Advanced

Hydrogen peroxide production in ischaemic retina: influence of hyperglycaemia and postischaemic oxygen tension

Zhang, H; Agardh, Elisabet LU and Agardh, Carl-David LU (1991) In Diabetes Research 16(1). p.29-35
Abstract
Free radicals have recently been proposed to play a role in the development of diabetic retinopathy. Ischaemia and hyperglycaemia followed by recirculation have been suggested to initiate free radical production in other tissues and the aim of the present study was to examine whether this could also be the case in the retina. The present study showed retinal cell damage, as measured by pycnotic cells, to be more pronounced when ischaemia was combined with hyperglycaemia than when combined with normoglycaemia. As an indication of free radical production, catalase activity was measured, reflecting the production of hydrogen peroxide (H2O2). Small amounts of H2O2 were found to be generated in the normal retina, but did not increase during... (More)
Free radicals have recently been proposed to play a role in the development of diabetic retinopathy. Ischaemia and hyperglycaemia followed by recirculation have been suggested to initiate free radical production in other tissues and the aim of the present study was to examine whether this could also be the case in the retina. The present study showed retinal cell damage, as measured by pycnotic cells, to be more pronounced when ischaemia was combined with hyperglycaemia than when combined with normoglycaemia. As an indication of free radical production, catalase activity was measured, reflecting the production of hydrogen peroxide (H2O2). Small amounts of H2O2 were found to be generated in the normal retina, but did not increase during ischaemia and hyperglycaemia followed by recirculation. It thus seems, as if hyperglycaemia aggravates the harmful effects of ischaemia, but with the methods used, there does not seem to be any increase in free radical production (as measured by H2O2 production) in normal rat retina during ischaemic and hyperglycaemic conditions. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Diabetes Research
volume
16
issue
1
pages
29 - 35
publisher
Teviot-Kimpton
external identifiers
  • pmid:1818795
  • scopus:0026347370
ISSN
0265-5985
language
English
LU publication?
yes
id
f6287eda-9255-4293-99db-517e412afa5e (old id 1105905)
date added to LUP
2008-08-01 14:01:22
date last changed
2017-01-01 06:53:23
@article{f6287eda-9255-4293-99db-517e412afa5e,
  abstract     = {Free radicals have recently been proposed to play a role in the development of diabetic retinopathy. Ischaemia and hyperglycaemia followed by recirculation have been suggested to initiate free radical production in other tissues and the aim of the present study was to examine whether this could also be the case in the retina. The present study showed retinal cell damage, as measured by pycnotic cells, to be more pronounced when ischaemia was combined with hyperglycaemia than when combined with normoglycaemia. As an indication of free radical production, catalase activity was measured, reflecting the production of hydrogen peroxide (H2O2). Small amounts of H2O2 were found to be generated in the normal retina, but did not increase during ischaemia and hyperglycaemia followed by recirculation. It thus seems, as if hyperglycaemia aggravates the harmful effects of ischaemia, but with the methods used, there does not seem to be any increase in free radical production (as measured by H2O2 production) in normal rat retina during ischaemic and hyperglycaemic conditions.},
  author       = {Zhang, H and Agardh, Elisabet and Agardh, Carl-David},
  issn         = {0265-5985},
  language     = {eng},
  number       = {1},
  pages        = {29--35},
  publisher    = {Teviot-Kimpton},
  series       = {Diabetes Research},
  title        = {Hydrogen peroxide production in ischaemic retina: influence of hyperglycaemia and postischaemic oxygen tension},
  volume       = {16},
  year         = {1991},
}