Quantitative analysis of rat Ig (sub) classes binding to cell surface antigens
(1982) In Journal of Immunological Methods 55(2). p.179-191- Abstract
- An indirect 125I-labeled protein A assay for detection of cell surface-bound rat immunoglobulins is presented. The assay is quantitative and rapid and detects as little as 1 ng of cell surface-bound Ig. It discriminates between antibodies belonging to different IgG subclasses, IgM and IgA. We describe the production and specificity control of the reagents used and show that the test can be used for quantitative analysis. A large number of sera from untreated rats are tested to evaluate the frequency of falsely positive responses and variation due to age, sex and strain of rat. With this test it is relatively easy to quantitate the binding of classes and subclasses of rat immunoglobulins in a small volume (6 μl) of untreated serum.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1102942
- author
- Nilsson, Rune LU ; Brodin, Thomas and Sjögren, Hans Olov LU
- organization
- publishing date
- 1982
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- protein A, cell surface antigens, rat Ig (sub)classes, binding test, monoclonal Ig
- in
- Journal of Immunological Methods
- volume
- 55
- issue
- 2
- pages
- 179 - 191
- publisher
- Elsevier
- external identifiers
-
- pmid:6186740
- scopus:0020356093
- ISSN
- 1872-7905
- DOI
- 10.1016/0022-1759(82)90030-8
- language
- English
- LU publication?
- yes
- id
- 131d7354-07eb-49b7-9a5d-9ba19b4cea42 (old id 1102942)
- date added to LUP
- 2016-04-01 17:12:16
- date last changed
- 2021-01-03 08:43:47
@article{131d7354-07eb-49b7-9a5d-9ba19b4cea42, abstract = {{An indirect 125I-labeled protein A assay for detection of cell surface-bound rat immunoglobulins is presented. The assay is quantitative and rapid and detects as little as 1 ng of cell surface-bound Ig. It discriminates between antibodies belonging to different IgG subclasses, IgM and IgA. We describe the production and specificity control of the reagents used and show that the test can be used for quantitative analysis. A large number of sera from untreated rats are tested to evaluate the frequency of falsely positive responses and variation due to age, sex and strain of rat. With this test it is relatively easy to quantitate the binding of classes and subclasses of rat immunoglobulins in a small volume (6 μl) of untreated serum.}}, author = {{Nilsson, Rune and Brodin, Thomas and Sjögren, Hans Olov}}, issn = {{1872-7905}}, keywords = {{protein A; cell surface antigens; rat Ig (sub)classes; binding test; monoclonal Ig}}, language = {{eng}}, number = {{2}}, pages = {{179--191}}, publisher = {{Elsevier}}, series = {{Journal of Immunological Methods}}, title = {{Quantitative analysis of rat Ig (sub) classes binding to cell surface antigens}}, url = {{http://dx.doi.org/10.1016/0022-1759(82)90030-8}}, doi = {{10.1016/0022-1759(82)90030-8}}, volume = {{55}}, year = {{1982}}, }