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The influence of plasma insulin concentrations on tissue insulin levels in rodents: a study of the diabetic Chinese hamster and the ob/ob mouse

Agardh, Carl-David LU ; Lesniak, M A; Gerritsen, G C and Roth, J (1986) In Metabolism, Clinical and Experimental 35(3). p.244-249
Abstract
Immunoreactive insulin was measured in acid-ethanol extracts of kidney, brain, liver, and heart from genetically diabetic Chinese hamsters and their nondiabetic controls and from obese (ob/ob) mice and their thin littermates. Selected samples were filtered on Sephadex G-50 columns and the insulin concentration determined. There was a good correlation between the insulin level measured in the acid-ethanol extracts of tissues and the insulin level after gel filtration, suggesting that the concentration measured in the whole extract is representative of the true insulin content. The present data demonstrate that different extrapancreatic organs contain characteristic amounts of insulin that are often (sometimes several-fold) higher than the... (More)
Immunoreactive insulin was measured in acid-ethanol extracts of kidney, brain, liver, and heart from genetically diabetic Chinese hamsters and their nondiabetic controls and from obese (ob/ob) mice and their thin littermates. Selected samples were filtered on Sephadex G-50 columns and the insulin concentration determined. There was a good correlation between the insulin level measured in the acid-ethanol extracts of tissues and the insulin level after gel filtration, suggesting that the concentration measured in the whole extract is representative of the true insulin content. The present data demonstrate that different extrapancreatic organs contain characteristic amounts of insulin that are often (sometimes several-fold) higher than the insulin level of plasma. The tissue insulin concentrations also exhibit a wide range of values, with occasional high values. The data also show a direct correlation between plasma and kidney insulins but no relationship between plasma and brain insulins and a mixed correlation among plasma and liver and heart insulins. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Metabolism, Clinical and Experimental
volume
35
issue
3
pages
244 - 249
publisher
Elsevier
external identifiers
  • pmid:3512956
  • scopus:0022656343
ISSN
1532-8600
DOI
10.1016/0026-0495(86)90208-8
language
English
LU publication?
yes
id
fa1c4ad8-24d7-40d4-a847-c903c6da6e52 (old id 1103593)
date added to LUP
2008-08-11 09:38:38
date last changed
2017-07-30 04:42:06
@article{fa1c4ad8-24d7-40d4-a847-c903c6da6e52,
  abstract     = {Immunoreactive insulin was measured in acid-ethanol extracts of kidney, brain, liver, and heart from genetically diabetic Chinese hamsters and their nondiabetic controls and from obese (ob/ob) mice and their thin littermates. Selected samples were filtered on Sephadex G-50 columns and the insulin concentration determined. There was a good correlation between the insulin level measured in the acid-ethanol extracts of tissues and the insulin level after gel filtration, suggesting that the concentration measured in the whole extract is representative of the true insulin content. The present data demonstrate that different extrapancreatic organs contain characteristic amounts of insulin that are often (sometimes several-fold) higher than the insulin level of plasma. The tissue insulin concentrations also exhibit a wide range of values, with occasional high values. The data also show a direct correlation between plasma and kidney insulins but no relationship between plasma and brain insulins and a mixed correlation among plasma and liver and heart insulins.},
  author       = {Agardh, Carl-David and Lesniak, M A and Gerritsen, G C and Roth, J},
  issn         = {1532-8600},
  language     = {eng},
  number       = {3},
  pages        = {244--249},
  publisher    = {Elsevier},
  series       = {Metabolism, Clinical and Experimental},
  title        = {The influence of plasma insulin concentrations on tissue insulin levels in rodents: a study of the diabetic Chinese hamster and the ob/ob mouse},
  url          = {http://dx.doi.org/10.1016/0026-0495(86)90208-8},
  volume       = {35},
  year         = {1986},
}