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Stimulation of id1 expression by bone morphogenetic protein is sufficient and necessary for bone morphogenetic protein-induced activation of endothelial cells.

Valdimarsdottir, Gudrun; Goumans, Marie-José; Rosendahl, Alexander; Brugman, Martijn; Itoh, Susumu; Lebrin, Franck; Sideras, Paschalis LU and Ten Dijke, Peter (2002) In Circulation 106(17). p.2263-2270
Abstract
Background— Bone morphogenetic proteins (BMPs) are multifunctional proteins that regulate the proliferation, differentiation, and migration of a large variety of cell types. Like other members of the transforming growth factor-ß family, BMPs elicit their cellular effects through activating specific combinations of type I and type II serine/threonine kinase receptors and their downstream effector proteins, which are termed Smads. In the present study, we investigated BMP receptor/Smad expression and signaling in endothelial cells (ECs) and examined the effects of BMP on EC behavior.



Methods and Results— Immunohistochemical analysis of tissue sections of human colon and mouse heart and aorta showed that BMP receptors are... (More)
Background— Bone morphogenetic proteins (BMPs) are multifunctional proteins that regulate the proliferation, differentiation, and migration of a large variety of cell types. Like other members of the transforming growth factor-ß family, BMPs elicit their cellular effects through activating specific combinations of type I and type II serine/threonine kinase receptors and their downstream effector proteins, which are termed Smads. In the present study, we investigated BMP receptor/Smad expression and signaling in endothelial cells (ECs) and examined the effects of BMP on EC behavior.



Methods and Results— Immunohistochemical analysis of tissue sections of human colon and mouse heart and aorta showed that BMP receptors are expressed in ECs in vivo. Bovine aortic ECs and mouse embryonic ECs were found to express BMP receptors and their Smads. BMP receptor activation induced the phosphorylation of specific Smad proteins and promoted EC migration and tube formation. Id1 was identified as a BMP/Smad target in ECs. Ectopic expression of Id1 mimicked BMP-induced effects. Importantly, specific interference with Id1 expression blocked BMP-induced EC migration.



Conclusions— The BMP/Smad pathway can potently activate the endothelium. Id1 expression is strongly induced by BMP in ECs. Ectopic expression of Id1 induces EC migration and tube formation. Moreover, Id1 played a critical role in mediating BMP-induced EC migration. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
signal transduction, angiogenesis, growth substances
in
Circulation
volume
106
issue
17
pages
2263 - 2270
publisher
Lippincott Williams and Wilkins
external identifiers
  • pmid:12390958
  • wos:000178839800020
  • scopus:0037159280
ISSN
1524-4539
DOI
10.1161/01.CIR.0000033830.36431.46
language
English
LU publication?
yes
id
8a8de304-d166-4ede-9ac5-84ee54f08abf (old id 110552)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12390958&dopt=Abstract
date added to LUP
2007-06-26 13:14:34
date last changed
2017-11-05 04:22:48
@article{8a8de304-d166-4ede-9ac5-84ee54f08abf,
  abstract     = {Background— Bone morphogenetic proteins (BMPs) are multifunctional proteins that regulate the proliferation, differentiation, and migration of a large variety of cell types. Like other members of the transforming growth factor-ß family, BMPs elicit their cellular effects through activating specific combinations of type I and type II serine/threonine kinase receptors and their downstream effector proteins, which are termed Smads. In the present study, we investigated BMP receptor/Smad expression and signaling in endothelial cells (ECs) and examined the effects of BMP on EC behavior.<br/><br>
<br/><br>
Methods and Results— Immunohistochemical analysis of tissue sections of human colon and mouse heart and aorta showed that BMP receptors are expressed in ECs in vivo. Bovine aortic ECs and mouse embryonic ECs were found to express BMP receptors and their Smads. BMP receptor activation induced the phosphorylation of specific Smad proteins and promoted EC migration and tube formation. Id1 was identified as a BMP/Smad target in ECs. Ectopic expression of Id1 mimicked BMP-induced effects. Importantly, specific interference with Id1 expression blocked BMP-induced EC migration.<br/><br>
<br/><br>
Conclusions— The BMP/Smad pathway can potently activate the endothelium. Id1 expression is strongly induced by BMP in ECs. Ectopic expression of Id1 induces EC migration and tube formation. Moreover, Id1 played a critical role in mediating BMP-induced EC migration.},
  author       = {Valdimarsdottir, Gudrun and Goumans, Marie-José and Rosendahl, Alexander and Brugman, Martijn and Itoh, Susumu and Lebrin, Franck and Sideras, Paschalis and Ten Dijke, Peter},
  issn         = {1524-4539},
  keyword      = {signal transduction,angiogenesis,growth substances},
  language     = {eng},
  number       = {17},
  pages        = {2263--2270},
  publisher    = {Lippincott Williams and Wilkins},
  series       = {Circulation},
  title        = {Stimulation of id1 expression by bone morphogenetic protein is sufficient and necessary for bone morphogenetic protein-induced activation of endothelial cells.},
  url          = {http://dx.doi.org/10.1161/01.CIR.0000033830.36431.46},
  volume       = {106},
  year         = {2002},
}