
  pages        = {536--541},
  publisher    = {American Society for Biochemistry and Molecular Biology},
  series       = {Journal of Lipid Research},
  title        = {A stable, radioactive substrate emulsion for assay of lipoprotein lipase},
  volume       = {17},
  year         = {1976},
}