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Vasoactive intestinal polypeptide in the PMSG-primed immature rat ovary and its effect on ovulation in the isolated rat ovary perfused in vitro

Schmidt, G; Jorgensen, J; Kannisto, P; Liedberg, F; Ottesen, B and Owman, Christer LU (1990) In Journal of Reproduction & Fertility 90(2). p.465-472
Abstract
The immature rat ovary contains VIP immunoreactive nerve fibres sparsely distributed around blood vessels, in the interstitial gland and around follicles. The VIP concentration, measured radioimmunologically, decreased significantly after PMSG treatment (10 i.u.), probably due to ovarian enlargement and oedema, while the total VIP content (total of 0.12 pmol in both ovaries) did not change after PMSG priming. The ovulatory effect of VIP was studied using in-vitro perfused ovaries from immature 28-day-old rats primed with 10 i.u. PMSG. In all ovaries perfused, VIP (10(-7) M) induced ovulations with a rate of 2.33 +/- 0.56. The ovulation rate was significantly lower than that of ovaries stimulated by LH (0.1 microgram/ml) (5.20 +/- 0.86... (More)
The immature rat ovary contains VIP immunoreactive nerve fibres sparsely distributed around blood vessels, in the interstitial gland and around follicles. The VIP concentration, measured radioimmunologically, decreased significantly after PMSG treatment (10 i.u.), probably due to ovarian enlargement and oedema, while the total VIP content (total of 0.12 pmol in both ovaries) did not change after PMSG priming. The ovulatory effect of VIP was studied using in-vitro perfused ovaries from immature 28-day-old rats primed with 10 i.u. PMSG. In all ovaries perfused, VIP (10(-7) M) induced ovulations with a rate of 2.33 +/- 0.56. The ovulation rate was significantly lower than that of ovaries stimulated by LH (0.1 microgram/ml) (5.20 +/- 0.86 ovulations per ovary). No synergistic effect on the ovulation rate was seen when LH and VIP were administered together (5.20 +/- 0.49 ovulations per ovary). The results suggest that the neuropeptide VIP may represent one of the local factors involved in the ovulation process. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Reproduction & Fertility
volume
90
issue
2
pages
465 - 472
publisher
Journals of Reproduction and Fertility Ltd.
external identifiers
  • pmid:2250246
  • scopus:0025086017
ISSN
0022-4251
language
English
LU publication?
yes
id
63160673-2785-4c35-a62f-90f51c0400c8 (old id 1105314)
date added to LUP
2008-08-05 12:28:06
date last changed
2017-08-06 04:32:07
@article{63160673-2785-4c35-a62f-90f51c0400c8,
  abstract     = {The immature rat ovary contains VIP immunoreactive nerve fibres sparsely distributed around blood vessels, in the interstitial gland and around follicles. The VIP concentration, measured radioimmunologically, decreased significantly after PMSG treatment (10 i.u.), probably due to ovarian enlargement and oedema, while the total VIP content (total of 0.12 pmol in both ovaries) did not change after PMSG priming. The ovulatory effect of VIP was studied using in-vitro perfused ovaries from immature 28-day-old rats primed with 10 i.u. PMSG. In all ovaries perfused, VIP (10(-7) M) induced ovulations with a rate of 2.33 +/- 0.56. The ovulation rate was significantly lower than that of ovaries stimulated by LH (0.1 microgram/ml) (5.20 +/- 0.86 ovulations per ovary). No synergistic effect on the ovulation rate was seen when LH and VIP were administered together (5.20 +/- 0.49 ovulations per ovary). The results suggest that the neuropeptide VIP may represent one of the local factors involved in the ovulation process.},
  author       = {Schmidt, G and Jorgensen, J and Kannisto, P and Liedberg, F and Ottesen, B and Owman, Christer},
  issn         = {0022-4251},
  language     = {eng},
  number       = {2},
  pages        = {465--472},
  publisher    = {Journals of Reproduction and Fertility Ltd.},
  series       = {Journal of Reproduction & Fertility},
  title        = {Vasoactive intestinal polypeptide in the PMSG-primed immature rat ovary and its effect on ovulation in the isolated rat ovary perfused in vitro},
  volume       = {90},
  year         = {1990},
}