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Preprogastrin-releasing peptide messenger ribonucleic acid: neuroanatomical localization in rat brain by in situ hybridization with synthetic oligodeoxynucleotide probes

Gundlach, A L and Knobe, Karin LU (1992) In Neuroscience Letters 137(1). p.123-128
Abstract
Gastrin-releasing peptide (GRP) is a 27 amino acid peptide that is present in both the central and peripheral nervous systems and that shares immunological and functional properties with the amphibian peptide, bombesin. GRP has multiple putative biological functions including effects on feeding behaviour and carbohydrate metabolism, body temperature, and effects on hormone release, but little is known about the regulation of GRP gene expression in the brain. This study examined the distribution of neurones expressing preproGRP mRNA in rat brain by in situ hybridization of [35S]-labelled DNA oligonucleotides. PreproGRP mRNA was detected in several regions of brain, with highest concentrations in the parvocellular paraventricular and... (More)
Gastrin-releasing peptide (GRP) is a 27 amino acid peptide that is present in both the central and peripheral nervous systems and that shares immunological and functional properties with the amphibian peptide, bombesin. GRP has multiple putative biological functions including effects on feeding behaviour and carbohydrate metabolism, body temperature, and effects on hormone release, but little is known about the regulation of GRP gene expression in the brain. This study examined the distribution of neurones expressing preproGRP mRNA in rat brain by in situ hybridization of [35S]-labelled DNA oligonucleotides. PreproGRP mRNA was detected in several regions of brain, with highest concentrations in the parvocellular paraventricular and suprachiasmatic nuclei of the hypothalamus, the lateral and basolateral nuclei of the amygdala, the amygdaloid-hippocampal area and the ventral part of the granule cell layer of the dentate gyrus. Moderate levels were seen in layers II and III of the cingulate and retrosplenial cortex, the medial and mediobasal nuclei of the amygdala, the anteroventral thalamic nucleus; medial geniculate nucleus and the parabrachial nucleus. These findings are largely consistent with the cellular localization of GRP-like immunoreactivity in rat brain and recent studies of preproGRP mRNA localization using cRNA probes. The distribution of preproGRP mRNA observed further suggests the involvement of GRP in the central regulation of several functions including regulation of hypothalamic/pituitary hormone release. (Less)
Please use this url to cite or link to this publication:
author
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Brain (vertebrata), Gastrin releasing peptide, Messenger RNA, Gene expression, Bombesin, Molecular hybridization, In situ, Rat, Central nervous system, Hormone releasing factor, Peptide hormone, Rodentia, Mammalia, Vertebrata
in
Neuroscience Letters
volume
137
issue
1
pages
123 - 128
publisher
Elsevier
external identifiers
  • pmid:1625811
  • scopus:0026578894
ISSN
0304-3940
language
English
LU publication?
no
id
b8743b0a-f095-4e9b-88b2-f9c4b9e5fe09 (old id 1106823)
date added to LUP
2008-08-01 15:45:18
date last changed
2017-01-01 05:10:30
@article{b8743b0a-f095-4e9b-88b2-f9c4b9e5fe09,
  abstract     = {Gastrin-releasing peptide (GRP) is a 27 amino acid peptide that is present in both the central and peripheral nervous systems and that shares immunological and functional properties with the amphibian peptide, bombesin. GRP has multiple putative biological functions including effects on feeding behaviour and carbohydrate metabolism, body temperature, and effects on hormone release, but little is known about the regulation of GRP gene expression in the brain. This study examined the distribution of neurones expressing preproGRP mRNA in rat brain by in situ hybridization of [35S]-labelled DNA oligonucleotides. PreproGRP mRNA was detected in several regions of brain, with highest concentrations in the parvocellular paraventricular and suprachiasmatic nuclei of the hypothalamus, the lateral and basolateral nuclei of the amygdala, the amygdaloid-hippocampal area and the ventral part of the granule cell layer of the dentate gyrus. Moderate levels were seen in layers II and III of the cingulate and retrosplenial cortex, the medial and mediobasal nuclei of the amygdala, the anteroventral thalamic nucleus; medial geniculate nucleus and the parabrachial nucleus. These findings are largely consistent with the cellular localization of GRP-like immunoreactivity in rat brain and recent studies of preproGRP mRNA localization using cRNA probes. The distribution of preproGRP mRNA observed further suggests the involvement of GRP in the central regulation of several functions including regulation of hypothalamic/pituitary hormone release.},
  author       = {Gundlach, A L and Knobe, Karin},
  issn         = {0304-3940},
  keyword      = {Brain (vertebrata),Gastrin releasing peptide,Messenger RNA,Gene expression,Bombesin,Molecular hybridization,In situ,Rat,Central nervous system,Hormone releasing factor,Peptide hormone,Rodentia,Mammalia,Vertebrata},
  language     = {eng},
  number       = {1},
  pages        = {123--128},
  publisher    = {Elsevier},
  series       = {Neuroscience Letters},
  title        = {Preprogastrin-releasing peptide messenger ribonucleic acid: neuroanatomical localization in rat brain by in situ hybridization with synthetic oligodeoxynucleotide probes},
  volume       = {137},
  year         = {1992},
}