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Effects of intravenous calcitonin gene-related peptide (CGRP) and substance P on the blood-aqueous barrier in the rabbit

Andersson, Sven LU and Bill, A (1989) In Acta Physiologica Scandinavica 135(3). p.349-357
Abstract
The irritation response of the rabbit eye to trigeminal nerve stimulation, which includes a breakdown of the blood-aqueous barrier (BAB), seems to be due to the release of substance P (SP) and calcitonin gene-related peptide (CGRP). In order to assess the relative importance of these two peptides for the barrier effect, and the role of arachidonic acid metabolites (AAM) in the response, we have studied the effects of intravenous injections of the peptides on the permeability of the blood vessels of the anterior uvea and the BAB using labelled albumins. At a dose of 120 pmol kg-1 there was marked leakage of labelled albumin into the aqueous humour in animals under pentobarbital anaesthesia. The leakage was enhanced by sympathotomy. In... (More)
The irritation response of the rabbit eye to trigeminal nerve stimulation, which includes a breakdown of the blood-aqueous barrier (BAB), seems to be due to the release of substance P (SP) and calcitonin gene-related peptide (CGRP). In order to assess the relative importance of these two peptides for the barrier effect, and the role of arachidonic acid metabolites (AAM) in the response, we have studied the effects of intravenous injections of the peptides on the permeability of the blood vessels of the anterior uvea and the BAB using labelled albumins. At a dose of 120 pmol kg-1 there was marked leakage of labelled albumin into the aqueous humour in animals under pentobarbital anaesthesia. The leakage was enhanced by sympathotomy. In conscious animals 5 pmol kg-1 CGRP caused enhanced leakage from the blood vessels of the ciliary processes in those pre-treated with biperiden in order to abolish the cholinergic vasoconstrictor tone in the anterior uvea. 24 and 120 pmol kg-1 CGRP caused marked leakage of albumin and a breakdown of the epithelial part of the BAB. These effects were not modified by biperiden pre-treatment, but markedly reduced by pre-treatment with indomethacin. The protecting effect of indomethacin was lost when biperiden was given as well. SP did not cause a leakage with 5 nmol kg-1 and only moderate leakage with 25 nmol kg-1. This effect was abolished by pre-treatment with indomethacin but not if indomethacin was combined with biperiden.(ABSTRACT TRUNCATED AT 250 WORDS) (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
in
Acta Physiologica Scandinavica
volume
135
issue
3
pages
349 - 357
publisher
Wiley-Blackwell
external identifiers
  • pmid:2467520
  • scopus:0024580348
ISSN
0001-6772
language
English
LU publication?
yes
id
4367e020-b23f-4644-a152-8c5a5c1ff20c (old id 1104925)
date added to LUP
2008-08-07 08:49:40
date last changed
2017-02-26 04:17:49
@article{4367e020-b23f-4644-a152-8c5a5c1ff20c,
  abstract     = {The irritation response of the rabbit eye to trigeminal nerve stimulation, which includes a breakdown of the blood-aqueous barrier (BAB), seems to be due to the release of substance P (SP) and calcitonin gene-related peptide (CGRP). In order to assess the relative importance of these two peptides for the barrier effect, and the role of arachidonic acid metabolites (AAM) in the response, we have studied the effects of intravenous injections of the peptides on the permeability of the blood vessels of the anterior uvea and the BAB using labelled albumins. At a dose of 120 pmol kg-1 there was marked leakage of labelled albumin into the aqueous humour in animals under pentobarbital anaesthesia. The leakage was enhanced by sympathotomy. In conscious animals 5 pmol kg-1 CGRP caused enhanced leakage from the blood vessels of the ciliary processes in those pre-treated with biperiden in order to abolish the cholinergic vasoconstrictor tone in the anterior uvea. 24 and 120 pmol kg-1 CGRP caused marked leakage of albumin and a breakdown of the epithelial part of the BAB. These effects were not modified by biperiden pre-treatment, but markedly reduced by pre-treatment with indomethacin. The protecting effect of indomethacin was lost when biperiden was given as well. SP did not cause a leakage with 5 nmol kg-1 and only moderate leakage with 25 nmol kg-1. This effect was abolished by pre-treatment with indomethacin but not if indomethacin was combined with biperiden.(ABSTRACT TRUNCATED AT 250 WORDS)},
  author       = {Andersson, Sven and Bill, A},
  issn         = {0001-6772},
  language     = {eng},
  number       = {3},
  pages        = {349--357},
  publisher    = {Wiley-Blackwell},
  series       = {Acta Physiologica Scandinavica},
  title        = {Effects of intravenous calcitonin gene-related peptide (CGRP) and substance P on the blood-aqueous barrier in the rabbit},
  volume       = {135},
  year         = {1989},
}