Advanced

Restricted variable region gene usage and possible rheumatoid factor relationship among human monoclonal antibodies specific for the AD-1 epitope on cytomegalovirus glycoprotein B

Ohlin, Mats LU ; Owman, Henrik LU ; Rioux, John D; Newkirk, Marianna M and Borrebaeck, Carl LU (1994) In Molecular Immunology 31(13). p.983-991
Abstract
The nucleotide sequences of the variable region genes encoding five different human, high affinity antibodies, specific for the major neutralization determinant (AD-1) expressed by human cytomegalovirus glycoprotein B (gp58/116), have been determined. Three of the five heavy chain variable regions belonged to the small VHV-family, although they combined with a diverse set of light chains (V kappa IIIb, V lambda II and V lambda III). The other two antibodies belonged to VH-families III and IV. One of the VHV-family genes most likely originated from a previously unreported germline gene or allele, since it carries a nine nucleotide insert in framework 1. In addition, V lambda-genes showed variable homology (77-95%) to known germline... (More)
The nucleotide sequences of the variable region genes encoding five different human, high affinity antibodies, specific for the major neutralization determinant (AD-1) expressed by human cytomegalovirus glycoprotein B (gp58/116), have been determined. Three of the five heavy chain variable regions belonged to the small VHV-family, although they combined with a diverse set of light chains (V kappa IIIb, V lambda II and V lambda III). The other two antibodies belonged to VH-families III and IV. One of the VHV-family genes most likely originated from a previously unreported germline gene or allele, since it carries a nine nucleotide insert in framework 1. In addition, V lambda-genes showed variable homology (77-95%) to known germline sequences, while V kappa-genes showed high homology (approximately 98%) with their proposed germline origin. Despite the close homology of the V kappa IIIb-gene used to express one of the antibodies with its corresponding germline gene, the protein did not strongly express some idiotypes associated with this light chain family. There is, thus, no direct relation between the expression of these crossreactive idiotypes and the use of even modestly mutated light chains belonging to this V kappa-family, which has been implicated in the development of anti-idiotypic networks possibly inducing autoantibodies, such as rheumatoid factors. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
cytomegalovirus, glycoprotein B, human monoclonal antibody, rheumatoid factor, variable region gene
in
Molecular Immunology
volume
31
issue
13
pages
983 - 991
publisher
Pergamon
external identifiers
  • pmid:7521934
  • scopus:0028074342
ISSN
1872-9142
DOI
10.1016/0161-5890(94)90093-0
language
English
LU publication?
yes
id
e5d4fab2-4a31-4b38-9bea-bac51bce447d (old id 1108329)
date added to LUP
2008-07-23 17:17:33
date last changed
2017-01-01 07:24:03
@article{e5d4fab2-4a31-4b38-9bea-bac51bce447d,
  abstract     = {The nucleotide sequences of the variable region genes encoding five different human, high affinity antibodies, specific for the major neutralization determinant (AD-1) expressed by human cytomegalovirus glycoprotein B (gp58/116), have been determined. Three of the five heavy chain variable regions belonged to the small VHV-family, although they combined with a diverse set of light chains (V kappa IIIb, V lambda II and V lambda III). The other two antibodies belonged to VH-families III and IV. One of the VHV-family genes most likely originated from a previously unreported germline gene or allele, since it carries a nine nucleotide insert in framework 1. In addition, V lambda-genes showed variable homology (77-95%) to known germline sequences, while V kappa-genes showed high homology (approximately 98%) with their proposed germline origin. Despite the close homology of the V kappa IIIb-gene used to express one of the antibodies with its corresponding germline gene, the protein did not strongly express some idiotypes associated with this light chain family. There is, thus, no direct relation between the expression of these crossreactive idiotypes and the use of even modestly mutated light chains belonging to this V kappa-family, which has been implicated in the development of anti-idiotypic networks possibly inducing autoantibodies, such as rheumatoid factors.},
  author       = {Ohlin, Mats and Owman, Henrik and Rioux, John D and Newkirk, Marianna M and Borrebaeck, Carl},
  issn         = {1872-9142},
  keyword      = {cytomegalovirus,glycoprotein B,human monoclonal antibody,rheumatoid factor,variable region gene},
  language     = {eng},
  number       = {13},
  pages        = {983--991},
  publisher    = {Pergamon},
  series       = {Molecular Immunology},
  title        = {Restricted variable region gene usage and possible rheumatoid factor relationship among human monoclonal antibodies specific for the AD-1 epitope on cytomegalovirus glycoprotein B},
  url          = {http://dx.doi.org/10.1016/0161-5890(94)90093-0},
  volume       = {31},
  year         = {1994},
}