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Context dependence of protein secondary structure formation: the three-dimensional structure and stability of a hybrid between chymotrypsin inhibitor 2 and helix E from subtilisin Carlsberg

Osmark, Peter LU ; Sorensen, Poul and Poulsen, Flemming M (1993) In Biochemistry 32(41). p.11007-11014
Abstract
The loop region of chymotrypsin inhibitor 2 from barley has been employed as a scaffold for testing the intrinsic propensity of a peptide fragment to form a secondary structure. The helix formation of the nine amino acid residue segment Lys-Gln- Ala-Val- Asp- Asn- Ala-Tyr-Ala of helix E from subtilisin Carlsberg has been studied by the construction of a hybrid consisting of chymotrypsin inhibitor 2 (CI2) where part of the active loop has been replaced by the nonapeptide. An expression system for a truncated form of C12 where the 19 structureless residues of the N-terminus have been removed and Leu20 replaced

by methionyl was constructed from the entire 83-residue wild-type C12 gene by polymerase chain reaction

... (More)
The loop region of chymotrypsin inhibitor 2 from barley has been employed as a scaffold for testing the intrinsic propensity of a peptide fragment to form a secondary structure. The helix formation of the nine amino acid residue segment Lys-Gln- Ala-Val- Asp- Asn- Ala-Tyr-Ala of helix E from subtilisin Carlsberg has been studied by the construction of a hybrid consisting of chymotrypsin inhibitor 2 (CI2) where part of the active loop has been replaced by the nonapeptide. An expression system for a truncated form of C12 where the 19 structureless residues of the N-terminus have been removed and Leu20 replaced

by methionyl was constructed from the entire 83-residue wild-type C12 gene by polymerase chain reaction

methodology. The gene encoding the hybrid was constructed from the truncated inhibitor gene. The stability of the truncated inhibitor and of the hybrid toward guanidinium chloride denaturation was examined. From these measurements, the energy of unfolding in pure water was extrapolated to 30.5 1.0 kJ/mol for the truncated inhibitor and 10.9 f 0.3 kJ/mol for the hybrid. These energies show that the stability of C12 is unaffected by the N-terminal truncation but severely decreased by the loop mutations. The three-dimensional structure of the hybrid protein has been determined in solution by nuclear magnetic

resonance spectroscopy using 893 distance restraints and 84 torsional angle restraints. The average rootmean-square deviation (rmsd) of 15 structures compared to their geometrical average was 0.8 f 0.2 A for heavy backbone atoms and 1.3 * 0.2 A for all heavy atoms. The inserted peptide segment does not form an a-helix in the new structural context whereas the structure of the C12 scaffold turns out to be amazingly conserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biochemistry
volume
32
issue
41
pages
11007 - 11014
publisher
The American Chemical Society
external identifiers
  • pmid:8218165
  • scopus:0027427995
ISSN
0006-2960
DOI
10.1021/bi00092a009
language
English
LU publication?
yes
id
1e7da19b-137f-4fcf-9f74-7f6d6b4d2ece (old id 1107005)
date added to LUP
2008-07-30 09:15:44
date last changed
2017-07-23 03:47:25
@article{1e7da19b-137f-4fcf-9f74-7f6d6b4d2ece,
  abstract     = {The loop region of chymotrypsin inhibitor 2 from barley has been employed as a scaffold for testing the intrinsic propensity of a peptide fragment to form a secondary structure. The helix formation of the nine amino acid residue segment Lys-Gln- Ala-Val- Asp- Asn- Ala-Tyr-Ala of helix E from subtilisin Carlsberg has been studied by the construction of a hybrid consisting of chymotrypsin inhibitor 2 (CI2) where part of the active loop has been replaced by the nonapeptide. An expression system for a truncated form of C12 where the 19 structureless residues of the N-terminus have been removed and Leu20 replaced<br/><br>
by methionyl was constructed from the entire 83-residue wild-type C12 gene by polymerase chain reaction<br/><br>
methodology. The gene encoding the hybrid was constructed from the truncated inhibitor gene. The stability of the truncated inhibitor and of the hybrid toward guanidinium chloride denaturation was examined. From these measurements, the energy of unfolding in pure water was extrapolated to 30.5 1.0 kJ/mol for the truncated inhibitor and 10.9 f 0.3 kJ/mol for the hybrid. These energies show that the stability of C12 is unaffected by the N-terminal truncation but severely decreased by the loop mutations. The three-dimensional structure of the hybrid protein has been determined in solution by nuclear magnetic<br/><br>
resonance spectroscopy using 893 distance restraints and 84 torsional angle restraints. The average rootmean-square deviation (rmsd) of 15 structures compared to their geometrical average was 0.8 f 0.2 A for heavy backbone atoms and 1.3 * 0.2 A for all heavy atoms. The inserted peptide segment does not form an a-helix in the new structural context whereas the structure of the C12 scaffold turns out to be amazingly conserved.},
  author       = {Osmark, Peter and Sorensen, Poul and Poulsen, Flemming M},
  issn         = {0006-2960},
  language     = {eng},
  number       = {41},
  pages        = {11007--11014},
  publisher    = {The American Chemical Society},
  series       = {Biochemistry},
  title        = {Context dependence of protein secondary structure formation: the three-dimensional structure and stability of a hybrid between chymotrypsin inhibitor 2 and helix E from subtilisin Carlsberg},
  url          = {http://dx.doi.org/10.1021/bi00092a009},
  volume       = {32},
  year         = {1993},
}