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Quantitative analysis of rat Ig (sub) classes binding to cell surface antigens

Nilsson, Rune LU ; Brodin, Thomas and Sjögren, Hans Olov LU (1982) In Journal of Immunological Methods 55(2). p.179-191
Abstract
An indirect 125I-labeled protein A assay for detection of cell surface-bound rat immunoglobulins is presented. The assay is quantitative and rapid and detects as little as 1 ng of cell surface-bound Ig. It discriminates between antibodies belonging to different IgG subclasses, IgM and IgA. We describe the production and specificity control of the reagents used and show that the test can be used for quantitative analysis. A large number of sera from untreated rats are tested to evaluate the frequency of falsely positive responses and variation due to age, sex and strain of rat. With this test it is relatively easy to quantitate the binding of classes and subclasses of rat immunoglobulins in a small volume (6 μl) of untreated serum.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
protein A, cell surface antigens, rat Ig (sub)classes, binding test, monoclonal Ig
in
Journal of Immunological Methods
volume
55
issue
2
pages
179 - 191
publisher
Elsevier
external identifiers
  • pmid:6186740
  • scopus:0020356093
ISSN
1872-7905
DOI
10.1016/0022-1759(82)90030-8
language
English
LU publication?
yes
id
131d7354-07eb-49b7-9a5d-9ba19b4cea42 (old id 1102942)
date added to LUP
2008-08-13 13:57:43
date last changed
2017-02-26 04:20:38
@article{131d7354-07eb-49b7-9a5d-9ba19b4cea42,
  abstract     = {An indirect 125I-labeled protein A assay for detection of cell surface-bound rat immunoglobulins is presented. The assay is quantitative and rapid and detects as little as 1 ng of cell surface-bound Ig. It discriminates between antibodies belonging to different IgG subclasses, IgM and IgA. We describe the production and specificity control of the reagents used and show that the test can be used for quantitative analysis. A large number of sera from untreated rats are tested to evaluate the frequency of falsely positive responses and variation due to age, sex and strain of rat. With this test it is relatively easy to quantitate the binding of classes and subclasses of rat immunoglobulins in a small volume (6 μl) of untreated serum.},
  author       = {Nilsson, Rune and Brodin, Thomas and Sjögren, Hans Olov},
  issn         = {1872-7905},
  keyword      = {protein A,cell surface antigens,rat Ig (sub)classes,binding test,monoclonal Ig},
  language     = {eng},
  number       = {2},
  pages        = {179--191},
  publisher    = {Elsevier},
  series       = {Journal of Immunological Methods},
  title        = {Quantitative analysis of rat Ig (sub) classes binding to cell surface antigens},
  url          = {http://dx.doi.org/10.1016/0022-1759(82)90030-8},
  volume       = {55},
  year         = {1982},
}