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Effects of nerve growth factor on X-irradiated reaggregation cultures of rat brain cells

Dimberg, Y; Aspberg, Anders LU and Tottmar, O (1993) In International Journal of Radiation Biology 64(6). p.731-737
Abstract
The effects of exogenously added nerve growth factor (NGF) on reaggregation cultures of foetal rat brain cells after X-irradiation with 2 Gy were studied. Irradiation caused decreased protein and DNA levels, which was not prevented by NGF. The activities of the cholinergic marker enzymes choline acetyl transferase and acetylcholine esterase were increased in irradiated cultures. However, no difference in the activities of these enzymes was found between irradiated and unirradiated NGF-treated cultures. Irradiation did not affect the activity of the marker enzyme for oligodendrocytes (2',3'-cyclic nucleotide 3'-phosphodiesterase), but caused an increase in the astrocyte marker (glutamine synthetase) activity. This effect on astrocytes was... (More)
The effects of exogenously added nerve growth factor (NGF) on reaggregation cultures of foetal rat brain cells after X-irradiation with 2 Gy were studied. Irradiation caused decreased protein and DNA levels, which was not prevented by NGF. The activities of the cholinergic marker enzymes choline acetyl transferase and acetylcholine esterase were increased in irradiated cultures. However, no difference in the activities of these enzymes was found between irradiated and unirradiated NGF-treated cultures. Irradiation did not affect the activity of the marker enzyme for oligodendrocytes (2',3'-cyclic nucleotide 3'-phosphodiesterase), but caused an increase in the astrocyte marker (glutamine synthetase) activity. This effect on astrocytes was prevented by NGF. (Less)
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author
publishing date
type
Contribution to journal
publication status
published
subject
in
International Journal of Radiation Biology
volume
64
issue
6
pages
731 - 737
publisher
Taylor & Francis
external identifiers
  • pmid:7903341
  • scopus:0027135272
ISSN
0955-3002
DOI
10.1080/09553009314551981
language
English
LU publication?
no
id
7ff09b7c-8843-400c-b0a2-33d46c660870 (old id 1107660)
date added to LUP
2008-07-31 14:22:03
date last changed
2017-01-01 05:12:08
@article{7ff09b7c-8843-400c-b0a2-33d46c660870,
  abstract     = {The effects of exogenously added nerve growth factor (NGF) on reaggregation cultures of foetal rat brain cells after X-irradiation with 2 Gy were studied. Irradiation caused decreased protein and DNA levels, which was not prevented by NGF. The activities of the cholinergic marker enzymes choline acetyl transferase and acetylcholine esterase were increased in irradiated cultures. However, no difference in the activities of these enzymes was found between irradiated and unirradiated NGF-treated cultures. Irradiation did not affect the activity of the marker enzyme for oligodendrocytes (2',3'-cyclic nucleotide 3'-phosphodiesterase), but caused an increase in the astrocyte marker (glutamine synthetase) activity. This effect on astrocytes was prevented by NGF.},
  author       = {Dimberg, Y and Aspberg, Anders and Tottmar, O},
  issn         = {0955-3002},
  language     = {eng},
  number       = {6},
  pages        = {731--737},
  publisher    = {Taylor & Francis},
  series       = {International Journal of Radiation Biology},
  title        = {Effects of nerve growth factor on X-irradiated reaggregation cultures of rat brain cells},
  url          = {http://dx.doi.org/10.1080/09553009314551981},
  volume       = {64},
  year         = {1993},
}