Effects of low-dose X-irradiation on mouse-brain aggregation cultures
Dimberg, Y ; Tottmar, O ; Aspberg, Anders LU
; Ebendal, T
; Johansson, K J
and Walinder, G
(1992)
In International Journal of Radiation Biology
61(3).
p.355-363
- Abstract
- Biochemical and morphological differentiation in reaggregating mouse-brain cell cultures after low-dose radiation (0.5 Gy) in vitro was studied. Cells were irradiated on culture day 2, corresponding to embryonic day 15-16, and different glial and neuronal markers were followed through development to postnatal day 40. The shape and size of irradiated aggregates were more irregular and smaller compared with controls. Total amounts of DNA and protein were significantly lower in irradiated aggregates than in controls between days 8 and 20. After 30 days in culture activities of the glial markers glutamine synthetase (GS) and 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNP) were lower in X-irradiated aggregates than in controls. However,... (More)
- Biochemical and morphological differentiation in reaggregating mouse-brain cell cultures after low-dose radiation (0.5 Gy) in vitro was studied. Cells were irradiated on culture day 2, corresponding to embryonic day 15-16, and different glial and neuronal markers were followed through development to postnatal day 40. The shape and size of irradiated aggregates were more irregular and smaller compared with controls. Total amounts of DNA and protein were significantly lower in irradiated aggregates than in controls between days 8 and 20. After 30 days in culture activities of the glial markers glutamine synthetase (GS) and 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNP) were lower in X-irradiated aggregates than in controls. However, after 40 days the CNP activity in irradiated aggregates increased to levels above those of the controls. Irradiated and control aggregates did not differ significantly in neuronal marker enzyme activities, i.e. choline acetyltransferase (ChAT), acetylcholine esterase (AChE) and glutamic acid decarboxylase (GAD) measured on a per mg protein basis. On days 20 and 30 the amount of nerve growth factor (NGF) was two-fold higher in irradiated aggregates compared with non-irradiated ones, suggesting that, after irradiation, surviving cells in culture were induced to produce more NGF. After 40 days the amount of NGF in irradiated aggregates had decreased to the level found in the control aggregates. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1106904
- author
- Dimberg, Y
; Tottmar, O
; Aspberg, Anders
LU
; Ebendal, T
; Johansson, K J
and Walinder, G
- organization
- publishing date
- 1992
- type
- Contribution to journal
- publication status
- published
- subject
- in
- International Journal of Radiation Biology
- volume
- 61
- issue
- 3
- pages
- 355 - 363
- publisher
- Taylor & Francis
- external identifiers
-
- pmid:1347068
- scopus:0026567311
- ISSN
- 0955-3002
- DOI
- 10.1080/09553009214551041
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Connective Tissue Biology (013230151)
- id
- 772b4f12-17c0-4cc4-b546-bf1bed935f35 (old id 1106904)
- date added to LUP
- 2016-04-01 11:39:33
- date last changed
- 2021-01-03 06:27:36
@article{772b4f12-17c0-4cc4-b546-bf1bed935f35,
abstract = {{Biochemical and morphological differentiation in reaggregating mouse-brain cell cultures after low-dose radiation (0.5 Gy) in vitro was studied. Cells were irradiated on culture day 2, corresponding to embryonic day 15-16, and different glial and neuronal markers were followed through development to postnatal day 40. The shape and size of irradiated aggregates were more irregular and smaller compared with controls. Total amounts of DNA and protein were significantly lower in irradiated aggregates than in controls between days 8 and 20. After 30 days in culture activities of the glial markers glutamine synthetase (GS) and 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNP) were lower in X-irradiated aggregates than in controls. However, after 40 days the CNP activity in irradiated aggregates increased to levels above those of the controls. Irradiated and control aggregates did not differ significantly in neuronal marker enzyme activities, i.e. choline acetyltransferase (ChAT), acetylcholine esterase (AChE) and glutamic acid decarboxylase (GAD) measured on a per mg protein basis. On days 20 and 30 the amount of nerve growth factor (NGF) was two-fold higher in irradiated aggregates compared with non-irradiated ones, suggesting that, after irradiation, surviving cells in culture were induced to produce more NGF. After 40 days the amount of NGF in irradiated aggregates had decreased to the level found in the control aggregates.}},
author = {{Dimberg, Y and Tottmar, O and Aspberg, Anders and Ebendal, T and Johansson, K J and Walinder, G}},
issn = {{0955-3002}},
language = {{eng}},
number = {{3}},
pages = {{355--363}},
publisher = {{Taylor & Francis}},
series = {{International Journal of Radiation Biology}},
title = {{Effects of low-dose X-irradiation on mouse-brain aggregation cultures}},
url = {{http://dx.doi.org/10.1080/09553009214551041}},
doi = {{10.1080/09553009214551041}},
volume = {{61}},
year = {{1992}},
}