The influence of plasma insulin concentrations on tissue insulin levels in rodents: a study of the diabetic Chinese hamster and the ob/ob mouse
(1986) In Metabolism, Clinical and Experimental 35(3). p.244-249- Abstract
- Immunoreactive insulin was measured in acid-ethanol extracts of kidney, brain, liver, and heart from genetically diabetic Chinese hamsters and their nondiabetic controls and from obese (ob/ob) mice and their thin littermates. Selected samples were filtered on Sephadex G-50 columns and the insulin concentration determined. There was a good correlation between the insulin level measured in the acid-ethanol extracts of tissues and the insulin level after gel filtration, suggesting that the concentration measured in the whole extract is representative of the true insulin content. The present data demonstrate that different extrapancreatic organs contain characteristic amounts of insulin that are often (sometimes several-fold) higher than the... (More)
- Immunoreactive insulin was measured in acid-ethanol extracts of kidney, brain, liver, and heart from genetically diabetic Chinese hamsters and their nondiabetic controls and from obese (ob/ob) mice and their thin littermates. Selected samples were filtered on Sephadex G-50 columns and the insulin concentration determined. There was a good correlation between the insulin level measured in the acid-ethanol extracts of tissues and the insulin level after gel filtration, suggesting that the concentration measured in the whole extract is representative of the true insulin content. The present data demonstrate that different extrapancreatic organs contain characteristic amounts of insulin that are often (sometimes several-fold) higher than the insulin level of plasma. The tissue insulin concentrations also exhibit a wide range of values, with occasional high values. The data also show a direct correlation between plasma and kidney insulins but no relationship between plasma and brain insulins and a mixed correlation among plasma and liver and heart insulins. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1103593
- author
- Agardh, Carl-David LU ; Lesniak, M A ; Gerritsen, G C and Roth, J
- organization
- publishing date
- 1986
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Metabolism, Clinical and Experimental
- volume
- 35
- issue
- 3
- pages
- 244 - 249
- publisher
- Elsevier
- external identifiers
-
- pmid:3512956
- scopus:0022656343
- ISSN
- 1532-8600
- DOI
- 10.1016/0026-0495(86)90208-8
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Unit on Vascular Diabetic Complications (013241510)
- id
- fa1c4ad8-24d7-40d4-a847-c903c6da6e52 (old id 1103593)
- date added to LUP
- 2016-04-01 16:51:17
- date last changed
- 2021-01-03 03:49:39
@article{fa1c4ad8-24d7-40d4-a847-c903c6da6e52,
abstract = {{Immunoreactive insulin was measured in acid-ethanol extracts of kidney, brain, liver, and heart from genetically diabetic Chinese hamsters and their nondiabetic controls and from obese (ob/ob) mice and their thin littermates. Selected samples were filtered on Sephadex G-50 columns and the insulin concentration determined. There was a good correlation between the insulin level measured in the acid-ethanol extracts of tissues and the insulin level after gel filtration, suggesting that the concentration measured in the whole extract is representative of the true insulin content. The present data demonstrate that different extrapancreatic organs contain characteristic amounts of insulin that are often (sometimes several-fold) higher than the insulin level of plasma. The tissue insulin concentrations also exhibit a wide range of values, with occasional high values. The data also show a direct correlation between plasma and kidney insulins but no relationship between plasma and brain insulins and a mixed correlation among plasma and liver and heart insulins.}},
author = {{Agardh, Carl-David and Lesniak, M A and Gerritsen, G C and Roth, J}},
issn = {{1532-8600}},
language = {{eng}},
number = {{3}},
pages = {{244--249}},
publisher = {{Elsevier}},
series = {{Metabolism, Clinical and Experimental}},
title = {{The influence of plasma insulin concentrations on tissue insulin levels in rodents: a study of the diabetic Chinese hamster and the ob/ob mouse}},
url = {{http://dx.doi.org/10.1016/0026-0495(86)90208-8}},
doi = {{10.1016/0026-0495(86)90208-8}},
volume = {{35}},
year = {{1986}},
}