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A general extracorporeal immunoadsorption method to increase tumor-to-tissue ratio

Strand, Sven-Erik LU ; Norrgren, Kristina LU ; Garkavij, Michael LU ; Lindgren, L ; Nilsson, Rune LU ; Sjogren, H O and Tennvall, Jan LU (1994) In Cancer 73(3 Suppl). p.1033-1037
Abstract
The idea of applying extracorporeal immunoadsorption (ECIA) in radioimmunodiagnosis and radioimmunotherapy has been proposed previously. The authors here report on the development of new concept using a general method for ECIA based on biotinylated MoAb adsorbed on an avidin column. Athymic rats heterotransplanted with either human melanomas or human lung carcinoma were injected with iodine-125-labeled biotinylated 96.5 or L6 MoAb, respectively. At 24 or 48 hours after the injection, ECIA was performed by pumping blood through a hollow-fiber plasma filter. The separated plasma then was passed through an absorbent (avidin-agarose) column. The whole ECIA procedure lasted for 3 hours. By this ECIA method, the tumor-to-normal tissue ratios... (More)
The idea of applying extracorporeal immunoadsorption (ECIA) in radioimmunodiagnosis and radioimmunotherapy has been proposed previously. The authors here report on the development of new concept using a general method for ECIA based on biotinylated MoAb adsorbed on an avidin column. Athymic rats heterotransplanted with either human melanomas or human lung carcinoma were injected with iodine-125-labeled biotinylated 96.5 or L6 MoAb, respectively. At 24 or 48 hours after the injection, ECIA was performed by pumping blood through a hollow-fiber plasma filter. The separated plasma then was passed through an absorbent (avidin-agarose) column. The whole ECIA procedure lasted for 3 hours. By this ECIA method, the tumor-to-normal tissue ratios were increased in various tissues (i.e., radiosensitive and blood rich organs) by a factor of four to five. (Less)
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author
; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Cancer
volume
73
issue
3 Suppl
pages
1033 - 1037
publisher
John Wiley & Sons Inc.
external identifiers
  • pmid:8306245
  • scopus:0028125842
ISSN
1097-0142
language
English
LU publication?
yes
id
9bd788d6-42ef-42d2-8cc2-5ee23905d022 (old id 1107982)
date added to LUP
2016-04-01 12:23:41
date last changed
2021-01-03 06:36:09
@article{9bd788d6-42ef-42d2-8cc2-5ee23905d022,
  abstract     = {{The idea of applying extracorporeal immunoadsorption (ECIA) in radioimmunodiagnosis and radioimmunotherapy has been proposed previously. The authors here report on the development of new concept using a general method for ECIA based on biotinylated MoAb adsorbed on an avidin column. Athymic rats heterotransplanted with either human melanomas or human lung carcinoma were injected with iodine-125-labeled biotinylated 96.5 or L6 MoAb, respectively. At 24 or 48 hours after the injection, ECIA was performed by pumping blood through a hollow-fiber plasma filter. The separated plasma then was passed through an absorbent (avidin-agarose) column. The whole ECIA procedure lasted for 3 hours. By this ECIA method, the tumor-to-normal tissue ratios were increased in various tissues (i.e., radiosensitive and blood rich organs) by a factor of four to five.}},
  author       = {{Strand, Sven-Erik and Norrgren, Kristina and Garkavij, Michael and Lindgren, L and Nilsson, Rune and Sjogren, H O and Tennvall, Jan}},
  issn         = {{1097-0142}},
  language     = {{eng}},
  number       = {{3 Suppl}},
  pages        = {{1033--1037}},
  publisher    = {{John Wiley & Sons Inc.}},
  series       = {{Cancer}},
  title        = {{A general extracorporeal immunoadsorption method to increase tumor-to-tissue ratio}},
  volume       = {{73}},
  year         = {{1994}},
}