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Extracorporeal immunoadsorption therapy on rats. In vivo depletion of specific antibodies

Nilsson, Rune LU ; Lindgren, L and Lilliehorn, P (1990) In Clinical and Experimental Immunology 82(3). p.440-444
Abstract
A technique for extracorporeal immunoadsorption of circulating specific antibodies from the plasma of rats is described. Catheterization of rats was performed using the carotid and the jugular blood vessels. The rats were treated non-anaesthetized. Blood was pumped continuously through a hollow-fibre plasma filter at a rate of 1.5 ml/min and plasma was separated and passed through an adsorbent column at a flow of 0.2 ml/min. The treated plasma was mixed with the blood cells before being returned to the rat. The column contained the antigen covalently linked to agarose beads. About three plasma volumes were treated during a period of 3 h and 30 min, which resulted in an antibody depletion of about 90-95%. The antibody levels returned to... (More)
A technique for extracorporeal immunoadsorption of circulating specific antibodies from the plasma of rats is described. Catheterization of rats was performed using the carotid and the jugular blood vessels. The rats were treated non-anaesthetized. Blood was pumped continuously through a hollow-fibre plasma filter at a rate of 1.5 ml/min and plasma was separated and passed through an adsorbent column at a flow of 0.2 ml/min. The treated plasma was mixed with the blood cells before being returned to the rat. The column contained the antigen covalently linked to agarose beads. About three plasma volumes were treated during a period of 3 h and 30 min, which resulted in an antibody depletion of about 90-95%. The antibody levels returned to pre-adsorption levels after 4-5 days, or in some cases even exceeded the titres before treatment. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
exlracorporeal, immunoadsorplion, antibodies, rats, rebound, overshoot
in
Clinical and Experimental Immunology
volume
82
issue
3
pages
440 - 444
publisher
British Society for Immunology
external identifiers
  • pmid:2265485
  • scopus:0025241158
ISSN
0009-9104
DOI
10.1111/j.1365-2249.1990.tb05468.x
language
English
LU publication?
yes
id
671237db-6578-4fdb-88c2-b1d05a5bc378 (old id 1105125)
alternative location
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1535513
date added to LUP
2008-08-05 09:34:18
date last changed
2017-01-01 04:20:41
@article{671237db-6578-4fdb-88c2-b1d05a5bc378,
  abstract     = {A technique for extracorporeal immunoadsorption of circulating specific antibodies from the plasma of rats is described. Catheterization of rats was performed using the carotid and the jugular blood vessels. The rats were treated non-anaesthetized. Blood was pumped continuously through a hollow-fibre plasma filter at a rate of 1.5 ml/min and plasma was separated and passed through an adsorbent column at a flow of 0.2 ml/min. The treated plasma was mixed with the blood cells before being returned to the rat. The column contained the antigen covalently linked to agarose beads. About three plasma volumes were treated during a period of 3 h and 30 min, which resulted in an antibody depletion of about 90-95%. The antibody levels returned to pre-adsorption levels after 4-5 days, or in some cases even exceeded the titres before treatment.},
  author       = {Nilsson, Rune and Lindgren, L and Lilliehorn, P},
  issn         = {0009-9104},
  keyword      = {exlracorporeal,immunoadsorplion,antibodies,rats,rebound,overshoot},
  language     = {eng},
  number       = {3},
  pages        = {440--444},
  publisher    = {British Society for Immunology},
  series       = {Clinical and Experimental Immunology},
  title        = {Extracorporeal immunoadsorption therapy on rats. In vivo depletion of specific antibodies},
  url          = {http://dx.doi.org/10.1111/j.1365-2249.1990.tb05468.x},
  volume       = {82},
  year         = {1990},
}