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Ethanol-induced increase in catalase activity in reaggregation cultures of rat brain cells is due to increased oligodendrocyte differentiation

Aspberg, Anders LU and Tottmar, Olof (1994) In Alcoholism: Clinical and Experimental Research 18(3). p.620-624
Abstract
Recent studies have shown that ethanol exposure of reaggregation cultures of fetal rat brain cells causes an increased activity and amount of catalase, and also an increased activity of the oligodendrocyte marker enzyme 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNP). In the present study, reaggregation cultures were grown in the presence of 40 mM ethanol during 17 days, corresponding to a period in vivo from gestational day 17 to postnatal day 12. The activities of catalase, the peroxisomal marker enzyme acyl-coenzyme A oxidase and CNP were increased in ethanol-treated cultures. Immunofluorescence experiments confirmed that catalase mainly resided in oligodendrocytes. By immunoperoxidase staining for CNP followed by image analysis, it... (More)
Recent studies have shown that ethanol exposure of reaggregation cultures of fetal rat brain cells causes an increased activity and amount of catalase, and also an increased activity of the oligodendrocyte marker enzyme 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNP). In the present study, reaggregation cultures were grown in the presence of 40 mM ethanol during 17 days, corresponding to a period in vivo from gestational day 17 to postnatal day 12. The activities of catalase, the peroxisomal marker enzyme acyl-coenzyme A oxidase and CNP were increased in ethanol-treated cultures. Immunofluorescence experiments confirmed that catalase mainly resided in oligodendrocytes. By immunoperoxidase staining for CNP followed by image analysis, it was observed that the proportion of CNP-positive cells, as well as the intensity of staining, was increased in ethanol-treated cultures. Thus, the previously reported increase in catalase protein and activity in ethanol-treated cultures might be due to a stimulation, or earlier onset, of oligodendrocyte differentiation. (Less)
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author
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Catalase, Peroxisomes, Oligodendrocytes, Ethanol
in
Alcoholism: Clinical and Experimental Research
volume
18
issue
3
pages
620 - 624
publisher
Wiley-Blackwell
external identifiers
  • pmid:7943665
  • scopus:0028341545
ISSN
0145-6008
DOI
10.1111/j.1530-0277.1994.tb00920.x
language
English
LU publication?
no
id
22cd3b17-b575-4d2b-81e7-46434561487d (old id 1108639)
date added to LUP
2008-07-24 14:27:40
date last changed
2017-01-01 05:12:30
@article{22cd3b17-b575-4d2b-81e7-46434561487d,
  abstract     = {Recent studies have shown that ethanol exposure of reaggregation cultures of fetal rat brain cells causes an increased activity and amount of catalase, and also an increased activity of the oligodendrocyte marker enzyme 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNP). In the present study, reaggregation cultures were grown in the presence of 40 mM ethanol during 17 days, corresponding to a period in vivo from gestational day 17 to postnatal day 12. The activities of catalase, the peroxisomal marker enzyme acyl-coenzyme A oxidase and CNP were increased in ethanol-treated cultures. Immunofluorescence experiments confirmed that catalase mainly resided in oligodendrocytes. By immunoperoxidase staining for CNP followed by image analysis, it was observed that the proportion of CNP-positive cells, as well as the intensity of staining, was increased in ethanol-treated cultures. Thus, the previously reported increase in catalase protein and activity in ethanol-treated cultures might be due to a stimulation, or earlier onset, of oligodendrocyte differentiation.},
  author       = {Aspberg, Anders and Tottmar, Olof},
  issn         = {0145-6008},
  keyword      = {Catalase,Peroxisomes,Oligodendrocytes,Ethanol},
  language     = {eng},
  number       = {3},
  pages        = {620--624},
  publisher    = {Wiley-Blackwell},
  series       = {Alcoholism: Clinical and Experimental Research},
  title        = {Ethanol-induced increase in catalase activity in reaggregation cultures of rat brain cells is due to increased oligodendrocyte differentiation},
  url          = {http://dx.doi.org/10.1111/j.1530-0277.1994.tb00920.x},
  volume       = {18},
  year         = {1994},
}