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Polymerase chain reaction for detection of Mycobacterium tuberculosis

Sjöbring, Ulf LU ; Mecklenburg, Michael ; Bengård Andersen, Åse and Miörner, Håkan LU (1990) In Journal of Clinical Microbiology 28(10). p.2200-2204
Abstract
A polymerase chain reaction for the specific detection of mycobacteria belonging to the Mycobacterium tuberculosis complex was developed. Using a single primer pair derived from the nucleotide sequence of protein antigen b of M. tuberculosis, we achieved specific amplification of a 419-base-pair DNA fragment in M. tuberculosis and M. bovis. After DNA was extracted from mycobacteria by using a simple, safe lysis procedure, we detected the 419-base-pair sequence in samples containing few mycobacteria. Preliminary data suggested that this technique could be applied to clinical specimens for early and specific diagnosis of tuberculosis.
Please use this url to cite or link to this publication:
author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Clinical Microbiology
volume
28
issue
10
pages
2200 - 2204
publisher
American Society for Microbiology
external identifiers
  • pmid:2121782
  • scopus:0025002931
ISSN
1098-660X
language
English
LU publication?
yes
id
c7fa6385-7680-4655-ab44-9dd2d3400f18 (old id 1105184)
alternative location
http://jcm.asm.org/cgi/reprint/28/10/2200
http://www.pubmedcentral.nih.gov/picrender.fcgi?artid=268147&blobtype=pdf
date added to LUP
2016-04-01 16:21:43
date last changed
2021-09-26 03:28:58
@article{c7fa6385-7680-4655-ab44-9dd2d3400f18,
  abstract     = {{A polymerase chain reaction for the specific detection of mycobacteria belonging to the Mycobacterium tuberculosis complex was developed. Using a single primer pair derived from the nucleotide sequence of protein antigen b of M. tuberculosis, we achieved specific amplification of a 419-base-pair DNA fragment in M. tuberculosis and M. bovis. After DNA was extracted from mycobacteria by using a simple, safe lysis procedure, we detected the 419-base-pair sequence in samples containing few mycobacteria. Preliminary data suggested that this technique could be applied to clinical specimens for early and specific diagnosis of tuberculosis.}},
  author       = {{Sjöbring, Ulf and Mecklenburg, Michael and Bengård Andersen, Åse and Miörner, Håkan}},
  issn         = {{1098-660X}},
  language     = {{eng}},
  number       = {{10}},
  pages        = {{2200--2204}},
  publisher    = {{American Society for Microbiology}},
  series       = {{Journal of Clinical Microbiology}},
  title        = {{Polymerase chain reaction for detection of Mycobacterium tuberculosis}},
  url          = {{http://jcm.asm.org/cgi/reprint/28/10/2200}},
  volume       = {{28}},
  year         = {{1990}},
}