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In vitro toxicity of biomaterials determined with cell density, total protein, cell cycle distribution and adenine nucleotides

Wieslander, Anders P; Nordin, Marika K; Hansson, Björn; Baldetorp, Bo LU and Kjellstrand, Per T T (1993) In Artificial Cells, Blood Substitutes, and Biotechnology 21(1). p.63-70
Abstract
Inhibition of cell growth is the most commonly used endpoint for in vitro toxicity of biomaterials. The use of several different endpoints might however generate more information concerning the nature of the toxicity. Thus, we examined the toxicity of two biomaterials, Polyvinylchloride (PVC) and Polyoximethene (POM), with different selected endpoints. The influence of cell growth on these endpoints was also investigated. Water extracts from the polymeric materials were tested on the continuous cell line L-929. Cell density, total protein, total protein per cell, fraction of cells in G0/G1- or S-phase, the concentration of ATP, ADP and AMP were used as endpoints. The PVC material did not significantly influence any of these endpoints until... (More)
Inhibition of cell growth is the most commonly used endpoint for in vitro toxicity of biomaterials. The use of several different endpoints might however generate more information concerning the nature of the toxicity. Thus, we examined the toxicity of two biomaterials, Polyvinylchloride (PVC) and Polyoximethene (POM), with different selected endpoints. The influence of cell growth on these endpoints was also investigated. Water extracts from the polymeric materials were tested on the continuous cell line L-929. Cell density, total protein, total protein per cell, fraction of cells in G0/G1- or S-phase, the concentration of ATP, ADP and AMP were used as endpoints. The PVC material did not significantly influence any of these endpoints until after 72 hours of exposure and the main part of the toxicity at 72 hours was related to higher proliferation rate in control cultures. After the cells had been incubated for 8 hour with POM the main toxic effect was on the energy parameters. In conclusion the PVC material was less toxic than the POM material. Our results also implies that the choice of endpoint will influence the evaluation of cytotoxicity. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Artificial Cells, Blood Substitutes, and Biotechnology
volume
21
issue
1
pages
63 - 70
publisher
Informa Healthcare
external identifiers
  • pmid:8461437
  • scopus:0027406647
ISSN
1055-7172
DOI
10.3109/10731199309118297
language
English
LU publication?
yes
id
472cd146-c1b9-4950-9016-ee935d86ee0a (old id 1107064)
date added to LUP
2008-07-30 10:19:03
date last changed
2017-01-01 06:59:42
@article{472cd146-c1b9-4950-9016-ee935d86ee0a,
  abstract     = {Inhibition of cell growth is the most commonly used endpoint for in vitro toxicity of biomaterials. The use of several different endpoints might however generate more information concerning the nature of the toxicity. Thus, we examined the toxicity of two biomaterials, Polyvinylchloride (PVC) and Polyoximethene (POM), with different selected endpoints. The influence of cell growth on these endpoints was also investigated. Water extracts from the polymeric materials were tested on the continuous cell line L-929. Cell density, total protein, total protein per cell, fraction of cells in G0/G1- or S-phase, the concentration of ATP, ADP and AMP were used as endpoints. The PVC material did not significantly influence any of these endpoints until after 72 hours of exposure and the main part of the toxicity at 72 hours was related to higher proliferation rate in control cultures. After the cells had been incubated for 8 hour with POM the main toxic effect was on the energy parameters. In conclusion the PVC material was less toxic than the POM material. Our results also implies that the choice of endpoint will influence the evaluation of cytotoxicity.},
  author       = {Wieslander, Anders P and Nordin, Marika K and Hansson, Björn and Baldetorp, Bo and Kjellstrand, Per T T},
  issn         = {1055-7172},
  language     = {eng},
  number       = {1},
  pages        = {63--70},
  publisher    = {Informa Healthcare},
  series       = {Artificial Cells, Blood Substitutes, and Biotechnology},
  title        = {In vitro toxicity of biomaterials determined with cell density, total protein, cell cycle distribution and adenine nucleotides},
  url          = {http://dx.doi.org/10.3109/10731199309118297},
  volume       = {21},
  year         = {1993},
}