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No in vivo effect of trisodium phosphonoformate on woodchuck hepatitis virus production

Nordenfelt, E; Widell, Anders LU ; Hansson, B G; Löfgren, B; Möller-Nielsen, C and Oberg, B (1982) In Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology 90(6). p.449-451
Abstract
The efficient in vitro inhibition of hepatitis B virus DNA polymerase by trisodium phosphonoformate (PFA, INN: foscarnet sodium) and its low toxicity suggested that PFA could be used as a therapeutic agent for hepatitis B infection. PFA was also found to inhibit woodchuck hepatitis virus (WHV) DNA polymerase in vitro. As a model to test PFA's eventual effect, chronically WHV infected woodchucks were treated with PFA. The animals were treated twice daily in a dosage which gave a minimum serum level of PFA corresponding to an in vitro inhibiting effect on WHV DNA polymerase of about 40%. The concentration in liver tissue was found to be 15% below serum level. The amount of WHV particles in serum was followed by DNA polymerase assay. No... (More)
The efficient in vitro inhibition of hepatitis B virus DNA polymerase by trisodium phosphonoformate (PFA, INN: foscarnet sodium) and its low toxicity suggested that PFA could be used as a therapeutic agent for hepatitis B infection. PFA was also found to inhibit woodchuck hepatitis virus (WHV) DNA polymerase in vitro. As a model to test PFA's eventual effect, chronically WHV infected woodchucks were treated with PFA. The animals were treated twice daily in a dosage which gave a minimum serum level of PFA corresponding to an in vitro inhibiting effect on WHV DNA polymerase of about 40%. The concentration in liver tissue was found to be 15% below serum level. The amount of WHV particles in serum was followed by DNA polymerase assay. No effect on WHV production could be seen during 2 weeks' treatment. No change of the in vitro sensitivity to PFA of the WHV DNA polymerase was seen. These results indicate that the WHV associated DNA polymerase has no role in the production of viral particles. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology
volume
90
issue
6
pages
449 - 451
publisher
Munksgaard International Publishers
external identifiers
  • pmid:6220563
  • scopus:0020362976
ISSN
0108-0180
language
English
LU publication?
yes
id
3f48ca14-c3e7-4a1f-aeb7-2ed7416e2332 (old id 1102894)
date added to LUP
2008-08-13 13:23:40
date last changed
2017-08-06 04:27:01
@article{3f48ca14-c3e7-4a1f-aeb7-2ed7416e2332,
  abstract     = {The efficient in vitro inhibition of hepatitis B virus DNA polymerase by trisodium phosphonoformate (PFA, INN: foscarnet sodium) and its low toxicity suggested that PFA could be used as a therapeutic agent for hepatitis B infection. PFA was also found to inhibit woodchuck hepatitis virus (WHV) DNA polymerase in vitro. As a model to test PFA's eventual effect, chronically WHV infected woodchucks were treated with PFA. The animals were treated twice daily in a dosage which gave a minimum serum level of PFA corresponding to an in vitro inhibiting effect on WHV DNA polymerase of about 40%. The concentration in liver tissue was found to be 15% below serum level. The amount of WHV particles in serum was followed by DNA polymerase assay. No effect on WHV production could be seen during 2 weeks' treatment. No change of the in vitro sensitivity to PFA of the WHV DNA polymerase was seen. These results indicate that the WHV associated DNA polymerase has no role in the production of viral particles.},
  author       = {Nordenfelt, E and Widell, Anders and Hansson, B G and Löfgren, B and Möller-Nielsen, C and Oberg, B},
  issn         = {0108-0180},
  language     = {eng},
  number       = {6},
  pages        = {449--451},
  publisher    = {Munksgaard International Publishers},
  series       = {Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology},
  title        = {No in vivo effect of trisodium phosphonoformate on woodchuck hepatitis virus production},
  volume       = {90},
  year         = {1982},
}