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Clinical impact of breakpoint position within M-bcr in chronic myeloid leukemia

Fioretos, Thoas LU ; Nilsson, Per-Gunnar LU ; Åman, P; Heim, Sverre LU ; Kristoffersson, Ulf LU ; Malm, C; Simonsson, B; Turesson, Ingemar LU and Mitelman, Felix LU (1993) In Leukemia 7(8). p.1225-1231
Abstract
We have analyzed the M-bcr breakpoint position in 133 Philadelphia-positive chronic myeloid leukemia patients and correlated the findings with clinical, hematologic, and cytogenetic data. We also investigated the splicing pattern of the BCR-ABL mRNA in 30 patients, using reverse transcriptase PCR. No statistically significant differences were found between breakpoint position within M-bcr and clinical parameters at diagnosis, the karyotypic evolution pattern, or the leukemic phenotype during blast crisis. Furthermore, the breakpoint position within M-bcr did not correlate with the duration of chronic phase or survival time. When the splicing pattern of the BCR-ABL mRNA was compared with the results of the genomic breakpoint mapping, it was... (More)
We have analyzed the M-bcr breakpoint position in 133 Philadelphia-positive chronic myeloid leukemia patients and correlated the findings with clinical, hematologic, and cytogenetic data. We also investigated the splicing pattern of the BCR-ABL mRNA in 30 patients, using reverse transcriptase PCR. No statistically significant differences were found between breakpoint position within M-bcr and clinical parameters at diagnosis, the karyotypic evolution pattern, or the leukemic phenotype during blast crisis. Furthermore, the breakpoint position within M-bcr did not correlate with the duration of chronic phase or survival time. When the splicing pattern of the BCR-ABL mRNA was compared with the results of the genomic breakpoint mapping, it was found that approximately 60% (8/14) of the patients with a 5' break expressed b2a2 fusion mRNA, whereas all patients (10/10) with a 3' break expressed b3a2 BCR-ABL mRNA. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Leukemia
volume
7
issue
8
pages
1225 - 1231
publisher
Nature Publishing Group
external identifiers
  • pmid:8350622
  • scopus:0027236472
ISSN
1476-5551
language
English
LU publication?
yes
id
1d4d4d59-3064-4a06-9f9f-9161702a587f (old id 1107680)
date added to LUP
2008-07-31 14:53:02
date last changed
2017-01-01 06:45:46
@article{1d4d4d59-3064-4a06-9f9f-9161702a587f,
  abstract     = {We have analyzed the M-bcr breakpoint position in 133 Philadelphia-positive chronic myeloid leukemia patients and correlated the findings with clinical, hematologic, and cytogenetic data. We also investigated the splicing pattern of the BCR-ABL mRNA in 30 patients, using reverse transcriptase PCR. No statistically significant differences were found between breakpoint position within M-bcr and clinical parameters at diagnosis, the karyotypic evolution pattern, or the leukemic phenotype during blast crisis. Furthermore, the breakpoint position within M-bcr did not correlate with the duration of chronic phase or survival time. When the splicing pattern of the BCR-ABL mRNA was compared with the results of the genomic breakpoint mapping, it was found that approximately 60% (8/14) of the patients with a 5' break expressed b2a2 fusion mRNA, whereas all patients (10/10) with a 3' break expressed b3a2 BCR-ABL mRNA.},
  author       = {Fioretos, Thoas and Nilsson, Per-Gunnar and Åman, P and Heim, Sverre and Kristoffersson, Ulf and Malm, C and Simonsson, B and Turesson, Ingemar and Mitelman, Felix},
  issn         = {1476-5551},
  language     = {eng},
  number       = {8},
  pages        = {1225--1231},
  publisher    = {Nature Publishing Group},
  series       = {Leukemia},
  title        = {Clinical impact of breakpoint position within M-bcr in chronic myeloid leukemia},
  volume       = {7},
  year         = {1993},
}