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Oxidative stress decreases antioxidant enzyme activities in reaggregation cultures of rat brain cells

Aspberg, Anders LU and Tottmar, Olof (1994) In Free Radical Biology & Medicine 17(6). p.511-516
Abstract
The brain has been suggested to be especially sensitive to damage by reactive oxygen species. In this study, we examined the effects of hyperoxic conditions on the activities and mRNA levels of antioxidant enzymes in reaggregation cultures of rat forebrain cells. Cultures were exposed to 80% oxygen for 12-60 h starting on Days 17 and 33 in culture. Superoxide dismutase activities and mRNA levels were not affected by hyperoxia, whereas catalase activity was slightly decreased after 24 h in 80% oxygen at Day 17. Glutathione peroxidase activity was markedly decreased already after 12 h of hyperoxia, and decreased activities of glutathione reductase and glucose-6-phosphate dehydrogenase were also noted. The glutathione peroxidase mRNA levels... (More)
The brain has been suggested to be especially sensitive to damage by reactive oxygen species. In this study, we examined the effects of hyperoxic conditions on the activities and mRNA levels of antioxidant enzymes in reaggregation cultures of rat forebrain cells. Cultures were exposed to 80% oxygen for 12-60 h starting on Days 17 and 33 in culture. Superoxide dismutase activities and mRNA levels were not affected by hyperoxia, whereas catalase activity was slightly decreased after 24 h in 80% oxygen at Day 17. Glutathione peroxidase activity was markedly decreased already after 12 h of hyperoxia, and decreased activities of glutathione reductase and glucose-6-phosphate dehydrogenase were also noted. The glutathione peroxidase mRNA levels were increased in hyperoxic cultures at Day 17 but not at Day 33. These results suggest that the enzymatic defense mechanisms against reactive oxygen species in the brain are rather weak and deteriorate during oxidative stress but that a potential for compensatory upregulation exists at least during the first postnatal weeks. (Less)
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author
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Superoxide dismutase, Catalase, Glutathione peroxidase, Oxidative stress, Rat brain, Reaggregation cultures, Free radicals
in
Free Radical Biology & Medicine
volume
17
issue
6
pages
511 - 516
publisher
Elsevier
external identifiers
  • pmid:7867967
  • scopus:0028046390
ISSN
0891-5849
DOI
10.1016/0891-5849(94)90090-6
language
English
LU publication?
no
id
f7b3ad94-67ec-4079-a118-d1c69a989f06 (old id 1108637)
date added to LUP
2008-07-24 14:25:01
date last changed
2017-01-01 05:16:35
@article{f7b3ad94-67ec-4079-a118-d1c69a989f06,
  abstract     = {The brain has been suggested to be especially sensitive to damage by reactive oxygen species. In this study, we examined the effects of hyperoxic conditions on the activities and mRNA levels of antioxidant enzymes in reaggregation cultures of rat forebrain cells. Cultures were exposed to 80% oxygen for 12-60 h starting on Days 17 and 33 in culture. Superoxide dismutase activities and mRNA levels were not affected by hyperoxia, whereas catalase activity was slightly decreased after 24 h in 80% oxygen at Day 17. Glutathione peroxidase activity was markedly decreased already after 12 h of hyperoxia, and decreased activities of glutathione reductase and glucose-6-phosphate dehydrogenase were also noted. The glutathione peroxidase mRNA levels were increased in hyperoxic cultures at Day 17 but not at Day 33. These results suggest that the enzymatic defense mechanisms against reactive oxygen species in the brain are rather weak and deteriorate during oxidative stress but that a potential for compensatory upregulation exists at least during the first postnatal weeks.},
  author       = {Aspberg, Anders and Tottmar, Olof},
  issn         = {0891-5849},
  keyword      = {Superoxide dismutase,Catalase,Glutathione peroxidase,Oxidative stress,Rat brain,Reaggregation cultures,Free radicals},
  language     = {eng},
  number       = {6},
  pages        = {511--516},
  publisher    = {Elsevier},
  series       = {Free Radical Biology & Medicine},
  title        = {Oxidative stress decreases antioxidant enzyme activities in reaggregation cultures of rat brain cells},
  url          = {http://dx.doi.org/10.1016/0891-5849(94)90090-6},
  volume       = {17},
  year         = {1994},
}