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Effect of maturation on airway plasma exudation induced by eicosanoids in guinea pig

Arakawa, H ; Lotvall, J ; Kawikova, I ; Tokuyama, K ; Löfdahl, Claes-Göran LU and Skoogh, B E (1994) In European Journal of Pharmacology 259(3). p.251-257
Abstract
Airway reactivity to bronchoconstrictor mediators changes with age. We studied the effects of maturational change on airway responses induced by a thromboxane A2 mimetic, U-46619 (2, 6 and 20 nmol/kg; i.v.), leukotriene D4 (0.6 and 2 nmol/kg; i.v.) or vehicle (0.9% NaCl; i.v.) in immature (196 +/- 3 g: 2 weeks) and adult guinea pigs (512 +/- 5 g: 11 weeks). In the same animals, we measured both lung resistance (RL) to monitor airflow obstruction and extravasation of Evans Blue dye (20 mg/kg) to quantify airway plasma exudation. For a comparison, changes in RL in response to acetylcholine (5, 15 and 50 nmol/kg; i.v.) were also examined in both age groups. The order of potency to induce an increase in RL did not change with age (leukotriene... (More)
Airway reactivity to bronchoconstrictor mediators changes with age. We studied the effects of maturational change on airway responses induced by a thromboxane A2 mimetic, U-46619 (2, 6 and 20 nmol/kg; i.v.), leukotriene D4 (0.6 and 2 nmol/kg; i.v.) or vehicle (0.9% NaCl; i.v.) in immature (196 +/- 3 g: 2 weeks) and adult guinea pigs (512 +/- 5 g: 11 weeks). In the same animals, we measured both lung resistance (RL) to monitor airflow obstruction and extravasation of Evans Blue dye (20 mg/kg) to quantify airway plasma exudation. For a comparison, changes in RL in response to acetylcholine (5, 15 and 50 nmol/kg; i.v.) were also examined in both age groups. The order of potency to induce an increase in RL did not change with age (leukotriene D4 > U-46619 > acetylcholine). In immature animals, the peak RL after U-46619 (2, 6 and 20 nmol/kg; P < 0.05, P < 0.005 and P < 0.01, respectively) and leukotriene D4 (2 nmol/kg; P < 0.01) was significantly higher than in adult animals. U-46619 and leukotriene D4 produced significant extravasation of Evans Blue dye in both immature and adult animals. The order of potency to induce extravasated dye also did not change with age (leukotriene D4 > U-46619). The amount of extravasation of Evans Blue dye after U-46619 (6 and 20 nmol/kg) and leukotriene D4 (0.6 and 2 nmol/kg) was significantly smaller in immature animals than adults at all airway levels.(ABSTRACT TRUNCATED AT 250 WORDS) (Less)
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organization
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Contribution to journal
publication status
published
subject
in
European Journal of Pharmacology
volume
259
issue
3
pages
251 - 257
publisher
Elsevier
external identifiers
  • pmid:7982451
  • scopus:0028319039
ISSN
1879-0712
language
English
LU publication?
yes
id
a72f66c3-3454-4b24-86a3-63991ff442bc (old id 1108301)
date added to LUP
2016-04-01 12:29:44
date last changed
2021-01-03 08:15:30
@article{a72f66c3-3454-4b24-86a3-63991ff442bc,
  abstract     = {{Airway reactivity to bronchoconstrictor mediators changes with age. We studied the effects of maturational change on airway responses induced by a thromboxane A2 mimetic, U-46619 (2, 6 and 20 nmol/kg; i.v.), leukotriene D4 (0.6 and 2 nmol/kg; i.v.) or vehicle (0.9% NaCl; i.v.) in immature (196 +/- 3 g: 2 weeks) and adult guinea pigs (512 +/- 5 g: 11 weeks). In the same animals, we measured both lung resistance (RL) to monitor airflow obstruction and extravasation of Evans Blue dye (20 mg/kg) to quantify airway plasma exudation. For a comparison, changes in RL in response to acetylcholine (5, 15 and 50 nmol/kg; i.v.) were also examined in both age groups. The order of potency to induce an increase in RL did not change with age (leukotriene D4 &gt; U-46619 &gt; acetylcholine). In immature animals, the peak RL after U-46619 (2, 6 and 20 nmol/kg; P &lt; 0.05, P &lt; 0.005 and P &lt; 0.01, respectively) and leukotriene D4 (2 nmol/kg; P &lt; 0.01) was significantly higher than in adult animals. U-46619 and leukotriene D4 produced significant extravasation of Evans Blue dye in both immature and adult animals. The order of potency to induce extravasated dye also did not change with age (leukotriene D4 &gt; U-46619). The amount of extravasation of Evans Blue dye after U-46619 (6 and 20 nmol/kg) and leukotriene D4 (0.6 and 2 nmol/kg) was significantly smaller in immature animals than adults at all airway levels.(ABSTRACT TRUNCATED AT 250 WORDS)}},
  author       = {{Arakawa, H and Lotvall, J and Kawikova, I and Tokuyama, K and Löfdahl, Claes-Göran and Skoogh, B E}},
  issn         = {{1879-0712}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{251--257}},
  publisher    = {{Elsevier}},
  series       = {{European Journal of Pharmacology}},
  title        = {{Effect of maturation on airway plasma exudation induced by eicosanoids in guinea pig}},
  volume       = {{259}},
  year         = {{1994}},
}