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Cell cycle phase-dependent induction of ornithine decarboxylase-antizyme.

Linden, Margareta; Anehus, Siw; Långström-Einarsson, Eva LU ; Baldetorp, Bo LU and Heby, Olle (1985) In Journal of Cellular Physiology 125(2). p.273-276
Abstract
The activites of ornithine decarboxylase (ODC) and ODC inhibitory protein (ODC-antizyme) were studied in Ehrlich ascites tumor cells, separated according to their position in the cell cycle by centrifugal elutriation. Release and/or synthesis of ODC-antizyme was induced by putrescine treatment. Each mouse received an intraperitoneal injection of 25 moles of putrescine at 0, 1, 2, and 3 hr after tumor transplantation. Tumor cells obtained from putrescine-treated and control mice at 4 hr after transplantation were separated into fractions representing all phases of the cell cycle. The cell cycle distribution of the tumor cells in each fraction was determined by flow cytometry.

In control tumor cells the ODC activity exhibited two... (More)
The activites of ornithine decarboxylase (ODC) and ODC inhibitory protein (ODC-antizyme) were studied in Ehrlich ascites tumor cells, separated according to their position in the cell cycle by centrifugal elutriation. Release and/or synthesis of ODC-antizyme was induced by putrescine treatment. Each mouse received an intraperitoneal injection of 25 moles of putrescine at 0, 1, 2, and 3 hr after tumor transplantation. Tumor cells obtained from putrescine-treated and control mice at 4 hr after transplantation were separated into fractions representing all phases of the cell cycle. The cell cycle distribution of the tumor cells in each fraction was determined by flow cytometry.

In control tumor cells the ODC activity exhibited two maxima; inlate-G1/early-S and in late-S/G2. A marked decrease in ODC activity was observed in mid-S phase. This decrease coincided with maximum ODC-antizyme activity (revealed by putrescine treatment), suggesting that ODC-antizyme is involved in the regulation of ODC activity during the cell cycle. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Cellular Physiology
volume
125
issue
2
pages
273 - 276
publisher
John Wiley & Sons
external identifiers
  • scopus:0022381141
ISSN
1097-4652
DOI
10.1002/jcp.1041250215
language
English
LU publication?
yes
id
413aee54-5941-4bc6-9117-2d10d6f2aa1e (old id 1103453)
date added to LUP
2008-08-11 14:16:23
date last changed
2017-08-27 04:18:08
@article{413aee54-5941-4bc6-9117-2d10d6f2aa1e,
  abstract     = {The activites of ornithine decarboxylase (ODC) and ODC inhibitory protein (ODC-antizyme) were studied in Ehrlich ascites tumor cells, separated according to their position in the cell cycle by centrifugal elutriation. Release and/or synthesis of ODC-antizyme was induced by putrescine treatment. Each mouse received an intraperitoneal injection of 25 moles of putrescine at 0, 1, 2, and 3 hr after tumor transplantation. Tumor cells obtained from putrescine-treated and control mice at 4 hr after transplantation were separated into fractions representing all phases of the cell cycle. The cell cycle distribution of the tumor cells in each fraction was determined by flow cytometry.<br/><br>
In control tumor cells the ODC activity exhibited two maxima; inlate-G1/early-S and in late-S/G2. A marked decrease in ODC activity was observed in mid-S phase. This decrease coincided with maximum ODC-antizyme activity (revealed by putrescine treatment), suggesting that ODC-antizyme is involved in the regulation of ODC activity during the cell cycle.},
  author       = {Linden, Margareta and Anehus, Siw and Långström-Einarsson, Eva and Baldetorp, Bo and Heby, Olle},
  issn         = {1097-4652},
  language     = {eng},
  number       = {2},
  pages        = {273--276},
  publisher    = {John Wiley & Sons},
  series       = {Journal of Cellular Physiology},
  title        = {Cell cycle phase-dependent induction of ornithine decarboxylase-antizyme.},
  url          = {http://dx.doi.org/10.1002/jcp.1041250215},
  volume       = {125},
  year         = {1985},
}