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Ethanol effects on bradykinin-stimulated phosphoinositide hydrolysis in NG 108-15 neuroblastoma-glioma cells

Simonsson, Per LU ; Sun, Grace Y; Vecsei, Laszlo and Alling, Christer LU (1989) In Alcohol 6(6). p.475-479
Abstract
The effect of short-and long-term ethanol exposure on bradykinin-stimulated hydrolysis of phosphatidylinositol 4.5-bisphosphate (PIP2) was investigated in neuroblastoma X glioma hybrid cells (NG 108-15). Acute exposure of 50-150 mM ethanol neither influenced the bradykinin-stimulated accumulation of [3H]-inositol phosphates (IP1, IP2, IP3) nor the hydrolysis of PIP2 in cells labelled with [3H]-inositol. Furthermore, ethanol (100 mM) added in the absence of agonist did not influence these parameters. However, in cells cultivated for 4 days in 100 mM ethanol, PIP2 hydrolysis and IP1, IP2 and IP3 formation after stimulation by 10(-6)-10(-5) M bradykinin was markedly inhibited while there was no effect on the basal levels or on the levels... (More)
The effect of short-and long-term ethanol exposure on bradykinin-stimulated hydrolysis of phosphatidylinositol 4.5-bisphosphate (PIP2) was investigated in neuroblastoma X glioma hybrid cells (NG 108-15). Acute exposure of 50-150 mM ethanol neither influenced the bradykinin-stimulated accumulation of [3H]-inositol phosphates (IP1, IP2, IP3) nor the hydrolysis of PIP2 in cells labelled with [3H]-inositol. Furthermore, ethanol (100 mM) added in the absence of agonist did not influence these parameters. However, in cells cultivated for 4 days in 100 mM ethanol, PIP2 hydrolysis and IP1, IP2 and IP3 formation after stimulation by 10(-6)-10(-5) M bradykinin was markedly inhibited while there was no effect on the basal levels or on the levels found after stimulation with low concentrations of bradykinin. The inhibitory effect of ethanol on IP accumulation became significant after 2-3 days of ethanol. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Phosphatidylinositol 4, 5-bisphosphate, Inositol phosphates, Phospholipase C, NG 108-15 cell-line, Bradykinin, Ethanol
in
Alcohol
volume
6
issue
6
pages
475 - 479
publisher
Elsevier
external identifiers
  • pmid:2557051
  • scopus:0024820935
ISSN
0741-8329
DOI
10.1016/0741-8329(89)90055-4
language
English
LU publication?
yes
id
3fbd1c37-79ff-4d8a-b2db-95fc3d7ec668 (old id 1104749)
date added to LUP
2008-08-06 14:30:00
date last changed
2017-01-01 04:39:22
@article{3fbd1c37-79ff-4d8a-b2db-95fc3d7ec668,
  abstract     = {The effect of short-and long-term ethanol exposure on bradykinin-stimulated hydrolysis of phosphatidylinositol 4.5-bisphosphate (PIP2) was investigated in neuroblastoma X glioma hybrid cells (NG 108-15). Acute exposure of 50-150 mM ethanol neither influenced the bradykinin-stimulated accumulation of [3H]-inositol phosphates (IP1, IP2, IP3) nor the hydrolysis of PIP2 in cells labelled with [3H]-inositol. Furthermore, ethanol (100 mM) added in the absence of agonist did not influence these parameters. However, in cells cultivated for 4 days in 100 mM ethanol, PIP2 hydrolysis and IP1, IP2 and IP3 formation after stimulation by 10(-6)-10(-5) M bradykinin was markedly inhibited while there was no effect on the basal levels or on the levels found after stimulation with low concentrations of bradykinin. The inhibitory effect of ethanol on IP accumulation became significant after 2-3 days of ethanol.},
  author       = {Simonsson, Per and Sun, Grace Y and Vecsei, Laszlo and Alling, Christer},
  issn         = {0741-8329},
  keyword      = {Phosphatidylinositol 4,5-bisphosphate,Inositol phosphates,Phospholipase C,NG 108-15 cell-line,Bradykinin,Ethanol},
  language     = {eng},
  number       = {6},
  pages        = {475--479},
  publisher    = {Elsevier},
  series       = {Alcohol},
  title        = {Ethanol effects on bradykinin-stimulated phosphoinositide hydrolysis in NG 108-15 neuroblastoma-glioma cells},
  url          = {http://dx.doi.org/10.1016/0741-8329(89)90055-4},
  volume       = {6},
  year         = {1989},
}