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Also with a restrictive transfusion policy, screening with second-generation anti-hepatitis C virus enzyme-linked immunosorbent assay would have reduced post-transfusion hepatitis C after open-heart surgery

Mathiesen, U L; Karlsson, E; Foberg, U; Fryden, A; Franzen, L; Widell, Anders LU and Bodemar, G (1993) In Scandinavian Journal of Gastroenterology 28(7). p.581-584
Abstract
The incidence of post-transfusion hepatitis non-A, non-B (PTH-NANB) was prospectively assessed among open-heart surgery patients from the southeast region of Sweden before the introduction of antihepatitis C virus (HCV) blood donor screening. Blood samples for alanine aminotransferase analysis were drawn before and 2, 3, and 4 months after transfusion. Surgery was performed in four centres. Of 190 transfused and followed-up patients 2 (1.1%) contracted PTH-NANB, both operated on at the centre with significantly fewer transfusions than the other centres. One patient had antibodies to HCV detected by first-generation (C100-3) and later by second-generation anti-HCV enzyme-linked immunosorbent assay (ELISA-2) and by positive second-generation... (More)
The incidence of post-transfusion hepatitis non-A, non-B (PTH-NANB) was prospectively assessed among open-heart surgery patients from the southeast region of Sweden before the introduction of antihepatitis C virus (HCV) blood donor screening. Blood samples for alanine aminotransferase analysis were drawn before and 2, 3, and 4 months after transfusion. Surgery was performed in four centres. Of 190 transfused and followed-up patients 2 (1.1%) contracted PTH-NANB, both operated on at the centre with significantly fewer transfusions than the other centres. One patient had antibodies to HCV detected by first-generation (C100-3) and later by second-generation anti-HCV enzyme-linked immunosorbent assay (ELISA-2) and by positive second-generation recombinant immunoblot assay (4-RIBA). The other patient, although negative by first-generation anti-HCV ELISA, was positive by second-generation ELISA and by 4-RIBA. Both patients were hepatitis C-viremic by polymerase chain reaction (PCR). All the six donors implicated in the two hepatitis cases were first-generation anti-HCV-negative, but two, one for each patient, were positive by second-generation anti-HCV ELISA. This finding was confirmed by positive 4-RIBA in only 1 donor, the other being 'indeterminate'. However, in both donors hepatitis C viremia was found by PCR. This study shows that the second-generation anti-HCV ELISA will further reduce the risk for PTH-NANB/C and draws attention to the problem of evaluation of confirmatory tests. (Less)
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Contribution to journal
publication status
published
subject
keywords
C22-3-indeterminate, polymerase chain reaction, post-transfusion hepatitis non-A, non-B, post-transfusion hepatitis C, second-generation anti-hepatitis C virus screening, second-generation recombinant immunoblot assay
in
Scandinavian Journal of Gastroenterology
volume
28
issue
7
pages
581 - 584
publisher
Taylor & Francis
external identifiers
  • pmid:7689744
  • scopus:0027272055
ISSN
1502-7708
DOI
10.3109/00365529309096091
language
English
LU publication?
yes
id
4ba3df7b-a3a9-470f-9cc4-2de2bfda71ab (old id 1106961)
date added to LUP
2008-07-30 08:45:17
date last changed
2017-01-01 06:41:11
@article{4ba3df7b-a3a9-470f-9cc4-2de2bfda71ab,
  abstract     = {The incidence of post-transfusion hepatitis non-A, non-B (PTH-NANB) was prospectively assessed among open-heart surgery patients from the southeast region of Sweden before the introduction of antihepatitis C virus (HCV) blood donor screening. Blood samples for alanine aminotransferase analysis were drawn before and 2, 3, and 4 months after transfusion. Surgery was performed in four centres. Of 190 transfused and followed-up patients 2 (1.1%) contracted PTH-NANB, both operated on at the centre with significantly fewer transfusions than the other centres. One patient had antibodies to HCV detected by first-generation (C100-3) and later by second-generation anti-HCV enzyme-linked immunosorbent assay (ELISA-2) and by positive second-generation recombinant immunoblot assay (4-RIBA). The other patient, although negative by first-generation anti-HCV ELISA, was positive by second-generation ELISA and by 4-RIBA. Both patients were hepatitis C-viremic by polymerase chain reaction (PCR). All the six donors implicated in the two hepatitis cases were first-generation anti-HCV-negative, but two, one for each patient, were positive by second-generation anti-HCV ELISA. This finding was confirmed by positive 4-RIBA in only 1 donor, the other being 'indeterminate'. However, in both donors hepatitis C viremia was found by PCR. This study shows that the second-generation anti-HCV ELISA will further reduce the risk for PTH-NANB/C and draws attention to the problem of evaluation of confirmatory tests.},
  author       = {Mathiesen, U L and Karlsson, E and Foberg, U and Fryden, A and Franzen, L and Widell, Anders and Bodemar, G},
  issn         = {1502-7708},
  keyword      = {C22-3-indeterminate,polymerase chain reaction,post-transfusion hepatitis non-A,non-B,post-transfusion hepatitis C,second-generation anti-hepatitis C virus screening,second-generation recombinant immunoblot assay},
  language     = {eng},
  number       = {7},
  pages        = {581--584},
  publisher    = {Taylor & Francis},
  series       = {Scandinavian Journal of Gastroenterology},
  title        = {Also with a restrictive transfusion policy, screening with second-generation anti-hepatitis C virus enzyme-linked immunosorbent assay would have reduced post-transfusion hepatitis C after open-heart surgery},
  url          = {http://dx.doi.org/10.3109/00365529309096091},
  volume       = {28},
  year         = {1993},
}