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Inter-institutional reproducibility of flow cytometric DNA-analysis in breast carcinomas

Risberg, B; Baldetorp, Bo LU ; Fernö, Mårten LU ; Hansson, B; Mossberg, L L; Roos, G; Stal, O; Thornthwaite, J T and Tibell, L (1994) In Analytical Cellular Pathology 6(1). p.23-36
Abstract
In order to study interinstitutional reproducibility of flow cytometric DNA-analysis (DNA-FCM), frozen pieces from 30 consecutive breast carcinomas were analysed by 5 laboratories. Different instruments, preparation and DNA staining methods were used. A concordance in DNA-ploidy status was obtained in 26 of the 30 tumours. The discrepancy can mainly be explained by intratumoural DNA-heterogeneity since a complete agreement in ploidy status was obtained when four of the laboratories analysed the same cell suspension, where solid bits showed differing results. The sampling method seems therefore to be a crucial step for the results and needs further studies. As far as the estimation of S-phase fraction was concerned, one laboratory obtained... (More)
In order to study interinstitutional reproducibility of flow cytometric DNA-analysis (DNA-FCM), frozen pieces from 30 consecutive breast carcinomas were analysed by 5 laboratories. Different instruments, preparation and DNA staining methods were used. A concordance in DNA-ploidy status was obtained in 26 of the 30 tumours. The discrepancy can mainly be explained by intratumoural DNA-heterogeneity since a complete agreement in ploidy status was obtained when four of the laboratories analysed the same cell suspension, where solid bits showed differing results. The sampling method seems therefore to be a crucial step for the results and needs further studies. As far as the estimation of S-phase fraction was concerned, one laboratory obtained significantly higher values compared to the other four. The correlation between the other four laboratories varied between r = 0.66-0.92. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Analytical Cellular Pathology
volume
6
issue
1
pages
23 - 36
publisher
IOS Press
external identifiers
  • pmid:8130128
  • scopus:0027972485
ISSN
0921-8912
language
English
LU publication?
yes
id
fa641683-613b-42d8-a9d0-0a5c552130e3 (old id 1107804)
date added to LUP
2008-07-22 15:36:03
date last changed
2017-01-01 06:42:23
@article{fa641683-613b-42d8-a9d0-0a5c552130e3,
  abstract     = {In order to study interinstitutional reproducibility of flow cytometric DNA-analysis (DNA-FCM), frozen pieces from 30 consecutive breast carcinomas were analysed by 5 laboratories. Different instruments, preparation and DNA staining methods were used. A concordance in DNA-ploidy status was obtained in 26 of the 30 tumours. The discrepancy can mainly be explained by intratumoural DNA-heterogeneity since a complete agreement in ploidy status was obtained when four of the laboratories analysed the same cell suspension, where solid bits showed differing results. The sampling method seems therefore to be a crucial step for the results and needs further studies. As far as the estimation of S-phase fraction was concerned, one laboratory obtained significantly higher values compared to the other four. The correlation between the other four laboratories varied between r = 0.66-0.92.},
  author       = {Risberg, B and Baldetorp, Bo and Fernö, Mårten and Hansson, B and Mossberg, L L and Roos, G and Stal, O and Thornthwaite, J T and Tibell, L},
  issn         = {0921-8912},
  language     = {eng},
  number       = {1},
  pages        = {23--36},
  publisher    = {IOS Press},
  series       = {Analytical Cellular Pathology},
  title        = {Inter-institutional reproducibility of flow cytometric DNA-analysis in breast carcinomas},
  volume       = {6},
  year         = {1994},
}