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Non-adrenergic, non-cholinergic neural activation in guinea-pig bronchi: powerful and frequency-dependent stabilizing effect on tone

Linden, A; Ullman, A; Löfdahl, Claes-Göran LU and Skoogh, B E (1993) In British Journal of Pharmacology 109(3). p.845-851
Abstract
1 We examined non-adrenergic, non-cholinergic (NANC) stimulation for its stabilizing effect on bronchial smooth-muscle tone with respect to its regulatory power and the effect of variations in neural impulse frequency.

2 The guinea-pig isolated main bronchus (n = 4-12) was pretreated with indomethacin (1O JAM) and incubated with atropine (1 pM) and guanethidine (10 JM). Electrical field stimulation (EFS: 1200 mA, 0.5 ms, 240 s) was applied at various levels of tone prior to EFS: first without tone, then at a moderate tone induced by histamine (0.3tJM) and, finally, at a high tone induced by histamine (611M). Three

different stimulation frequencies (1, 3 or 10Hz) were used in order to produce moderate to nearmaximum... (More)
1 We examined non-adrenergic, non-cholinergic (NANC) stimulation for its stabilizing effect on bronchial smooth-muscle tone with respect to its regulatory power and the effect of variations in neural impulse frequency.

2 The guinea-pig isolated main bronchus (n = 4-12) was pretreated with indomethacin (1O JAM) and incubated with atropine (1 pM) and guanethidine (10 JM). Electrical field stimulation (EFS: 1200 mA, 0.5 ms, 240 s) was applied at various levels of tone prior to EFS: first without tone, then at a moderate tone induced by histamine (0.3tJM) and, finally, at a high tone induced by histamine (611M). Three

different stimulation frequencies (1, 3 or 10Hz) were used in order to produce moderate to nearmaximum contractile and relaxant NANC neural responses. Both the contractile and the relaxant NANC responses were tetrodotoxin-sensitive in the guinea-pig isolated main bronchus (3 Hz).

3 Without tone prior to EFS, NANC activation (1, 3 or 10Hz) induced a pronounced contractile response. At a moderate level of tone prior to EFS, NANC activation induced a less pronounced contractile response. At the highest level of tone prior to EFS, NANC activation induced a relaxant

response. All these NANC responses adjusted the tone towards a similar level and this 'stabilization level' was 56(6)% at 1 Hz, 65(3)% at 3 Hz and 56(5)% at 10 Hz, expressed as a percentage of the maximum histamine-induced (0.1 mM) tone in each airway preparation.

4 There was a difference of approximately 90% of maximum between the highest and the lowest tone level prior to NANC activation. This difference was reduced by the converging contractile and relaxant NANC responses and the magnitude of this 'convergence effect' was 40(8)% at 1 Hz, 72(4)% at 3 Hz and 90(2)% at 10 Hz.

5 These findings indicate that NANC neural activation stabilizes bronchial smooth-muscle tone via a contraction when the tone is low prior to activation and via a relaxation when the tone is high prior to activation. The NANC stabilizing effect on tone appears to be powerful and its magnitude can be controlled by the neural impulse frequency. The level of tone towards which the NANC responses converge does not appear to be markedly altered by variations in the impulse frequency. Our findings

are consistent with a regulatory role for NANC responses in the control of bronchial smooth-muscle tone. (Less)
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author
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Airway smooth muscle, guinea-pig bronchi, in vitro, neural regulation, non-adrenergic, non-cholinergic, tone
in
British Journal of Pharmacology
volume
109
issue
3
pages
845 - 851
publisher
The British Pharmacological Society
external identifiers
  • pmid:8358575
  • scopus:0027318697
ISSN
1476-5381
language
English
LU publication?
no
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712aaa83-f6a8-43b0-a3fd-224cac1a1b50 (old id 1107409)
alternative location
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2175637
date added to LUP
2008-07-31 08:45:01
date last changed
2017-01-01 07:07:00
@article{712aaa83-f6a8-43b0-a3fd-224cac1a1b50,
  abstract     = {1 We examined non-adrenergic, non-cholinergic (NANC) stimulation for its stabilizing effect on bronchial smooth-muscle tone with respect to its regulatory power and the effect of variations in neural impulse frequency.<br/><br>
2 The guinea-pig isolated main bronchus (n = 4-12) was pretreated with indomethacin (1O JAM) and incubated with atropine (1 pM) and guanethidine (10 JM). Electrical field stimulation (EFS: 1200 mA, 0.5 ms, 240 s) was applied at various levels of tone prior to EFS: first without tone, then at a moderate tone induced by histamine (0.3tJM) and, finally, at a high tone induced by histamine (611M). Three<br/><br>
different stimulation frequencies (1, 3 or 10Hz) were used in order to produce moderate to nearmaximum contractile and relaxant NANC neural responses. Both the contractile and the relaxant NANC responses were tetrodotoxin-sensitive in the guinea-pig isolated main bronchus (3 Hz).<br/><br>
3 Without tone prior to EFS, NANC activation (1, 3 or 10Hz) induced a pronounced contractile response. At a moderate level of tone prior to EFS, NANC activation induced a less pronounced contractile response. At the highest level of tone prior to EFS, NANC activation induced a relaxant<br/><br>
response. All these NANC responses adjusted the tone towards a similar level and this 'stabilization level' was 56(6)% at 1 Hz, 65(3)% at 3 Hz and 56(5)% at 10 Hz, expressed as a percentage of the maximum histamine-induced (0.1 mM) tone in each airway preparation.<br/><br>
4 There was a difference of approximately 90% of maximum between the highest and the lowest tone level prior to NANC activation. This difference was reduced by the converging contractile and relaxant NANC responses and the magnitude of this 'convergence effect' was 40(8)% at 1 Hz, 72(4)% at 3 Hz and 90(2)% at 10 Hz.<br/><br>
5 These findings indicate that NANC neural activation stabilizes bronchial smooth-muscle tone via a contraction when the tone is low prior to activation and via a relaxation when the tone is high prior to activation. The NANC stabilizing effect on tone appears to be powerful and its magnitude can be controlled by the neural impulse frequency. The level of tone towards which the NANC responses converge does not appear to be markedly altered by variations in the impulse frequency. Our findings<br/><br>
are consistent with a regulatory role for NANC responses in the control of bronchial smooth-muscle tone.},
  author       = {Linden, A and Ullman, A and Löfdahl, Claes-Göran and Skoogh, B E},
  issn         = {1476-5381},
  keyword      = {Airway smooth muscle,guinea-pig bronchi,in vitro,neural regulation,non-adrenergic,non-cholinergic,tone},
  language     = {eng},
  number       = {3},
  pages        = {845--851},
  publisher    = {The British Pharmacological Society},
  series       = {British Journal of Pharmacology},
  title        = {Non-adrenergic, non-cholinergic neural activation in guinea-pig bronchi: powerful and frequency-dependent stabilizing effect on tone},
  volume       = {109},
  year         = {1993},
}