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Hepatitis C virus antibodies and hepatitis C virus RNA in Chinese blood donors determined by ELISA, recombinant immunoblot assay and polymerase chain reaction

Zhang, Yong-Yuan; Hansson, B G; Widell, Anders LU and Nordenfelt, E (1992) In Acta Pathologica, Microbiologica et Immunologica Scandinavica (APMIS) 100(9). p.851-855
Abstract
Antibodies to hepatitis C virus (anti-HCV) were determined in Chinese blood donors from the city of Wuhan by a second generation ELISA screening test and a confirmatory recombinant immunoblot assay (RIBA II). Two materials of 281 and 222 sera were sampled under similar conditions in 1989 and 1990, respectively. The first collection of sera was sent to Sweden in lyophilized form, the second directly as fresh unfrozen sera. A high proportion (7.1%) of the lyophilized sera reacted positively in the anti-HCV screening assay, but only seven (2.5%) were positive by the RIBA confirmatory test. In four of these sera HCV-RNA could be detected by polymerase chain reaction (PCR) analysis. In the second material of fresh sera six reacted positively in... (More)
Antibodies to hepatitis C virus (anti-HCV) were determined in Chinese blood donors from the city of Wuhan by a second generation ELISA screening test and a confirmatory recombinant immunoblot assay (RIBA II). Two materials of 281 and 222 sera were sampled under similar conditions in 1989 and 1990, respectively. The first collection of sera was sent to Sweden in lyophilized form, the second directly as fresh unfrozen sera. A high proportion (7.1%) of the lyophilized sera reacted positively in the anti-HCV screening assay, but only seven (2.5%) were positive by the RIBA confirmatory test. In four of these sera HCV-RNA could be detected by polymerase chain reaction (PCR) analysis. In the second material of fresh sera six reacted positively in the screening anti-HCV ELISA, but only one was RIBA positive and four were RIBA indeterminate. None of these sera was positive for HCV-RNA. Thus, the overall prevalence of anti-HCV among the 503 Chinese blood donors, as identified by RIBA, was 1.6%, and of HCV-RNA by PCR 0.8%. The confirmed antibody prevalence is higher than reported from the Western world. Caution about using data from the screening ELISA only, especially if sera have been handled in an unorthodox way, is emphasized. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Acta Pathologica, Microbiologica et Immunologica Scandinavica (APMIS)
volume
100
issue
9
pages
851 - 855
publisher
John Wiley & Sons
external identifiers
  • pmid:1382461
  • scopus:0026730881
ISSN
1600-0463
language
English
LU publication?
yes
id
1d408987-0677-4e4f-b73d-732044e82489 (old id 1106184)
date added to LUP
2008-08-11 15:41:45
date last changed
2017-01-01 05:19:32
@article{1d408987-0677-4e4f-b73d-732044e82489,
  abstract     = {Antibodies to hepatitis C virus (anti-HCV) were determined in Chinese blood donors from the city of Wuhan by a second generation ELISA screening test and a confirmatory recombinant immunoblot assay (RIBA II). Two materials of 281 and 222 sera were sampled under similar conditions in 1989 and 1990, respectively. The first collection of sera was sent to Sweden in lyophilized form, the second directly as fresh unfrozen sera. A high proportion (7.1%) of the lyophilized sera reacted positively in the anti-HCV screening assay, but only seven (2.5%) were positive by the RIBA confirmatory test. In four of these sera HCV-RNA could be detected by polymerase chain reaction (PCR) analysis. In the second material of fresh sera six reacted positively in the screening anti-HCV ELISA, but only one was RIBA positive and four were RIBA indeterminate. None of these sera was positive for HCV-RNA. Thus, the overall prevalence of anti-HCV among the 503 Chinese blood donors, as identified by RIBA, was 1.6%, and of HCV-RNA by PCR 0.8%. The confirmed antibody prevalence is higher than reported from the Western world. Caution about using data from the screening ELISA only, especially if sera have been handled in an unorthodox way, is emphasized.},
  author       = {Zhang, Yong-Yuan and Hansson, B G and Widell, Anders and Nordenfelt, E},
  issn         = {1600-0463},
  language     = {eng},
  number       = {9},
  pages        = {851--855},
  publisher    = {John Wiley & Sons},
  series       = {Acta Pathologica, Microbiologica et Immunologica Scandinavica (APMIS)},
  title        = {Hepatitis C virus antibodies and hepatitis C virus RNA in Chinese blood donors determined by ELISA, recombinant immunoblot assay and polymerase chain reaction},
  volume       = {100},
  year         = {1992},
}