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Effects of oophorectomy, sympathetic denervation and sex steroids on uterine norepinephrine content and myometrial contractile response to norepinephrine in the guinea pig

Rydhström, Håkan; Walles, Bengt and Owman, Christer LU (1990) In Neuroendocrinology 52(4). p.332-336
Abstract
Studies were performed in guinea pigs to elucidate alterations in endogenous uterine norepinephrine (NE) levels and changes in the contractile response to exogenous NE following local sympathetic denervation, oophorectomy, or treatment with sex steroids. Both in intact and oophorectomized animals the myometrial NE concentration was reduced after sex steroid treatment (0.5 microgram 17-beta-estradiol, or 0.1 microgram estradiol plus 2 mg progesterone, during 2 weeks), mainly as a result of increased uterine weight. After surgical removal of the hypogastric nerves and section of the suspensory ligaments, a similar response to sex steroids was seen if the animals had previously been oophorectomized. The myometrial contractile activity induced... (More)
Studies were performed in guinea pigs to elucidate alterations in endogenous uterine norepinephrine (NE) levels and changes in the contractile response to exogenous NE following local sympathetic denervation, oophorectomy, or treatment with sex steroids. Both in intact and oophorectomized animals the myometrial NE concentration was reduced after sex steroid treatment (0.5 microgram 17-beta-estradiol, or 0.1 microgram estradiol plus 2 mg progesterone, during 2 weeks), mainly as a result of increased uterine weight. After surgical removal of the hypogastric nerves and section of the suspensory ligaments, a similar response to sex steroids was seen if the animals had previously been oophorectomized. The myometrial contractile activity induced by exogenous NE was measured in vitro. The EC50 values (NE concentration giving 50% of the maximal response) showed a similar pattern of variations after hormonal treatment and oophorectomy as did the concentration of endogenous NE. Thus, exposure to the steroids leading to a reduction of neuronal NE also caused an increased sensitivity of the myometrial smooth musculature to exogenous NE, and in the various experimental groups the two parameters showed a close and significant relationship. The underlying mechanism may induce a denervation supersensitivity to NE induced by exposure to estrogen and progesterone. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Norepinephrine, Oophorectomy, Sympathetic denervation, Guinea pig, Myometrial contraction
in
Neuroendocrinology
volume
52
issue
4
pages
332 - 336
publisher
Karger
external identifiers
  • pmid:2124659
  • scopus:0025053249
ISSN
0028-3835
language
English
LU publication?
yes
id
48c21803-94ce-4b66-8db3-505117b54337 (old id 1105320)
date added to LUP
2008-08-05 12:30:29
date last changed
2017-01-01 04:57:37
@article{48c21803-94ce-4b66-8db3-505117b54337,
  abstract     = {Studies were performed in guinea pigs to elucidate alterations in endogenous uterine norepinephrine (NE) levels and changes in the contractile response to exogenous NE following local sympathetic denervation, oophorectomy, or treatment with sex steroids. Both in intact and oophorectomized animals the myometrial NE concentration was reduced after sex steroid treatment (0.5 microgram 17-beta-estradiol, or 0.1 microgram estradiol plus 2 mg progesterone, during 2 weeks), mainly as a result of increased uterine weight. After surgical removal of the hypogastric nerves and section of the suspensory ligaments, a similar response to sex steroids was seen if the animals had previously been oophorectomized. The myometrial contractile activity induced by exogenous NE was measured in vitro. The EC50 values (NE concentration giving 50% of the maximal response) showed a similar pattern of variations after hormonal treatment and oophorectomy as did the concentration of endogenous NE. Thus, exposure to the steroids leading to a reduction of neuronal NE also caused an increased sensitivity of the myometrial smooth musculature to exogenous NE, and in the various experimental groups the two parameters showed a close and significant relationship. The underlying mechanism may induce a denervation supersensitivity to NE induced by exposure to estrogen and progesterone.},
  author       = {Rydhström, Håkan and Walles, Bengt and Owman, Christer},
  issn         = {0028-3835},
  keyword      = {Norepinephrine,Oophorectomy,Sympathetic denervation,Guinea pig,Myometrial contraction},
  language     = {eng},
  number       = {4},
  pages        = {332--336},
  publisher    = {Karger},
  series       = {Neuroendocrinology},
  title        = {Effects of oophorectomy, sympathetic denervation and sex steroids on uterine norepinephrine content and myometrial contractile response to norepinephrine in the guinea pig},
  volume       = {52},
  year         = {1990},
}