Dual-label one-step immunoassay for simultaneous measurement of free and total prostate-specific antigen concentrations and ratios in serum
Mitrunen, Katja ; Pettersson, Kim ; Piironen, Timo ; Björk, Thomas LU ; Lilja, Hans LU
and Lövgren, Timo
(1995)
In Clinical Chemistry
41(8).
p.1115-1120
- Abstract
- We developed a simple one-step dual-label immunoassay for simultaneous measurement of the free, noncomplexed form of prostate-specific antigen (PSA) and total PSA. The assay is based on time-resolved fluorescence and includes a stable fluorescent chelate of Eu to label a monoclonal antibody (mAb) that detects only free PSA, whereas a second mAb labeled with a fluorescent chelate of Tb provides equimolar detection of both free PSA and PSA complexed to alpha 1-antichymotrypsin. A third mAb on a solid phase captures the free and complexed forms of PSA in an equimolar fashion. The simultaneous measurement of the free-to-total PSA ratio (F/T) with the one-step dual assay is not sensitive to variations in the sample volume. The discrimination... (More)
- We developed a simple one-step dual-label immunoassay for simultaneous measurement of the free, noncomplexed form of prostate-specific antigen (PSA) and total PSA. The assay is based on time-resolved fluorescence and includes a stable fluorescent chelate of Eu to label a monoclonal antibody (mAb) that detects only free PSA, whereas a second mAb labeled with a fluorescent chelate of Tb provides equimolar detection of both free PSA and PSA complexed to alpha 1-antichymotrypsin. A third mAb on a solid phase captures the free and complexed forms of PSA in an equimolar fashion. The simultaneous measurement of the free-to-total PSA ratio (F/T) with the one-step dual assay is not sensitive to variations in the sample volume. The discrimination between benign prostatic hyperplasia and prostate cancer patients, i.e., the area under the receiver-operating characteristic curve, increased from 0.64 (total PSA assay) to 0.78 and 0.81 when the F/T ratio was measured with single and dual assays, respectively. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1108991
- author
- Mitrunen, Katja
; Pettersson, Kim
; Piironen, Timo
; Björk, Thomas
LU
; Lilja, Hans
LU
and Lövgren, Timo
- organization
- publishing date
- 1995
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- prostate cancer/benign prostatic hyperplasia/timeresolved fluorescence immunoassay/monoclonal antibodies
- in
- Clinical Chemistry
- volume
- 41
- issue
- 8
- pages
- 1115 - 1120
- publisher
- American Association for Clinical Chemistry
- external identifiers
-
- pmid:7543033
- scopus:0029128039
- ISSN
- 0009-9147
- language
- English
- LU publication?
- yes
- id
- af975d6e-7c27-4c1d-b0ff-31b81e795ed4 (old id 1108991)
- alternative location
- http://www.clinchem.org/cgi/reprint/41/8/1115
- date added to LUP
- 2016-04-01 12:27:25
- date last changed
- 2021-09-19 04:22:19
@article{af975d6e-7c27-4c1d-b0ff-31b81e795ed4,
abstract = {{We developed a simple one-step dual-label immunoassay for simultaneous measurement of the free, noncomplexed form of prostate-specific antigen (PSA) and total PSA. The assay is based on time-resolved fluorescence and includes a stable fluorescent chelate of Eu to label a monoclonal antibody (mAb) that detects only free PSA, whereas a second mAb labeled with a fluorescent chelate of Tb provides equimolar detection of both free PSA and PSA complexed to alpha 1-antichymotrypsin. A third mAb on a solid phase captures the free and complexed forms of PSA in an equimolar fashion. The simultaneous measurement of the free-to-total PSA ratio (F/T) with the one-step dual assay is not sensitive to variations in the sample volume. The discrimination between benign prostatic hyperplasia and prostate cancer patients, i.e., the area under the receiver-operating characteristic curve, increased from 0.64 (total PSA assay) to 0.78 and 0.81 when the F/T ratio was measured with single and dual assays, respectively.}},
author = {{Mitrunen, Katja and Pettersson, Kim and Piironen, Timo and Björk, Thomas and Lilja, Hans and Lövgren, Timo}},
issn = {{0009-9147}},
keywords = {{prostate cancer/benign prostatic hyperplasia/timeresolved
fluorescence immunoassay/monoclonal antibodies}},
language = {{eng}},
number = {{8}},
pages = {{1115--1120}},
publisher = {{American Association for Clinical Chemistry}},
series = {{Clinical Chemistry}},
title = {{Dual-label one-step immunoassay for simultaneous measurement of free and total prostate-specific antigen concentrations and ratios in serum}},
url = {{http://www.clinchem.org/cgi/reprint/41/8/1115}},
volume = {{41}},
year = {{1995}},
}