Vasoactive intestinal polypeptide in the PMSG-primed immature rat ovary and its effect on ovulation in the isolated rat ovary perfused in vitro
(1990) In Journal of Reproduction & Fertility 90(2). p.465-472- Abstract
- The immature rat ovary contains VIP immunoreactive nerve fibres sparsely distributed around blood vessels, in the interstitial gland and around follicles. The VIP concentration, measured radioimmunologically, decreased significantly after PMSG treatment (10 i.u.), probably due to ovarian enlargement and oedema, while the total VIP content (total of 0.12 pmol in both ovaries) did not change after PMSG priming. The ovulatory effect of VIP was studied using in-vitro perfused ovaries from immature 28-day-old rats primed with 10 i.u. PMSG. In all ovaries perfused, VIP (10(-7) M) induced ovulations with a rate of 2.33 +/- 0.56. The ovulation rate was significantly lower than that of ovaries stimulated by LH (0.1 microgram/ml) (5.20 +/- 0.86... (More)
- The immature rat ovary contains VIP immunoreactive nerve fibres sparsely distributed around blood vessels, in the interstitial gland and around follicles. The VIP concentration, measured radioimmunologically, decreased significantly after PMSG treatment (10 i.u.), probably due to ovarian enlargement and oedema, while the total VIP content (total of 0.12 pmol in both ovaries) did not change after PMSG priming. The ovulatory effect of VIP was studied using in-vitro perfused ovaries from immature 28-day-old rats primed with 10 i.u. PMSG. In all ovaries perfused, VIP (10(-7) M) induced ovulations with a rate of 2.33 +/- 0.56. The ovulation rate was significantly lower than that of ovaries stimulated by LH (0.1 microgram/ml) (5.20 +/- 0.86 ovulations per ovary). No synergistic effect on the ovulation rate was seen when LH and VIP were administered together (5.20 +/- 0.49 ovulations per ovary). The results suggest that the neuropeptide VIP may represent one of the local factors involved in the ovulation process. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1105314
- author
- Schmidt, G ; Jörgensen, J ; Kannisto, P LU ; Liedberg, F ; Ottesen, B and Owman, Christer LU
- organization
- publishing date
- 1990
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Reproduction & Fertility
- volume
- 90
- issue
- 2
- pages
- 465 - 472
- publisher
- Journals of Reproduction and Fertility Ltd.
- external identifiers
-
- pmid:2250246
- scopus:0025086017
- ISSN
- 0022-4251
- language
- English
- LU publication?
- yes
- id
- 63160673-2785-4c35-a62f-90f51c0400c8 (old id 1105314)
- alternative location
- https://rep.bioscientifica.com/view/journals/rep/90/2/jrf_90_2_015.xml
- date added to LUP
- 2016-04-01 16:15:51
- date last changed
- 2021-01-03 09:00:29
@article{63160673-2785-4c35-a62f-90f51c0400c8,
abstract = {{The immature rat ovary contains VIP immunoreactive nerve fibres sparsely distributed around blood vessels, in the interstitial gland and around follicles. The VIP concentration, measured radioimmunologically, decreased significantly after PMSG treatment (10 i.u.), probably due to ovarian enlargement and oedema, while the total VIP content (total of 0.12 pmol in both ovaries) did not change after PMSG priming. The ovulatory effect of VIP was studied using in-vitro perfused ovaries from immature 28-day-old rats primed with 10 i.u. PMSG. In all ovaries perfused, VIP (10(-7) M) induced ovulations with a rate of 2.33 +/- 0.56. The ovulation rate was significantly lower than that of ovaries stimulated by LH (0.1 microgram/ml) (5.20 +/- 0.86 ovulations per ovary). No synergistic effect on the ovulation rate was seen when LH and VIP were administered together (5.20 +/- 0.49 ovulations per ovary). The results suggest that the neuropeptide VIP may represent one of the local factors involved in the ovulation process.}},
author = {{Schmidt, G and Jörgensen, J and Kannisto, P and Liedberg, F and Ottesen, B and Owman, Christer}},
issn = {{0022-4251}},
language = {{eng}},
number = {{2}},
pages = {{465--472}},
publisher = {{Journals of Reproduction and Fertility Ltd.}},
series = {{Journal of Reproduction & Fertility}},
title = {{Vasoactive intestinal polypeptide in the PMSG-primed immature rat ovary and its effect on ovulation in the isolated rat ovary perfused in vitro}},
url = {{https://rep.bioscientifica.com/view/journals/rep/90/2/jrf_90_2_015.xml}},
volume = {{90}},
year = {{1990}},
}