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A method for maintaining constant ethanol concentrations in cell culture media

Rodriguez, F David; Simonsson, Per LU and Alling, Christer LU (1992) In Alcohol and Alcoholism 27(3). p.309-313
Abstract
The present study reports on the development of a model for maintaining constant ethanol concentrations over time in cell culture media. When neuroblastoma x glioma cells (NG 108-15) were grown in ethanol containing media under standard cultivation conditions in the incubator at 37 degrees C, a 90% evaporation was observed after 24 hr. To counteract evaporation, the cell culture dishes were placed inside polystyrene boxes together with an open dish containing an appropriate amount of ethanol. By using such procedure, the decrease in ethanol concentration in the culture media was completely avoided. Cultivating cells in ethanol-free media inside sealed plastic boxes did not change their viability, growth rate, protein and phospholipid... (More)
The present study reports on the development of a model for maintaining constant ethanol concentrations over time in cell culture media. When neuroblastoma x glioma cells (NG 108-15) were grown in ethanol containing media under standard cultivation conditions in the incubator at 37 degrees C, a 90% evaporation was observed after 24 hr. To counteract evaporation, the cell culture dishes were placed inside polystyrene boxes together with an open dish containing an appropriate amount of ethanol. By using such procedure, the decrease in ethanol concentration in the culture media was completely avoided. Cultivating cells in ethanol-free media inside sealed plastic boxes did not change their viability, growth rate, protein and phospholipid composition of the cells or the pH of the media, compared to cultures grown outside the boxes. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Alcohol and Alcoholism
volume
27
issue
3
pages
309 - 313
publisher
Oxford University Press
external identifiers
  • pmid:1449566
  • scopus:0026646519
ISSN
1464-3502
language
English
LU publication?
yes
id
addda95d-613f-41af-9fb9-d0a2be2bd637 (old id 1106773)
alternative location
http://alcalc.oxfordjournals.org/cgi/reprint/27/3/309
date added to LUP
2008-08-12 09:27:03
date last changed
2017-01-01 04:41:54
@article{addda95d-613f-41af-9fb9-d0a2be2bd637,
  abstract     = {The present study reports on the development of a model for maintaining constant ethanol concentrations over time in cell culture media. When neuroblastoma x glioma cells (NG 108-15) were grown in ethanol containing media under standard cultivation conditions in the incubator at 37 degrees C, a 90% evaporation was observed after 24 hr. To counteract evaporation, the cell culture dishes were placed inside polystyrene boxes together with an open dish containing an appropriate amount of ethanol. By using such procedure, the decrease in ethanol concentration in the culture media was completely avoided. Cultivating cells in ethanol-free media inside sealed plastic boxes did not change their viability, growth rate, protein and phospholipid composition of the cells or the pH of the media, compared to cultures grown outside the boxes.},
  author       = {Rodriguez, F David and Simonsson, Per and Alling, Christer},
  issn         = {1464-3502},
  language     = {eng},
  number       = {3},
  pages        = {309--313},
  publisher    = {Oxford University Press},
  series       = {Alcohol and Alcoholism},
  title        = {A method for maintaining constant ethanol concentrations in cell culture media},
  volume       = {27},
  year         = {1992},
}