Advanced

Assay of type-specific M antigens on whole group A streptococci

Miörner, Håkan LU and Nivenius Larsson, K (1984) In European journal of clinical microbiology 3(1). p.14-18
Abstract
A novel radioimmunoassay of type-specific M antigens on whole group A streptococcal cells is described. Absorbed rabbit anti-M antisera directed against M types 12 and 49 were used for determining M antigens on intact bacterial organisms. Staphylococcal protein A labelled with 125I was used as an anti-antibody reagent. The absorbed antisera were tested against ten homologous and 48 heterologous serotypes. All homologous serotypes gave an unequivocal reaction distinct from the weaker reaction with the heterologous serotypes. The type-specificity of the reaction was confirmed by the removal of type-specific antibodies after absorption to purified M protein coupled to Sepharose 4B. The results indicate that the described method is a simple... (More)
A novel radioimmunoassay of type-specific M antigens on whole group A streptococcal cells is described. Absorbed rabbit anti-M antisera directed against M types 12 and 49 were used for determining M antigens on intact bacterial organisms. Staphylococcal protein A labelled with 125I was used as an anti-antibody reagent. The absorbed antisera were tested against ten homologous and 48 heterologous serotypes. All homologous serotypes gave an unequivocal reaction distinct from the weaker reaction with the heterologous serotypes. The type-specificity of the reaction was confirmed by the removal of type-specific antibodies after absorption to purified M protein coupled to Sepharose 4B. The results indicate that the described method is a simple and reliable technique for the recognition of M types of group A streptococci and offers a valuable tool for studies of M antigen in situ. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
European journal of clinical microbiology
volume
3
issue
1
pages
14 - 18
publisher
Springer
external identifiers
  • pmid:6368223
  • scopus:0021354133
ISSN
0722-2211
DOI
10.1007/BF02032808
language
English
LU publication?
yes
id
175514e3-e434-43e4-a716-461f69c165c8 (old id 1103256)
date added to LUP
2008-08-12 15:06:47
date last changed
2017-04-21 01:36:05
@article{175514e3-e434-43e4-a716-461f69c165c8,
  abstract     = {A novel radioimmunoassay of type-specific M antigens on whole group A streptococcal cells is described. Absorbed rabbit anti-M antisera directed against M types 12 and 49 were used for determining M antigens on intact bacterial organisms. Staphylococcal protein A labelled with 125I was used as an anti-antibody reagent. The absorbed antisera were tested against ten homologous and 48 heterologous serotypes. All homologous serotypes gave an unequivocal reaction distinct from the weaker reaction with the heterologous serotypes. The type-specificity of the reaction was confirmed by the removal of type-specific antibodies after absorption to purified M protein coupled to Sepharose 4B. The results indicate that the described method is a simple and reliable technique for the recognition of M types of group A streptococci and offers a valuable tool for studies of M antigen in situ.},
  author       = {Miörner, Håkan and Nivenius Larsson, K},
  issn         = {0722-2211},
  language     = {eng},
  number       = {1},
  pages        = {14--18},
  publisher    = {Springer},
  series       = {European journal of clinical microbiology},
  title        = {Assay of type-specific M antigens on whole group A streptococci},
  url          = {http://dx.doi.org/10.1007/BF02032808},
  volume       = {3},
  year         = {1984},
}