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Effects of low-dose X-irradiation on mouse-brain aggregation cultures

Dimberg, Y; Tottmar, O; Aspberg, Anders LU ; Ebendal, T; Johansson, K J and Walinder, G (1992) In International Journal of Radiation Biology 61(3). p.355-363
Abstract
Biochemical and morphological differentiation in reaggregating mouse-brain cell cultures after low-dose radiation (0.5 Gy) in vitro was studied. Cells were irradiated on culture day 2, corresponding to embryonic day 15-16, and different glial and neuronal markers were followed through development to postnatal day 40. The shape and size of irradiated aggregates were more irregular and smaller compared with controls. Total amounts of DNA and protein were significantly lower in irradiated aggregates than in controls between days 8 and 20. After 30 days in culture activities of the glial markers glutamine synthetase (GS) and 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNP) were lower in X-irradiated aggregates than in controls. However,... (More)
Biochemical and morphological differentiation in reaggregating mouse-brain cell cultures after low-dose radiation (0.5 Gy) in vitro was studied. Cells were irradiated on culture day 2, corresponding to embryonic day 15-16, and different glial and neuronal markers were followed through development to postnatal day 40. The shape and size of irradiated aggregates were more irregular and smaller compared with controls. Total amounts of DNA and protein were significantly lower in irradiated aggregates than in controls between days 8 and 20. After 30 days in culture activities of the glial markers glutamine synthetase (GS) and 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNP) were lower in X-irradiated aggregates than in controls. However, after 40 days the CNP activity in irradiated aggregates increased to levels above those of the controls. Irradiated and control aggregates did not differ significantly in neuronal marker enzyme activities, i.e. choline acetyltransferase (ChAT), acetylcholine esterase (AChE) and glutamic acid decarboxylase (GAD) measured on a per mg protein basis. On days 20 and 30 the amount of nerve growth factor (NGF) was two-fold higher in irradiated aggregates compared with non-irradiated ones, suggesting that, after irradiation, surviving cells in culture were induced to produce more NGF. After 40 days the amount of NGF in irradiated aggregates had decreased to the level found in the control aggregates. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
International Journal of Radiation Biology
volume
61
issue
3
pages
355 - 363
publisher
Taylor & Francis
external identifiers
  • pmid:1347068
  • scopus:0026567311
ISSN
0955-3002
DOI
10.1080/09553009214551041
language
English
LU publication?
yes
id
772b4f12-17c0-4cc4-b546-bf1bed935f35 (old id 1106904)
date added to LUP
2008-07-31 11:16:48
date last changed
2017-07-30 03:32:28
@article{772b4f12-17c0-4cc4-b546-bf1bed935f35,
  abstract     = {Biochemical and morphological differentiation in reaggregating mouse-brain cell cultures after low-dose radiation (0.5 Gy) in vitro was studied. Cells were irradiated on culture day 2, corresponding to embryonic day 15-16, and different glial and neuronal markers were followed through development to postnatal day 40. The shape and size of irradiated aggregates were more irregular and smaller compared with controls. Total amounts of DNA and protein were significantly lower in irradiated aggregates than in controls between days 8 and 20. After 30 days in culture activities of the glial markers glutamine synthetase (GS) and 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNP) were lower in X-irradiated aggregates than in controls. However, after 40 days the CNP activity in irradiated aggregates increased to levels above those of the controls. Irradiated and control aggregates did not differ significantly in neuronal marker enzyme activities, i.e. choline acetyltransferase (ChAT), acetylcholine esterase (AChE) and glutamic acid decarboxylase (GAD) measured on a per mg protein basis. On days 20 and 30 the amount of nerve growth factor (NGF) was two-fold higher in irradiated aggregates compared with non-irradiated ones, suggesting that, after irradiation, surviving cells in culture were induced to produce more NGF. After 40 days the amount of NGF in irradiated aggregates had decreased to the level found in the control aggregates.},
  author       = {Dimberg, Y and Tottmar, O and Aspberg, Anders and Ebendal, T and Johansson, K J and Walinder, G},
  issn         = {0955-3002},
  language     = {eng},
  number       = {3},
  pages        = {355--363},
  publisher    = {Taylor & Francis},
  series       = {International Journal of Radiation Biology},
  title        = {Effects of low-dose X-irradiation on mouse-brain aggregation cultures},
  url          = {http://dx.doi.org/10.1080/09553009214551041},
  volume       = {61},
  year         = {1992},
}