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Serum cystatin C, determined by a rapid, automated particle-enhanced turbidimetric method, is a better marker than serum creatinine for glomerular filtration rate

Kyhse-Andersen, Jan; Schmidt, Camilla; Nordin, Gunnar; Andersson, Britta; Nilsson-Ehle, Peter LU ; Lindström, Veronica LU and Grubb, Anders LU (1994) In Clinical Chemistry 40(10). p.1921-1926
Abstract
We describe a fully automated particle-enhanced turbidimetric assay for cystatin C in undiluted serum and EDTA-plasma. The throughput is 90 samples per hour and urgent samples can be analyzed in 7 min. The assay range (0.4-14.1 mg/L) covers the concentration range in health and disease. The within- and between-run imprecision is 0.9% and 2.2%, respectively. Analytical recovery of additions of recombinant cystatin C averaged 98%. Rheumatoid factors (< or = 323,000 IU/L), bilirubin (< or = 150 mumol/L), hemoglobin (< or = 1.2 g/L), and triglycerides (< or = 8.5 mmol/L) do not interfere in the assay. In view of the superior (by ROC analysis) diagnostic accuracy of serum concentrations of cystatin C for reduced glomerular... (More)
We describe a fully automated particle-enhanced turbidimetric assay for cystatin C in undiluted serum and EDTA-plasma. The throughput is 90 samples per hour and urgent samples can be analyzed in 7 min. The assay range (0.4-14.1 mg/L) covers the concentration range in health and disease. The within- and between-run imprecision is 0.9% and 2.2%, respectively. Analytical recovery of additions of recombinant cystatin C averaged 98%. Rheumatoid factors (< or = 323,000 IU/L), bilirubin (< or = 150 mumol/L), hemoglobin (< or = 1.2 g/L), and triglycerides (< or = 8.5 mmol/L) do not interfere in the assay. In view of the superior (by ROC analysis) diagnostic accuracy of serum concentrations of cystatin C for reduced glomerular filtration rate (GFR) in comparison with creatinine, cystatin C seems an attractive alternative to creatinine for estimation of GFR. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Clinical Chemistry
volume
40
issue
10
pages
1921 - 1926
publisher
American Association for Clinical Chemistry
external identifiers
  • pmid:7923773
  • scopus:0028138577
ISSN
0009-9147
language
English
LU publication?
yes
id
ddfbcacb-54d6-4460-8646-4b4279a90631 (old id 1108363)
alternative location
http://www.clinchem.org/cgi/reprint/40/10/1921
date added to LUP
2008-07-23 17:46:05
date last changed
2017-07-02 03:36:38
@article{ddfbcacb-54d6-4460-8646-4b4279a90631,
  abstract     = {We describe a fully automated particle-enhanced turbidimetric assay for cystatin C in undiluted serum and EDTA-plasma. The throughput is 90 samples per hour and urgent samples can be analyzed in 7 min. The assay range (0.4-14.1 mg/L) covers the concentration range in health and disease. The within- and between-run imprecision is 0.9% and 2.2%, respectively. Analytical recovery of additions of recombinant cystatin C averaged 98%. Rheumatoid factors (&lt; or = 323,000 IU/L), bilirubin (&lt; or = 150 mumol/L), hemoglobin (&lt; or = 1.2 g/L), and triglycerides (&lt; or = 8.5 mmol/L) do not interfere in the assay. In view of the superior (by ROC analysis) diagnostic accuracy of serum concentrations of cystatin C for reduced glomerular filtration rate (GFR) in comparison with creatinine, cystatin C seems an attractive alternative to creatinine for estimation of GFR.},
  author       = {Kyhse-Andersen, Jan and Schmidt, Camilla and Nordin, Gunnar and Andersson, Britta and Nilsson-Ehle, Peter and Lindström, Veronica and Grubb, Anders},
  issn         = {0009-9147},
  language     = {eng},
  number       = {10},
  pages        = {1921--1926},
  publisher    = {American Association for Clinical Chemistry},
  series       = {Clinical Chemistry},
  title        = {Serum cystatin C, determined by a rapid, automated particle-enhanced turbidimetric method, is a better marker than serum creatinine for glomerular filtration rate},
  volume       = {40},
  year         = {1994},
}