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Purification and properties of the cGMP-inhibited cAMP phosphodiesterase from bovine aortic smooth muscle

Rascon, Ana LU ; Lindgren, Sam; Stavenow, Lars; Belfrage, Per LU ; Andersson, Karl-Erik; Manganiello, Vincent C and Degerman, Eva (1992) In Biochimica et Biophysica Acta 1134(2). p.149-156
Abstract
Pure cGMP-inhibited cAMP phosphodiesterase (cGI-PDE) in micrograms quantities was isolated from bovine aortic smooth muscle after more than 5000-fold purification using DEAE ion-exchange and affinity chromatography with a derivative of the specific cGI-PDE inhibitor cilostamide conjugated as a ligand to aminoethyl agarose (CIT-agarose). The cGI-PDE, which constituted about half of the high affinity cAMP-PDE activity of a tissue homogenate, was identified with a 105-kDa protein on SDS-PAGE through use of antibodies towards the human platelet, bovine cardiac and bovine adipose tissue cGI-PDE in Western blot and immunoprecipitation/immunoinactivation analysis. As observed during purification of the enzyme from other tissues the enzyme protein... (More)
Pure cGMP-inhibited cAMP phosphodiesterase (cGI-PDE) in micrograms quantities was isolated from bovine aortic smooth muscle after more than 5000-fold purification using DEAE ion-exchange and affinity chromatography with a derivative of the specific cGI-PDE inhibitor cilostamide conjugated as a ligand to aminoethyl agarose (CIT-agarose). The cGI-PDE, which constituted about half of the high affinity cAMP-PDE activity of a tissue homogenate, was identified with a 105-kDa protein on SDS-PAGE through use of antibodies towards the human platelet, bovine cardiac and bovine adipose tissue cGI-PDE in Western blot and immunoprecipitation/immunoinactivation analysis. As observed during purification of the enzyme from other tissues the enzyme protein was exquisitely sensitive to proteolytic nicking during purification, resulting in several 30-77-kDa polypeptide fragments. Rapid immunoprecipitation from fresh tissue extracts was the only was found to partially prevent the proteolysis. The native enzyme had apparent molecular sizes of approx. 100,000 or, mainly approx. 220,000 by gel chromatography, presumably indicating the presence of monomeric and dimeric forms. The enzyme hydrolyzed cAMP and cGMP with normal Michaelis-Menten kinetics with Km of 0.16 and 0.09 microM, respectively, with Vmax for hydrolysis of cAMP of 0.3 compared to 3.1 mumol/min per mg protein for cAMP. The enzyme was potently and selectively inhibited by cGMP (IC50 approximately 0.25 microM) and the cardiotonic/vasodilatory drugs OPC-3911 (a cilostamide derivative), milrinone and CI-930 (IC50 approximately 0.05, 0.40 and 0.25 microM, respectively). The cGI-PDE was phosphorylated by cAMP-dependent protein kinase as has been reported for the analogous enzymes in heart, adipose tissue and platelets. The identification of a cGI-PDE in the aortic smooth muscle and its inhibitor specificity is consistent with the hypothesis that inhibition of this enzyme is important in the mechanism through which these drugs produce vasorelaxation. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Smooth muscle, cGMP-inhibited cyclic nucleotide phosphodiesterase, Phosphodiesterase inhibitor, (Bovine aorta)
in
Biochimica et Biophysica Acta
volume
1134
issue
2
pages
149 - 156
publisher
Elsevier
external identifiers
  • pmid:1313303
  • scopus:0026531771
ISSN
0006-3002
DOI
10.1016/0167-4889(92)90038-D
language
English
LU publication?
yes
id
c898069a-1865-4657-95ed-427b65591aa9 (old id 1106745)
date added to LUP
2008-08-12 09:10:53
date last changed
2017-08-27 05:24:21
@article{c898069a-1865-4657-95ed-427b65591aa9,
  abstract     = {Pure cGMP-inhibited cAMP phosphodiesterase (cGI-PDE) in micrograms quantities was isolated from bovine aortic smooth muscle after more than 5000-fold purification using DEAE ion-exchange and affinity chromatography with a derivative of the specific cGI-PDE inhibitor cilostamide conjugated as a ligand to aminoethyl agarose (CIT-agarose). The cGI-PDE, which constituted about half of the high affinity cAMP-PDE activity of a tissue homogenate, was identified with a 105-kDa protein on SDS-PAGE through use of antibodies towards the human platelet, bovine cardiac and bovine adipose tissue cGI-PDE in Western blot and immunoprecipitation/immunoinactivation analysis. As observed during purification of the enzyme from other tissues the enzyme protein was exquisitely sensitive to proteolytic nicking during purification, resulting in several 30-77-kDa polypeptide fragments. Rapid immunoprecipitation from fresh tissue extracts was the only was found to partially prevent the proteolysis. The native enzyme had apparent molecular sizes of approx. 100,000 or, mainly approx. 220,000 by gel chromatography, presumably indicating the presence of monomeric and dimeric forms. The enzyme hydrolyzed cAMP and cGMP with normal Michaelis-Menten kinetics with Km of 0.16 and 0.09 microM, respectively, with Vmax for hydrolysis of cAMP of 0.3 compared to 3.1 mumol/min per mg protein for cAMP. The enzyme was potently and selectively inhibited by cGMP (IC50 approximately 0.25 microM) and the cardiotonic/vasodilatory drugs OPC-3911 (a cilostamide derivative), milrinone and CI-930 (IC50 approximately 0.05, 0.40 and 0.25 microM, respectively). The cGI-PDE was phosphorylated by cAMP-dependent protein kinase as has been reported for the analogous enzymes in heart, adipose tissue and platelets. The identification of a cGI-PDE in the aortic smooth muscle and its inhibitor specificity is consistent with the hypothesis that inhibition of this enzyme is important in the mechanism through which these drugs produce vasorelaxation.},
  author       = {Rascon, Ana and Lindgren, Sam and Stavenow, Lars and Belfrage, Per and Andersson, Karl-Erik and Manganiello, Vincent C and Degerman, Eva},
  issn         = {0006-3002},
  keyword      = {Smooth muscle,cGMP-inhibited cyclic nucleotide phosphodiesterase,Phosphodiesterase inhibitor,(Bovine aorta)},
  language     = {eng},
  number       = {2},
  pages        = {149--156},
  publisher    = {Elsevier},
  series       = {Biochimica et Biophysica Acta},
  title        = {Purification and properties of the cGMP-inhibited cAMP phosphodiesterase from bovine aortic smooth muscle},
  url          = {http://dx.doi.org/10.1016/0167-4889(92)90038-D},
  volume       = {1134},
  year         = {1992},
}