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Enhancement of hepatitis A propagation in tissue culture with 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole

Widell, Anders LU ; Hansson, Bengt-Göran LU ; Nordenfelt, Erik and Öberg, Bo (1988) In Journal of Medical Virology 24(4). p.369-376
Abstract
The adenosine analog 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB) was found to increase the production of hepatitis A (HAV) antigen in two monkey kidney cell lines (Frhk-4 and Vero cells). DRB, a known inhibitor of the synthesis of messenger RNA, caused moderate changes in cell morphology. However, Frhk-4 cells could be maintained for several weeks at 80 microM of DRB, the concentration that caused maximal enhancement on HAV. DRB should be present from about the time of virus inoculation and its strongest effect was seen at low multiplicities of infection. Using radioimmunofocus assay it could be shown that DRB increased the amount of infectious virus. DRB treatment was applied in primary isolation of HAV from feces. In nine of... (More)
The adenosine analog 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB) was found to increase the production of hepatitis A (HAV) antigen in two monkey kidney cell lines (Frhk-4 and Vero cells). DRB, a known inhibitor of the synthesis of messenger RNA, caused moderate changes in cell morphology. However, Frhk-4 cells could be maintained for several weeks at 80 microM of DRB, the concentration that caused maximal enhancement on HAV. DRB should be present from about the time of virus inoculation and its strongest effect was seen at low multiplicities of infection. Using radioimmunofocus assay it could be shown that DRB increased the amount of infectious virus. DRB treatment was applied in primary isolation of HAV from feces. In nine of ten strains HAV antigen expression was strongly increased and in six of the ten strains infectivity of harvested material increased by one 10log or more. DRB thus seems to be a useful enhancer of HAV growth in tissue culture. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
virus enhancement, hepatitis A virus, HAV in tissue culture
in
Journal of Medical Virology
volume
24
issue
4
pages
369 - 376
publisher
John Wiley & Sons
external identifiers
  • pmid:2835431
  • scopus:0023941497
ISSN
1096-9071
DOI
10.1002/jmv.1890240403
language
English
LU publication?
yes
id
21944b41-7237-4272-ae8f-2f3a021b0151 (old id 1104058)
date added to LUP
2008-08-07 10:00:17
date last changed
2017-01-01 04:27:34
@article{21944b41-7237-4272-ae8f-2f3a021b0151,
  abstract     = {The adenosine analog 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB) was found to increase the production of hepatitis A (HAV) antigen in two monkey kidney cell lines (Frhk-4 and Vero cells). DRB, a known inhibitor of the synthesis of messenger RNA, caused moderate changes in cell morphology. However, Frhk-4 cells could be maintained for several weeks at 80 microM of DRB, the concentration that caused maximal enhancement on HAV. DRB should be present from about the time of virus inoculation and its strongest effect was seen at low multiplicities of infection. Using radioimmunofocus assay it could be shown that DRB increased the amount of infectious virus. DRB treatment was applied in primary isolation of HAV from feces. In nine of ten strains HAV antigen expression was strongly increased and in six of the ten strains infectivity of harvested material increased by one 10log or more. DRB thus seems to be a useful enhancer of HAV growth in tissue culture.},
  author       = {Widell, Anders and Hansson, Bengt-Göran and Nordenfelt, Erik and Öberg, Bo},
  issn         = {1096-9071},
  keyword      = {virus enhancement,hepatitis A virus,HAV in tissue culture},
  language     = {eng},
  number       = {4},
  pages        = {369--376},
  publisher    = {John Wiley & Sons},
  series       = {Journal of Medical Virology},
  title        = {Enhancement of hepatitis A propagation in tissue culture with 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole},
  url          = {http://dx.doi.org/10.1002/jmv.1890240403},
  volume       = {24},
  year         = {1988},
}