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Continuous and intermittent exposure to ethanol: effect on NG 108-15 cell membrane phospholipids

Alling, Christer LU ; Rodriguez, F D; Gustavsson, L and Simonsson, Per LU (1991) In Alcohol and alcoholism. Supplement 1. p.227-231
Abstract
The effect of continuous and intermittent ethanol exposure on the phospholipid composition of Neuroblastoma x Glioma (NG 108-15) cell membranes was investigated. The cells were treated with ethanol for three weeks. Continuous ethanol exposure (150 mM) produced an increase (27%) in the amount of phosphatidylcholine, whereas intermittent ethanol treatment (150 mM) induced a 22% reduction of this lipid. Decreases of phosphatidylethanolamine plasmalogen (8.5%), phosphatidylinositol (16%) and phosphatidylserine (24%) were also seen after intermittent exposure. After binge administration, the concentration of total phospholipids was reduced by 17%, whereas continuous exposure produced a 19% increase. Both intermittent and continuous exposure... (More)
The effect of continuous and intermittent ethanol exposure on the phospholipid composition of Neuroblastoma x Glioma (NG 108-15) cell membranes was investigated. The cells were treated with ethanol for three weeks. Continuous ethanol exposure (150 mM) produced an increase (27%) in the amount of phosphatidylcholine, whereas intermittent ethanol treatment (150 mM) induced a 22% reduction of this lipid. Decreases of phosphatidylethanolamine plasmalogen (8.5%), phosphatidylinositol (16%) and phosphatidylserine (24%) were also seen after intermittent exposure. After binge administration, the concentration of total phospholipids was reduced by 17%, whereas continuous exposure produced a 19% increase. Both intermittent and continuous exposure induced a reduction in the total protein content. No changes in phosphatidic acid, sphingomyelin, phosphatidylcholine plasmalogen or phosphatidylethanolamine (diacyl form) were detected with either treatment. The importance of this study is that ethanol, irrespective of amount, can elicit different effects depending on the pattern of administration. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
in
Alcohol and alcoholism. Supplement
volume
1
pages
227 - 231
publisher
Pergamon
external identifiers
  • pmid:1845541
  • scopus:0026379874
ISSN
1358-6173
language
English
LU publication?
yes
id
ded6c66b-7fd7-4a6c-9569-800aa9ea3c2b (old id 1106005)
date added to LUP
2008-08-01 13:51:44
date last changed
2017-01-01 06:53:08
@article{ded6c66b-7fd7-4a6c-9569-800aa9ea3c2b,
  abstract     = {The effect of continuous and intermittent ethanol exposure on the phospholipid composition of Neuroblastoma x Glioma (NG 108-15) cell membranes was investigated. The cells were treated with ethanol for three weeks. Continuous ethanol exposure (150 mM) produced an increase (27%) in the amount of phosphatidylcholine, whereas intermittent ethanol treatment (150 mM) induced a 22% reduction of this lipid. Decreases of phosphatidylethanolamine plasmalogen (8.5%), phosphatidylinositol (16%) and phosphatidylserine (24%) were also seen after intermittent exposure. After binge administration, the concentration of total phospholipids was reduced by 17%, whereas continuous exposure produced a 19% increase. Both intermittent and continuous exposure induced a reduction in the total protein content. No changes in phosphatidic acid, sphingomyelin, phosphatidylcholine plasmalogen or phosphatidylethanolamine (diacyl form) were detected with either treatment. The importance of this study is that ethanol, irrespective of amount, can elicit different effects depending on the pattern of administration.},
  author       = {Alling, Christer and Rodriguez, F D and Gustavsson, L and Simonsson, Per},
  issn         = {1358-6173},
  language     = {eng},
  pages        = {227--231},
  publisher    = {Pergamon},
  series       = {Alcohol and alcoholism. Supplement},
  title        = {Continuous and intermittent exposure to ethanol: effect on NG 108-15 cell membrane phospholipids},
  volume       = {1},
  year         = {1991},
}