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Improved preparation technique of cervical carcinoma for flow cytometric DNA analysis with tissue disintegration in hydrochloric acid

Fernö, Mårten LU ; Långström-Einarsson, Eva LU ; Willen, R; Baldetorp, Bo LU and Killander, Dick LU (1990) In In Vivo 4(4). p.225-229
Abstract
An one-step procedure using a nuclear isolation medium containing propidium iodide has been found to be a suitable preparation technique for flow cytometric DNA analysis in breast cancer samples. In the case of cervical squamous carcinoma, a pretreatment with HCl seems to be a methodological improvement. One advantage with the HCl modification is that some "false" near-diploid cell populations are abolished. These "false" G0/G1 peaks may represent diploid nuclei with a different stainability for propidium iodide compared to normal diploid nuclei. The HCl treatment has, furthermore, the advantage of increasing the elution of nuclei (mean factor of 4.0), especially non-diploid nuclei from higher differentiated squamous carcinomas.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
In Vivo
volume
4
issue
4
pages
225 - 229
publisher
In vivo
external identifiers
  • pmid:2103364
  • scopus:0025200599
ISSN
0258-851X
language
English
LU publication?
yes
id
68523bba-6fe1-4bbf-8cab-e6e2d8e6688d (old id 1105006)
date added to LUP
2008-08-04 15:31:35
date last changed
2017-01-01 06:43:01
@article{68523bba-6fe1-4bbf-8cab-e6e2d8e6688d,
  abstract     = {An one-step procedure using a nuclear isolation medium containing propidium iodide has been found to be a suitable preparation technique for flow cytometric DNA analysis in breast cancer samples. In the case of cervical squamous carcinoma, a pretreatment with HCl seems to be a methodological improvement. One advantage with the HCl modification is that some "false" near-diploid cell populations are abolished. These "false" G0/G1 peaks may represent diploid nuclei with a different stainability for propidium iodide compared to normal diploid nuclei. The HCl treatment has, furthermore, the advantage of increasing the elution of nuclei (mean factor of 4.0), especially non-diploid nuclei from higher differentiated squamous carcinomas.},
  author       = {Fernö, Mårten and Långström-Einarsson, Eva and Willen, R and Baldetorp, Bo and Killander, Dick},
  issn         = {0258-851X},
  language     = {eng},
  number       = {4},
  pages        = {225--229},
  publisher    = {In vivo},
  series       = {In Vivo},
  title        = {Improved preparation technique of cervical carcinoma for flow cytometric DNA analysis with tissue disintegration in hydrochloric acid},
  volume       = {4},
  year         = {1990},
}