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Improved removal of anti-A and anti-B antibodies from plasma using blood-group-active haptens

Blomberg, L; Bratt, T; Chester, Alan LU and Hansson, B (1993) In Vox Sanguinis 65(2). p.126-135
Abstract
Highly efficient anti-blood group A and B immunoadsorbents for extracorporeal treatment were developed by immobilizing A and B trisaccharide and A tetrasaccharide haptens on Sepharose 4FF and Fractogel TSK using three different methods. The adsorption of the IgG and IgM anti-A antibodies was essentially the same regardless of the A hapten used or the method of oligosaccharide coupling. The adsorption of IgM anti-A was found to be more sensitive than IgG anti-A to changes in column flow rate. The binding of both the IgM and IgG antibodies was slightly lower at 37 degrees C than at 22 degrees C. An anti-A/anti-B adsorbent column potentially suitable for treatment of patients was prepared. A column switching system resulted in a more... (More)
Highly efficient anti-blood group A and B immunoadsorbents for extracorporeal treatment were developed by immobilizing A and B trisaccharide and A tetrasaccharide haptens on Sepharose 4FF and Fractogel TSK using three different methods. The adsorption of the IgG and IgM anti-A antibodies was essentially the same regardless of the A hapten used or the method of oligosaccharide coupling. The adsorption of IgM anti-A was found to be more sensitive than IgG anti-A to changes in column flow rate. The binding of both the IgM and IgG antibodies was slightly lower at 37 degrees C than at 22 degrees C. An anti-A/anti-B adsorbent column potentially suitable for treatment of patients was prepared. A column switching system resulted in a more efficient adsorption of antibodies. Hapten leakage from the column was very low. No nonspecific adsorption of plasma proteins to the column (other than traces of albumin) could be detected. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Vox Sanguinis
volume
65
issue
2
pages
126 - 135
publisher
Federation of European Neuroscience Societies and Blackwell Publishing Ltd
external identifiers
  • pmid:8212667
  • scopus:0027327035
ISSN
1423-0410
language
English
LU publication?
yes
id
d306b1c1-2d8b-451d-832b-eed34953007a (old id 1107152)
date added to LUP
2008-07-30 12:24:39
date last changed
2017-01-01 07:12:00
@article{d306b1c1-2d8b-451d-832b-eed34953007a,
  abstract     = {Highly efficient anti-blood group A and B immunoadsorbents for extracorporeal treatment were developed by immobilizing A and B trisaccharide and A tetrasaccharide haptens on Sepharose 4FF and Fractogel TSK using three different methods. The adsorption of the IgG and IgM anti-A antibodies was essentially the same regardless of the A hapten used or the method of oligosaccharide coupling. The adsorption of IgM anti-A was found to be more sensitive than IgG anti-A to changes in column flow rate. The binding of both the IgM and IgG antibodies was slightly lower at 37 degrees C than at 22 degrees C. An anti-A/anti-B adsorbent column potentially suitable for treatment of patients was prepared. A column switching system resulted in a more efficient adsorption of antibodies. Hapten leakage from the column was very low. No nonspecific adsorption of plasma proteins to the column (other than traces of albumin) could be detected.},
  author       = {Blomberg, L and Bratt, T and Chester, Alan and Hansson, B},
  issn         = {1423-0410},
  language     = {eng},
  number       = {2},
  pages        = {126--135},
  publisher    = {Federation of European Neuroscience Societies and Blackwell Publishing Ltd},
  series       = {Vox Sanguinis},
  title        = {Improved removal of anti-A and anti-B antibodies from plasma using blood-group-active haptens},
  volume       = {65},
  year         = {1993},
}