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Detection of nephropathia epidemica virus RNA in patient samples using a nested primer-based polymerase chain reaction

Grankvist, O; Juto, P; Settergren, B; Ahlm, C; Bjermer, Leif LU ; Linderholm, M; Tarnvik, A and Wadell, G (1992) In Journal of Infectious Diseases 165(5). p.934-937
Abstract
A nested primer-based polymerase chain reaction was constructed for the detection of Puumala virus RNA in patient samples. Puumala virus RNA was detected in cells from the urinary and the respiratory tracts and in peripheral blood mononuclear cells of patients with nephropathia epidemica. After inoculation with nephropathia epidemica patient material on Vero E6 cells and propagation for nine passages (4 months), Puumala virus RNA was detected at every passage. Hybridization under high-stringency conditions revealed that the overall nucleotide homology between the different patient isolates and the prototype strain (Puumala) is high. Using cDNA from Hallnas B1 strain as a probe, hybridization occurred only under low-stringency conditions.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Infectious Diseases
volume
165
issue
5
pages
934 - 937
publisher
Oxford University Press
external identifiers
  • pmid:1349033
  • scopus:0026511254
ISSN
1537-6613
language
English
LU publication?
yes
id
ddbc921a-6063-4c8e-be22-2632f93646f0 (old id 1106352)
date added to LUP
2008-08-01 15:36:54
date last changed
2017-08-27 05:32:56
@article{ddbc921a-6063-4c8e-be22-2632f93646f0,
  abstract     = {A nested primer-based polymerase chain reaction was constructed for the detection of Puumala virus RNA in patient samples. Puumala virus RNA was detected in cells from the urinary and the respiratory tracts and in peripheral blood mononuclear cells of patients with nephropathia epidemica. After inoculation with nephropathia epidemica patient material on Vero E6 cells and propagation for nine passages (4 months), Puumala virus RNA was detected at every passage. Hybridization under high-stringency conditions revealed that the overall nucleotide homology between the different patient isolates and the prototype strain (Puumala) is high. Using cDNA from Hallnas B1 strain as a probe, hybridization occurred only under low-stringency conditions.},
  author       = {Grankvist, O and Juto, P and Settergren, B and Ahlm, C and Bjermer, Leif and Linderholm, M and Tarnvik, A and Wadell, G},
  issn         = {1537-6613},
  language     = {eng},
  number       = {5},
  pages        = {934--937},
  publisher    = {Oxford University Press},
  series       = {Journal of Infectious Diseases},
  title        = {Detection of nephropathia epidemica virus RNA in patient samples using a nested primer-based polymerase chain reaction},
  volume       = {165},
  year         = {1992},
}