The influence of membrane potential on chloride channels activated by GABA in rat cultured hippocampal neurons
(1993) In Journal of Membrane Biology 136(3). p.273-280- Abstract
- Chloride currents were activated by a low concentration of GABA (0.5 microM) in neonatal rat hippocampal neurons cultured for up to 14 days. Currents elicited by 0.5 microM GABA in neurons, voltage-clamped using the whole-cell technique with pipettes containing 149 mM Cl-, reversed close to 0 mV whether pipettes contained 144 mM Na+ or 140 mM Cs+, and were blocked by 100 microM bicuculline. Current-voltage curves showed outward rectification. Single channel currents appeared in cell-attached patches when the pipette tip was perfused with pipette solution containing 0.5 microM GABA and disappeared when a solution containing 100 microM bicuculline plus 0.5 microM GABA was injected into the pipette tip. The channels showed outward... (More)
- Chloride currents were activated by a low concentration of GABA (0.5 microM) in neonatal rat hippocampal neurons cultured for up to 14 days. Currents elicited by 0.5 microM GABA in neurons, voltage-clamped using the whole-cell technique with pipettes containing 149 mM Cl-, reversed close to 0 mV whether pipettes contained 144 mM Na+ or 140 mM Cs+, and were blocked by 100 microM bicuculline. Current-voltage curves showed outward rectification. Single channel currents appeared in cell-attached patches when the pipette tip was perfused with pipette solution containing 0.5 microM GABA and disappeared when a solution containing 100 microM bicuculline plus 0.5 microM GABA was injected into the pipette tip. The channels showed outward rectification and, in some patches, had a much lower probability of opening at hyperpolarized potentials. The average chord conductance in 10 patches hyperpolarized by 80 mV was 7.8 +/- 1.6 pS (SEM) compared with a chord conductance of 34.1 +/- 3.5 pS (SEM) in the same patches depolarized by 80 mV. Similar single channel currents were also activated in cell-free, inside-out patches in symmetrical chloride solutions when 0.5 microM GABA was injected into the pipette tip. The channels showed outward rectification similar to that seen in cell-attached patches, and some channels had a lower probability of opening at hyperpolarized potentials. The average chord conductance in 13 patches hyperpolarized by 80 mV was 11.8 +/- 2.3 pS (SEM) compared with 42.1 +/- 3.1 pS (SEM) in the same patches depolarized by 80 mV. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1107178
- author
- Curmi, J P ; Premkumar, L S ; Birnir, Bryndis LU and Gage, P W
- publishing date
- 1993
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- GABA, Chloride channels, Outward rectification, Hippocampus
- in
- Journal of Membrane Biology
- volume
- 136
- issue
- 3
- pages
- 273 - 280
- publisher
- Springer
- external identifiers
-
- pmid:8114077
- scopus:0027134917
- ISSN
- 0022-2631
- DOI
- 10.1007/BF00233666
- language
- English
- LU publication?
- no
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: GABA Channels in Physiology and Pharmacology (013241570)
- id
- 5ba8dfa4-de1f-46bc-9a13-9fcbd25991d2 (old id 1107178)
- date added to LUP
- 2016-04-01 16:33:57
- date last changed
- 2021-01-03 10:15:31
@article{5ba8dfa4-de1f-46bc-9a13-9fcbd25991d2,
abstract = {{Chloride currents were activated by a low concentration of GABA (0.5 microM) in neonatal rat hippocampal neurons cultured for up to 14 days. Currents elicited by 0.5 microM GABA in neurons, voltage-clamped using the whole-cell technique with pipettes containing 149 mM Cl-, reversed close to 0 mV whether pipettes contained 144 mM Na+ or 140 mM Cs+, and were blocked by 100 microM bicuculline. Current-voltage curves showed outward rectification. Single channel currents appeared in cell-attached patches when the pipette tip was perfused with pipette solution containing 0.5 microM GABA and disappeared when a solution containing 100 microM bicuculline plus 0.5 microM GABA was injected into the pipette tip. The channels showed outward rectification and, in some patches, had a much lower probability of opening at hyperpolarized potentials. The average chord conductance in 10 patches hyperpolarized by 80 mV was 7.8 +/- 1.6 pS (SEM) compared with a chord conductance of 34.1 +/- 3.5 pS (SEM) in the same patches depolarized by 80 mV. Similar single channel currents were also activated in cell-free, inside-out patches in symmetrical chloride solutions when 0.5 microM GABA was injected into the pipette tip. The channels showed outward rectification similar to that seen in cell-attached patches, and some channels had a lower probability of opening at hyperpolarized potentials. The average chord conductance in 13 patches hyperpolarized by 80 mV was 11.8 +/- 2.3 pS (SEM) compared with 42.1 +/- 3.1 pS (SEM) in the same patches depolarized by 80 mV.}},
author = {{Curmi, J P and Premkumar, L S and Birnir, Bryndis and Gage, P W}},
issn = {{0022-2631}},
keywords = {{GABA; Chloride channels; Outward rectification; Hippocampus}},
language = {{eng}},
number = {{3}},
pages = {{273--280}},
publisher = {{Springer}},
series = {{Journal of Membrane Biology}},
title = {{The influence of membrane potential on chloride channels activated by GABA in rat cultured hippocampal neurons}},
url = {{http://dx.doi.org/10.1007/BF00233666}},
doi = {{10.1007/BF00233666}},
volume = {{136}},
year = {{1993}},
}