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Improving radioimmonotargeting of tumors. Variation in the amount of L6 MAb administered, combined with an immunoadsorption system (ECIA)

Garkavij, Michael LU ; Tennvall, Jan LU ; Strand, Sven-Erik LU ; Norrgren, Kristina LU ; Nilsson, Rune LU ; Lindgren, Lars and Sjögren, Hans Olov LU (1993) In Acta Oncologica 32(7-8). p.853-859
Abstract
Extracorporeal immunoadsorption (ECIA) is a new method for the selective removal of circulating radiolabeled monoclonal antibodies (MAb) from plasma to increase the uptake in tumor versus normal tissues (T/N-ratio). To ascertain whether the amount of MAb affects T/N ratios immediately and 24 h after ECIA, we used a rat model with two tumor sites--one intramuscular (im) and one below the subrenal capsule (SR). Extracorporeal immunoadsorption was done with an avidin-agarose column after injection of 125I-labeled biotinylated L6 MAb. The animals received 10, 50 or 250 micrograms of L6 only (controls), or followed by ECIA. The efficacy of the procedure in removing plasma activity was 80-95%. For both tumor sites, the highest T/N-ratios were... (More)
Extracorporeal immunoadsorption (ECIA) is a new method for the selective removal of circulating radiolabeled monoclonal antibodies (MAb) from plasma to increase the uptake in tumor versus normal tissues (T/N-ratio). To ascertain whether the amount of MAb affects T/N ratios immediately and 24 h after ECIA, we used a rat model with two tumor sites--one intramuscular (im) and one below the subrenal capsule (SR). Extracorporeal immunoadsorption was done with an avidin-agarose column after injection of 125I-labeled biotinylated L6 MAb. The animals received 10, 50 or 250 micrograms of L6 only (controls), or followed by ECIA. The efficacy of the procedure in removing plasma activity was 80-95%. For both tumor sites, the highest T/N-ratios were obtained with 10 micrograms L6. All T/N-ratios significantly improved for SR tumors by a factor ranging from 3.2 (lung) to 12.6 (bone marrow). The T/N-ratios were still elevated 24 h after ECIA. Injection of larger amounts of MAb, probably causing a higher degree of tumor saturation, will not necessarily improve the T/N ratio after ECIA. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Acta Oncologica
volume
32
issue
7-8
pages
853 - 859
publisher
Taylor & Francis
external identifiers
  • pmid:8305236
  • scopus:0027786702
ISSN
1651-226X
DOI
10.3109/02841869309096146
language
English
LU publication?
yes
id
fed8cbe4-6f2c-4308-8c1c-2494cd9ea8b3 (old id 1107160)
date added to LUP
2008-07-30 12:33:38
date last changed
2017-01-01 06:37:01
@article{fed8cbe4-6f2c-4308-8c1c-2494cd9ea8b3,
  abstract     = {Extracorporeal immunoadsorption (ECIA) is a new method for the selective removal of circulating radiolabeled monoclonal antibodies (MAb) from plasma to increase the uptake in tumor versus normal tissues (T/N-ratio). To ascertain whether the amount of MAb affects T/N ratios immediately and 24 h after ECIA, we used a rat model with two tumor sites--one intramuscular (im) and one below the subrenal capsule (SR). Extracorporeal immunoadsorption was done with an avidin-agarose column after injection of 125I-labeled biotinylated L6 MAb. The animals received 10, 50 or 250 micrograms of L6 only (controls), or followed by ECIA. The efficacy of the procedure in removing plasma activity was 80-95%. For both tumor sites, the highest T/N-ratios were obtained with 10 micrograms L6. All T/N-ratios significantly improved for SR tumors by a factor ranging from 3.2 (lung) to 12.6 (bone marrow). The T/N-ratios were still elevated 24 h after ECIA. Injection of larger amounts of MAb, probably causing a higher degree of tumor saturation, will not necessarily improve the T/N ratio after ECIA.},
  author       = {Garkavij, Michael and Tennvall, Jan and Strand, Sven-Erik and Norrgren, Kristina and Nilsson, Rune and Lindgren, Lars and Sjögren, Hans Olov},
  issn         = {1651-226X},
  language     = {eng},
  number       = {7-8},
  pages        = {853--859},
  publisher    = {Taylor & Francis},
  series       = {Acta Oncologica},
  title        = {Improving radioimmonotargeting of tumors. Variation in the amount of L6 MAb administered, combined with an immunoadsorption system (ECIA)},
  url          = {http://dx.doi.org/10.3109/02841869309096146},
  volume       = {32},
  year         = {1993},
}