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Protein secretion from mouse skeletal muscle: coupling of increased exocytotic and endocytotic activities in denervated muscle

Vult von Steyern, Fredrik LU ; Kanje, Martin LU and Tågerud, S (1993) In Cell and Tissue Research 274(1). p.49-56
Abstract
The secretion of proteins labelled by incorporation of radioactive amino acids was studied in innervated and 10 to 13-day-denervated mouse skeletal muscle. The secretion of 3H-leucine-labelled proteins, expressed per mg muscle wet weight, increased after denervation, and the kinetics of the secretory process was also altered in denervated muscle. Separation of secreted 35S-methionine-labelled proteins by sodium dodecyl sulphate polyacrylamide gel electrophoresis followed by autoradiography revealed some denervation-induced alterations in the pattern of secreted proteins. The secretion from both innervated and denervated muscle was highly temperature sensitive and was reversibly inhibited by brefeldin A, a drug that blocks forward membrane... (More)
The secretion of proteins labelled by incorporation of radioactive amino acids was studied in innervated and 10 to 13-day-denervated mouse skeletal muscle. The secretion of 3H-leucine-labelled proteins, expressed per mg muscle wet weight, increased after denervation, and the kinetics of the secretory process was also altered in denervated muscle. Separation of secreted 35S-methionine-labelled proteins by sodium dodecyl sulphate polyacrylamide gel electrophoresis followed by autoradiography revealed some denervation-induced alterations in the pattern of secreted proteins. The secretion from both innervated and denervated muscle was highly temperature sensitive and was reversibly inhibited by brefeldin A, a drug that blocks forward membrane transport from the endoplasmic reticulum/Golgi apparatus. This drug was also found to inhibit the uptake of fluorescein isothiocyanate-labelled dextran in denervated muscle but had no effect on the endocytotic activity of innervated muscle. This lends support to the hypothesis that the increased endocytotic activity in denervated muscle is coupled to a high secretory activity. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Muscle, skeletal, Exocytosis, Endocytosis, Denervation, Brefeldin A, Mouse (NMRI)
in
Cell and Tissue Research
volume
274
issue
1
pages
49 - 56
publisher
Springer
external identifiers
  • pmid:7694802
  • scopus:0027370143
ISSN
1432-0878
DOI
10.1007/BF00327984
language
English
LU publication?
yes
id
8efc32de-c572-4185-88b8-d77ef5abd329 (old id 1107528)
date added to LUP
2008-07-31 10:54:36
date last changed
2017-08-27 04:07:54
@article{8efc32de-c572-4185-88b8-d77ef5abd329,
  abstract     = {The secretion of proteins labelled by incorporation of radioactive amino acids was studied in innervated and 10 to 13-day-denervated mouse skeletal muscle. The secretion of 3H-leucine-labelled proteins, expressed per mg muscle wet weight, increased after denervation, and the kinetics of the secretory process was also altered in denervated muscle. Separation of secreted 35S-methionine-labelled proteins by sodium dodecyl sulphate polyacrylamide gel electrophoresis followed by autoradiography revealed some denervation-induced alterations in the pattern of secreted proteins. The secretion from both innervated and denervated muscle was highly temperature sensitive and was reversibly inhibited by brefeldin A, a drug that blocks forward membrane transport from the endoplasmic reticulum/Golgi apparatus. This drug was also found to inhibit the uptake of fluorescein isothiocyanate-labelled dextran in denervated muscle but had no effect on the endocytotic activity of innervated muscle. This lends support to the hypothesis that the increased endocytotic activity in denervated muscle is coupled to a high secretory activity.},
  author       = {Vult von Steyern, Fredrik and Kanje, Martin and Tågerud, S},
  issn         = {1432-0878},
  keyword      = {Muscle,skeletal,Exocytosis,Endocytosis,Denervation,Brefeldin A,Mouse (NMRI)},
  language     = {eng},
  number       = {1},
  pages        = {49--56},
  publisher    = {Springer},
  series       = {Cell and Tissue Research},
  title        = {Protein secretion from mouse skeletal muscle: coupling of increased exocytotic and endocytotic activities in denervated muscle},
  url          = {http://dx.doi.org/10.1007/BF00327984},
  volume       = {274},
  year         = {1993},
}