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Statistical evaluation of cell kinetic data from DNA flow cytometry (FCM) by the EM algorithm

Baldetorp, Bo LU ; Dalberg, Mats; Holst, Ulla LU and Lindgren, Georg LU (1989) In Cytometry 10(6). p.695-705
Abstract
Flow cytometric DNA measurements yield the amount of DNA for each of a large number of cells. A DNA histogram normally consists of a mixture of one or more constellations of G0/G1-, S-, G2/M-phase cells, together with internal standards, debris, background noise, and one or more populations of clumped cells. We have modelled typical DNA histograms as a mixed distribution with Gaussian densities for the G0/G1 and G2/M phases, an S-phase density, assumed to be uniform between the G0/G1 and G2/M peaks, observed with a Gaussian error, and with Gaussian densities for standards of chicken and trout red blood cells. The debris is modelled as a truncated exponential distribution, and we also have included a uniform background noise distribution... (More)
Flow cytometric DNA measurements yield the amount of DNA for each of a large number of cells. A DNA histogram normally consists of a mixture of one or more constellations of G0/G1-, S-, G2/M-phase cells, together with internal standards, debris, background noise, and one or more populations of clumped cells. We have modelled typical DNA histograms as a mixed distribution with Gaussian densities for the G0/G1 and G2/M phases, an S-phase density, assumed to be uniform between the G0/G1 and G2/M peaks, observed with a Gaussian error, and with Gaussian densities for standards of chicken and trout red blood cells. The debris is modelled as a truncated exponential distribution, and we also have included a uniform background noise distribution over the whole observation interval. We have explored a new approach for maximum-likelihood analyses of complex DNA histograms by the application of the EM algorithm. This algorithm was used for four observed DNA histograms of varying complexity. Our results show that the algorithm works very well, and it converges to reasonable values for all parameters. In simulations from the estimated models, we have investigated bias, variance, and correlations of the estimates. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
DNA-histogram analysis, maximum-likelihood estimation, cell-cycle compartments
in
Cytometry
volume
10
issue
6
pages
695 - 705
publisher
John Wiley & Sons
external identifiers
  • pmid:2582959
  • scopus:0024408530
ISSN
0196-4763
DOI
10.1002/cyto.990100605
language
English
LU publication?
yes
id
c9283803-7f84-407f-a590-6b907793ecd3 (old id 1104501)
date added to LUP
2008-08-06 09:20:18
date last changed
2017-07-30 04:26:31
@article{c9283803-7f84-407f-a590-6b907793ecd3,
  abstract     = {Flow cytometric DNA measurements yield the amount of DNA for each of a large number of cells. A DNA histogram normally consists of a mixture of one or more constellations of G0/G1-, S-, G2/M-phase cells, together with internal standards, debris, background noise, and one or more populations of clumped cells. We have modelled typical DNA histograms as a mixed distribution with Gaussian densities for the G0/G1 and G2/M phases, an S-phase density, assumed to be uniform between the G0/G1 and G2/M peaks, observed with a Gaussian error, and with Gaussian densities for standards of chicken and trout red blood cells. The debris is modelled as a truncated exponential distribution, and we also have included a uniform background noise distribution over the whole observation interval. We have explored a new approach for maximum-likelihood analyses of complex DNA histograms by the application of the EM algorithm. This algorithm was used for four observed DNA histograms of varying complexity. Our results show that the algorithm works very well, and it converges to reasonable values for all parameters. In simulations from the estimated models, we have investigated bias, variance, and correlations of the estimates.},
  author       = {Baldetorp, Bo and Dalberg, Mats and Holst, Ulla and Lindgren, Georg},
  issn         = {0196-4763},
  keyword      = {DNA-histogram analysis,maximum-likelihood estimation,cell-cycle compartments},
  language     = {eng},
  number       = {6},
  pages        = {695--705},
  publisher    = {John Wiley & Sons},
  series       = {Cytometry},
  title        = {Statistical evaluation of cell kinetic data from DNA flow cytometry (FCM) by the EM algorithm},
  url          = {http://dx.doi.org/10.1002/cyto.990100605},
  volume       = {10},
  year         = {1989},
}