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Influence of twenty potentially antiviral substances on in vitro multiplication of hepatitis A virus

Widell, Anders LU ; Hansson, Bengt-Göran LU ; Öberg, Bo and Nordenfelt, Erik (1986) In Antiviral Research 6(2). p.103-112
Abstract
A multiwell tissue culture system was developed to study the influence of various substances on hepatitis A virus (HAV) propagation. A panel of 20 substances of different structure types, each with known effect against at least some viruses, was studied at a concentration of 100 microM. Three substances showed reproducible inhibition. The strongest inhibitor, arabinosylcytosine, also produced cytotoxic changes in cells down to a concentration of 1 microM, and its effect was considered as nonspecific. Amantadine and ribavirin showed a moderate effect at 100 microM. A stronger inhibition was seen at 250 and 500 microM, doses that are toxic and impractical for clinical use. Although no promising candidates for antiviral treatment of hepatitis... (More)
A multiwell tissue culture system was developed to study the influence of various substances on hepatitis A virus (HAV) propagation. A panel of 20 substances of different structure types, each with known effect against at least some viruses, was studied at a concentration of 100 microM. Three substances showed reproducible inhibition. The strongest inhibitor, arabinosylcytosine, also produced cytotoxic changes in cells down to a concentration of 1 microM, and its effect was considered as nonspecific. Amantadine and ribavirin showed a moderate effect at 100 microM. A stronger inhibition was seen at 250 and 500 microM, doses that are toxic and impractical for clinical use. Although no promising candidates for antiviral treatment of hepatitis A have emerged from the present study, the assay model described here would seem useful in the screening of substances with inhibitory effects on HAV. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
ribavirin, amantadine, arabinosylcytosine, hepatitis A
in
Antiviral Research
volume
6
issue
2
pages
103 - 112
publisher
Elsevier
external identifiers
  • pmid:3010855
  • scopus:0022586905
ISSN
0166-3542
DOI
10.1016/0166-3542(86)90030-6
language
English
LU publication?
yes
id
f43ee2d9-f679-4a0e-b6f2-9a64a9da01a2 (old id 1103497)
date added to LUP
2008-08-11 08:32:26
date last changed
2017-07-23 03:44:18
@article{f43ee2d9-f679-4a0e-b6f2-9a64a9da01a2,
  abstract     = {A multiwell tissue culture system was developed to study the influence of various substances on hepatitis A virus (HAV) propagation. A panel of 20 substances of different structure types, each with known effect against at least some viruses, was studied at a concentration of 100 microM. Three substances showed reproducible inhibition. The strongest inhibitor, arabinosylcytosine, also produced cytotoxic changes in cells down to a concentration of 1 microM, and its effect was considered as nonspecific. Amantadine and ribavirin showed a moderate effect at 100 microM. A stronger inhibition was seen at 250 and 500 microM, doses that are toxic and impractical for clinical use. Although no promising candidates for antiviral treatment of hepatitis A have emerged from the present study, the assay model described here would seem useful in the screening of substances with inhibitory effects on HAV.},
  author       = {Widell, Anders and Hansson, Bengt-Göran and Öberg, Bo and Nordenfelt, Erik},
  issn         = {0166-3542},
  keyword      = {ribavirin,amantadine,arabinosylcytosine,hepatitis A},
  language     = {eng},
  number       = {2},
  pages        = {103--112},
  publisher    = {Elsevier},
  series       = {Antiviral Research},
  title        = {Influence of twenty potentially antiviral substances on in vitro multiplication of hepatitis A virus},
  url          = {http://dx.doi.org/10.1016/0166-3542(86)90030-6},
  volume       = {6},
  year         = {1986},
}