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Antibodies to hepatitis C virus and hepatitis C virus RNA in Chinese blood donors determined by ELISA, recombinant immunoblot assay and polymerase chain reaction

Zhang, Yong-Yuan; Guo, L S; Hao, L J; Hansson, Bengt-Göran LU ; Widell, Anders LU and Nordenfelt, Erik (1993) In Chinese Medical Journal 106(3). p.171-174
Abstract
Antibodies to hepatitis C virus (anti-HCV) were determined in Chinese blood donors from the city of Wuhan by ELISA screening tests and confirmatory recombinant immunoblot assay (RIBA). 281 and 222 sera were sampled under similar conditions in 1989 and 1990, respectively. The first collection of sera was sent to Sweden in lyophilized form, the second directly as fresh, unfrozen sera. A high proportion (22%) of the lyophilized sera were positive in the screening assay but only 6 (2.10%) were positive by RIBA with antibodies against both the C100-3 and 5-1-1 peptides. HCV RNA could be detected by polymerase chain reaction (PCR) analysis in 3 of the 6 sera and in one reacting with C100-3 only. In the second material of fresh sera only 3 were... (More)
Antibodies to hepatitis C virus (anti-HCV) were determined in Chinese blood donors from the city of Wuhan by ELISA screening tests and confirmatory recombinant immunoblot assay (RIBA). 281 and 222 sera were sampled under similar conditions in 1989 and 1990, respectively. The first collection of sera was sent to Sweden in lyophilized form, the second directly as fresh, unfrozen sera. A high proportion (22%) of the lyophilized sera were positive in the screening assay but only 6 (2.10%) were positive by RIBA with antibodies against both the C100-3 and 5-1-1 peptides. HCV RNA could be detected by polymerase chain reaction (PCR) analysis in 3 of the 6 sera and in one reacting with C100-3 only. In the second material of fresh sera only 3 were positive in the screening anti-HCV ELISA, but none was RIBA or PCR positive. Thus, the overall prevalence of anti-HCV among the 503 Chinese blood donors as identified by RIBA was 1.2%, and that of HCV RNA by PCR was 0.8%. The confirmed antibody prevalence is higher than that reported in the western literature. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Chinese Medical Journal
volume
106
issue
3
pages
171 - 174
publisher
Chinese Medical Association
external identifiers
  • pmid:7686839
  • scopus:0027569992
ISSN
0366-6999
language
English
LU publication?
yes
id
00f6bd2c-799a-45aa-9236-3b5bbc2cb97b (old id 1106973)
date added to LUP
2008-07-30 08:52:49
date last changed
2017-07-30 04:31:48
@article{00f6bd2c-799a-45aa-9236-3b5bbc2cb97b,
  abstract     = {Antibodies to hepatitis C virus (anti-HCV) were determined in Chinese blood donors from the city of Wuhan by ELISA screening tests and confirmatory recombinant immunoblot assay (RIBA). 281 and 222 sera were sampled under similar conditions in 1989 and 1990, respectively. The first collection of sera was sent to Sweden in lyophilized form, the second directly as fresh, unfrozen sera. A high proportion (22%) of the lyophilized sera were positive in the screening assay but only 6 (2.10%) were positive by RIBA with antibodies against both the C100-3 and 5-1-1 peptides. HCV RNA could be detected by polymerase chain reaction (PCR) analysis in 3 of the 6 sera and in one reacting with C100-3 only. In the second material of fresh sera only 3 were positive in the screening anti-HCV ELISA, but none was RIBA or PCR positive. Thus, the overall prevalence of anti-HCV among the 503 Chinese blood donors as identified by RIBA was 1.2%, and that of HCV RNA by PCR was 0.8%. The confirmed antibody prevalence is higher than that reported in the western literature.},
  author       = {Zhang, Yong-Yuan and Guo, L S and Hao, L J and Hansson, Bengt-Göran and Widell, Anders and Nordenfelt, Erik},
  issn         = {0366-6999},
  language     = {eng},
  number       = {3},
  pages        = {171--174},
  publisher    = {Chinese Medical Association},
  series       = {Chinese Medical Journal},
  title        = {Antibodies to hepatitis C virus and hepatitis C virus RNA in Chinese blood donors determined by ELISA, recombinant immunoblot assay and polymerase chain reaction},
  volume       = {106},
  year         = {1993},
}