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Polymerase chain reaction for detection of Mycobacterium tuberculosis

Sjöbring, Ulf LU ; Mecklenburg, Michael; Bengård Andersen, Åse and Miörner, Håkan LU (1990) In Journal of Clinical Microbiology 28(10). p.2200-2204
Abstract
A polymerase chain reaction for the specific detection of mycobacteria belonging to the Mycobacterium tuberculosis complex was developed. Using a single primer pair derived from the nucleotide sequence of protein antigen b of M. tuberculosis, we achieved specific amplification of a 419-base-pair DNA fragment in M. tuberculosis and M. bovis. After DNA was extracted from mycobacteria by using a simple, safe lysis procedure, we detected the 419-base-pair sequence in samples containing few mycobacteria. Preliminary data suggested that this technique could be applied to clinical specimens for early and specific diagnosis of tuberculosis.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Clinical Microbiology
volume
28
issue
10
pages
2200 - 2204
publisher
American Society for Microbiology
external identifiers
  • pmid:2121782
  • scopus:0025002931
ISSN
1098-660X
language
English
LU publication?
yes
id
c7fa6385-7680-4655-ab44-9dd2d3400f18 (old id 1105184)
alternative location
http://jcm.asm.org/cgi/reprint/28/10/2200
http://www.pubmedcentral.nih.gov/picrender.fcgi?artid=268147&blobtype=pdf
date added to LUP
2008-08-05 10:27:13
date last changed
2017-08-06 04:33:13
@article{c7fa6385-7680-4655-ab44-9dd2d3400f18,
  abstract     = {A polymerase chain reaction for the specific detection of mycobacteria belonging to the Mycobacterium tuberculosis complex was developed. Using a single primer pair derived from the nucleotide sequence of protein antigen b of M. tuberculosis, we achieved specific amplification of a 419-base-pair DNA fragment in M. tuberculosis and M. bovis. After DNA was extracted from mycobacteria by using a simple, safe lysis procedure, we detected the 419-base-pair sequence in samples containing few mycobacteria. Preliminary data suggested that this technique could be applied to clinical specimens for early and specific diagnosis of tuberculosis.},
  author       = {Sjöbring, Ulf and Mecklenburg, Michael and Bengård Andersen, Åse and Miörner, Håkan},
  issn         = {1098-660X},
  language     = {eng},
  number       = {10},
  pages        = {2200--2204},
  publisher    = {American Society for Microbiology},
  series       = {Journal of Clinical Microbiology},
  title        = {Polymerase chain reaction for detection of Mycobacterium tuberculosis},
  volume       = {28},
  year         = {1990},
}