Also with a restrictive transfusion policy, screening with second-generation anti-hepatitis C virus enzyme-linked immunosorbent assay would have reduced post-transfusion hepatitis C after open-heart surgery
(1993) In Scandinavian Journal of Gastroenterology 28(7). p.581-584- Abstract
- The incidence of post-transfusion hepatitis non-A, non-B (PTH-NANB) was prospectively assessed among open-heart surgery patients from the southeast region of Sweden before the introduction of antihepatitis C virus (HCV) blood donor screening. Blood samples for alanine aminotransferase analysis were drawn before and 2, 3, and 4 months after transfusion. Surgery was performed in four centres. Of 190 transfused and followed-up patients 2 (1.1%) contracted PTH-NANB, both operated on at the centre with significantly fewer transfusions than the other centres. One patient had antibodies to HCV detected by first-generation (C100-3) and later by second-generation anti-HCV enzyme-linked immunosorbent assay (ELISA-2) and by positive second-generation... (More)
- The incidence of post-transfusion hepatitis non-A, non-B (PTH-NANB) was prospectively assessed among open-heart surgery patients from the southeast region of Sweden before the introduction of antihepatitis C virus (HCV) blood donor screening. Blood samples for alanine aminotransferase analysis were drawn before and 2, 3, and 4 months after transfusion. Surgery was performed in four centres. Of 190 transfused and followed-up patients 2 (1.1%) contracted PTH-NANB, both operated on at the centre with significantly fewer transfusions than the other centres. One patient had antibodies to HCV detected by first-generation (C100-3) and later by second-generation anti-HCV enzyme-linked immunosorbent assay (ELISA-2) and by positive second-generation recombinant immunoblot assay (4-RIBA). The other patient, although negative by first-generation anti-HCV ELISA, was positive by second-generation ELISA and by 4-RIBA. Both patients were hepatitis C-viremic by polymerase chain reaction (PCR). All the six donors implicated in the two hepatitis cases were first-generation anti-HCV-negative, but two, one for each patient, were positive by second-generation anti-HCV ELISA. This finding was confirmed by positive 4-RIBA in only 1 donor, the other being 'indeterminate'. However, in both donors hepatitis C viremia was found by PCR. This study shows that the second-generation anti-HCV ELISA will further reduce the risk for PTH-NANB/C and draws attention to the problem of evaluation of confirmatory tests. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1106961
- author
- Mathiesen, U L ; Karlsson, E ; Foberg, U ; Fryden, A ; Franzen, L ; Widell, Anders LU and Bodemar, G
- organization
- publishing date
- 1993
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- C22-3-indeterminate, polymerase chain reaction, post-transfusion hepatitis non-A, non-B, post-transfusion hepatitis C, second-generation anti-hepatitis C virus screening, second-generation recombinant immunoblot assay
- in
- Scandinavian Journal of Gastroenterology
- volume
- 28
- issue
- 7
- pages
- 581 - 584
- publisher
- Taylor & Francis
- external identifiers
-
- pmid:7689744
- scopus:0027272055
- ISSN
- 1502-7708
- DOI
- 10.3109/00365529309096091
- language
- English
- LU publication?
- yes
- id
- 4ba3df7b-a3a9-470f-9cc4-2de2bfda71ab (old id 1106961)
- date added to LUP
- 2016-04-01 15:30:52
- date last changed
- 2021-01-03 06:40:46
@article{4ba3df7b-a3a9-470f-9cc4-2de2bfda71ab, abstract = {{The incidence of post-transfusion hepatitis non-A, non-B (PTH-NANB) was prospectively assessed among open-heart surgery patients from the southeast region of Sweden before the introduction of antihepatitis C virus (HCV) blood donor screening. Blood samples for alanine aminotransferase analysis were drawn before and 2, 3, and 4 months after transfusion. Surgery was performed in four centres. Of 190 transfused and followed-up patients 2 (1.1%) contracted PTH-NANB, both operated on at the centre with significantly fewer transfusions than the other centres. One patient had antibodies to HCV detected by first-generation (C100-3) and later by second-generation anti-HCV enzyme-linked immunosorbent assay (ELISA-2) and by positive second-generation recombinant immunoblot assay (4-RIBA). The other patient, although negative by first-generation anti-HCV ELISA, was positive by second-generation ELISA and by 4-RIBA. Both patients were hepatitis C-viremic by polymerase chain reaction (PCR). All the six donors implicated in the two hepatitis cases were first-generation anti-HCV-negative, but two, one for each patient, were positive by second-generation anti-HCV ELISA. This finding was confirmed by positive 4-RIBA in only 1 donor, the other being 'indeterminate'. However, in both donors hepatitis C viremia was found by PCR. This study shows that the second-generation anti-HCV ELISA will further reduce the risk for PTH-NANB/C and draws attention to the problem of evaluation of confirmatory tests.}}, author = {{Mathiesen, U L and Karlsson, E and Foberg, U and Fryden, A and Franzen, L and Widell, Anders and Bodemar, G}}, issn = {{1502-7708}}, keywords = {{C22-3-indeterminate; polymerase chain reaction; post-transfusion hepatitis non-A; non-B; post-transfusion hepatitis C; second-generation anti-hepatitis C virus screening; second-generation recombinant immunoblot assay}}, language = {{eng}}, number = {{7}}, pages = {{581--584}}, publisher = {{Taylor & Francis}}, series = {{Scandinavian Journal of Gastroenterology}}, title = {{Also with a restrictive transfusion policy, screening with second-generation anti-hepatitis C virus enzyme-linked immunosorbent assay would have reduced post-transfusion hepatitis C after open-heart surgery}}, url = {{http://dx.doi.org/10.3109/00365529309096091}}, doi = {{10.3109/00365529309096091}}, volume = {{28}}, year = {{1993}}, }