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Surface characteristics of group A streptococci with and without M-protein

Miörner, Håkan LU ; Havlicek, J and Kronvall, G (1984) In Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology 92(1). p.23-30
Abstract
Twenty M protein-positive and eight M protein-negative strains of group A streptococci were investigated with respect to surface hydrophobicity and amount of lipoteichoic acid (LTA). Surface hydrophobicity as determined in polymer two-phase systems varied substantially between individual strains and there was no correlation to the presence of antiphagocytic M protein. The amount of LTA on the surface of the bacteria varied with hydrophobic affinity of the cells. Strains with a high content of surface LTA were found among both M-positive and M-negative streptococci. Cellular and extracellular LTA was estimated on six strains by the ability of hot phenol extracts and culture fluids to sensitize erythrocytes and by rocket... (More)
Twenty M protein-positive and eight M protein-negative strains of group A streptococci were investigated with respect to surface hydrophobicity and amount of lipoteichoic acid (LTA). Surface hydrophobicity as determined in polymer two-phase systems varied substantially between individual strains and there was no correlation to the presence of antiphagocytic M protein. The amount of LTA on the surface of the bacteria varied with hydrophobic affinity of the cells. Strains with a high content of surface LTA were found among both M-positive and M-negative streptococci. Cellular and extracellular LTA was estimated on six strains by the ability of hot phenol extracts and culture fluids to sensitize erythrocytes and by rocket immunoelectrophoretic quantitation. Differences in content of surface LTA did not correlate to differences in the total amount of cellular LTA. Pepsin digestion of M-positive group A streptococci at suboptimal pH resulted in a loss of M antigen whereas surface LTA and the hydrophobic interaction liability was retained. The results indicate that the degree of surface hydrophobicity as measured by two-phase partitioning is not correlated to either the type-specific or the antiphagocytic moiety of M protein. The results support the correlation between surface LTA and surface hydrophobicity of group A streptococci. (Less)
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organization
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type
Contribution to journal
publication status
published
subject
in
Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology
volume
92
issue
1
pages
23 - 30
publisher
Munksgaard International Publishers
external identifiers
  • pmid:6424406
  • scopus:0021358356
ISSN
0108-0180
language
English
LU publication?
yes
id
2f051c0f-0267-4f2c-b72e-9c1b8cbc9acf (old id 1103253)
date added to LUP
2008-08-12 15:02:25
date last changed
2017-09-03 04:42:15
@article{2f051c0f-0267-4f2c-b72e-9c1b8cbc9acf,
  abstract     = {Twenty M protein-positive and eight M protein-negative strains of group A streptococci were investigated with respect to surface hydrophobicity and amount of lipoteichoic acid (LTA). Surface hydrophobicity as determined in polymer two-phase systems varied substantially between individual strains and there was no correlation to the presence of antiphagocytic M protein. The amount of LTA on the surface of the bacteria varied with hydrophobic affinity of the cells. Strains with a high content of surface LTA were found among both M-positive and M-negative streptococci. Cellular and extracellular LTA was estimated on six strains by the ability of hot phenol extracts and culture fluids to sensitize erythrocytes and by rocket immunoelectrophoretic quantitation. Differences in content of surface LTA did not correlate to differences in the total amount of cellular LTA. Pepsin digestion of M-positive group A streptococci at suboptimal pH resulted in a loss of M antigen whereas surface LTA and the hydrophobic interaction liability was retained. The results indicate that the degree of surface hydrophobicity as measured by two-phase partitioning is not correlated to either the type-specific or the antiphagocytic moiety of M protein. The results support the correlation between surface LTA and surface hydrophobicity of group A streptococci.},
  author       = {Miörner, Håkan and Havlicek, J and Kronvall, G},
  issn         = {0108-0180},
  language     = {eng},
  number       = {1},
  pages        = {23--30},
  publisher    = {Munksgaard International Publishers},
  series       = {Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology},
  title        = {Surface characteristics of group A streptococci with and without M-protein},
  volume       = {92},
  year         = {1984},
}