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A general extracorporeal immunoadsorption method to increase tumor-to-tissue ratio

Strand, Sven-Erik LU ; Norrgren, Kristina LU ; Garkavij, Michael LU ; Lindgren, L; Nilsson, Rune LU ; Sjogren, H O and Tennvall, Jan LU (1994) In Cancer 73(3 Suppl). p.1033-1037
Abstract
The idea of applying extracorporeal immunoadsorption (ECIA) in radioimmunodiagnosis and radioimmunotherapy has been proposed previously. The authors here report on the development of new concept using a general method for ECIA based on biotinylated MoAb adsorbed on an avidin column. Athymic rats heterotransplanted with either human melanomas or human lung carcinoma were injected with iodine-125-labeled biotinylated 96.5 or L6 MoAb, respectively. At 24 or 48 hours after the injection, ECIA was performed by pumping blood through a hollow-fiber plasma filter. The separated plasma then was passed through an absorbent (avidin-agarose) column. The whole ECIA procedure lasted for 3 hours. By this ECIA method, the tumor-to-normal tissue ratios... (More)
The idea of applying extracorporeal immunoadsorption (ECIA) in radioimmunodiagnosis and radioimmunotherapy has been proposed previously. The authors here report on the development of new concept using a general method for ECIA based on biotinylated MoAb adsorbed on an avidin column. Athymic rats heterotransplanted with either human melanomas or human lung carcinoma were injected with iodine-125-labeled biotinylated 96.5 or L6 MoAb, respectively. At 24 or 48 hours after the injection, ECIA was performed by pumping blood through a hollow-fiber plasma filter. The separated plasma then was passed through an absorbent (avidin-agarose) column. The whole ECIA procedure lasted for 3 hours. By this ECIA method, the tumor-to-normal tissue ratios were increased in various tissues (i.e., radiosensitive and blood rich organs) by a factor of four to five. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Cancer
volume
73
issue
3 Suppl
pages
1033 - 1037
publisher
John Wiley & Sons
external identifiers
  • pmid:8306245
  • scopus:0028125842
ISSN
1097-0142
language
English
LU publication?
yes
id
9bd788d6-42ef-42d2-8cc2-5ee23905d022 (old id 1107982)
date added to LUP
2008-07-23 10:43:29
date last changed
2017-01-01 05:06:21
@article{9bd788d6-42ef-42d2-8cc2-5ee23905d022,
  abstract     = {The idea of applying extracorporeal immunoadsorption (ECIA) in radioimmunodiagnosis and radioimmunotherapy has been proposed previously. The authors here report on the development of new concept using a general method for ECIA based on biotinylated MoAb adsorbed on an avidin column. Athymic rats heterotransplanted with either human melanomas or human lung carcinoma were injected with iodine-125-labeled biotinylated 96.5 or L6 MoAb, respectively. At 24 or 48 hours after the injection, ECIA was performed by pumping blood through a hollow-fiber plasma filter. The separated plasma then was passed through an absorbent (avidin-agarose) column. The whole ECIA procedure lasted for 3 hours. By this ECIA method, the tumor-to-normal tissue ratios were increased in various tissues (i.e., radiosensitive and blood rich organs) by a factor of four to five.},
  author       = {Strand, Sven-Erik and Norrgren, Kristina and Garkavij, Michael and Lindgren, L and Nilsson, Rune and Sjogren, H O and Tennvall, Jan},
  issn         = {1097-0142},
  language     = {eng},
  number       = {3 Suppl},
  pages        = {1033--1037},
  publisher    = {John Wiley & Sons},
  series       = {Cancer},
  title        = {A general extracorporeal immunoadsorption method to increase tumor-to-tissue ratio},
  volume       = {73},
  year         = {1994},
}