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Different protein A immunosorbents may have different binding specificity for rat immunoglobulins

Nilsson, Rune LU ; Myhre, E; Kronvall, G and Sjögren, H O (1983) In Journal of Immunological Methods 62(2). p.241-245
Abstract
Purified polyclonal immunoglobulin preparations representing the 4 rat IgG subclasses were tested for binding to Staphylococcus aureus protein A attached to 3 different solid phases (Staphylococcus aureus Cowan I bacteria, Sepharose CL4B and Sepharose 6MB). Protein A Sepharose CL4B showed higher reactivity with IgG1 and IgG2b than the staphylococci, whereas protein A Sepharose 6MB showed a lower uptake of these subclasses. No differences were seen for IgG2a and IgG2c. Protein A on different solid phases cannot be used interchangeably without confirmation of binding specificity.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
protein A-Sepharose, protein A macrobeads, rat IgG subclasses, immunosorbents
in
Journal of Immunological Methods
volume
62
issue
2
pages
241 - 245
publisher
Elsevier
external identifiers
  • pmid:6350460
  • scopus:0020511158
ISSN
1872-7905
DOI
10.1016/0022-1759(83)90251-X
language
English
LU publication?
yes
id
2ff95b87-5036-49b9-90b7-d6e8f383acef (old id 1103043)
date added to LUP
2008-08-13 10:58:48
date last changed
2017-07-30 04:42:58
@article{2ff95b87-5036-49b9-90b7-d6e8f383acef,
  abstract     = {Purified polyclonal immunoglobulin preparations representing the 4 rat IgG subclasses were tested for binding to Staphylococcus aureus protein A attached to 3 different solid phases (Staphylococcus aureus Cowan I bacteria, Sepharose CL4B and Sepharose 6MB). Protein A Sepharose CL4B showed higher reactivity with IgG1 and IgG2b than the staphylococci, whereas protein A Sepharose 6MB showed a lower uptake of these subclasses. No differences were seen for IgG2a and IgG2c. Protein A on different solid phases cannot be used interchangeably without confirmation of binding specificity.},
  author       = {Nilsson, Rune and Myhre, E and Kronvall, G and Sjögren, H O},
  issn         = {1872-7905},
  keyword      = {protein A-Sepharose,protein A macrobeads,rat IgG subclasses,immunosorbents},
  language     = {eng},
  number       = {2},
  pages        = {241--245},
  publisher    = {Elsevier},
  series       = {Journal of Immunological Methods},
  title        = {Different protein A immunosorbents may have different binding specificity for rat immunoglobulins},
  url          = {http://dx.doi.org/10.1016/0022-1759(83)90251-X},
  volume       = {62},
  year         = {1983},
}