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Dual-label one-step immunoassay for simultaneous measurement of free and total prostate-specific antigen concentrations and ratios in serum

Mitrunen, Katja; Pettersson, Kim; Piironen, Timo; Björk, Thomas LU ; Lilja, Hans LU and Lövgren, Timo (1995) In Clinical Chemistry 41(8). p.1115-1120
Abstract
We developed a simple one-step dual-label immunoassay for simultaneous measurement of the free, noncomplexed form of prostate-specific antigen (PSA) and total PSA. The assay is based on time-resolved fluorescence and includes a stable fluorescent chelate of Eu to label a monoclonal antibody (mAb) that detects only free PSA, whereas a second mAb labeled with a fluorescent chelate of Tb provides equimolar detection of both free PSA and PSA complexed to alpha 1-antichymotrypsin. A third mAb on a solid phase captures the free and complexed forms of PSA in an equimolar fashion. The simultaneous measurement of the free-to-total PSA ratio (F/T) with the one-step dual assay is not sensitive to variations in the sample volume. The discrimination... (More)
We developed a simple one-step dual-label immunoassay for simultaneous measurement of the free, noncomplexed form of prostate-specific antigen (PSA) and total PSA. The assay is based on time-resolved fluorescence and includes a stable fluorescent chelate of Eu to label a monoclonal antibody (mAb) that detects only free PSA, whereas a second mAb labeled with a fluorescent chelate of Tb provides equimolar detection of both free PSA and PSA complexed to alpha 1-antichymotrypsin. A third mAb on a solid phase captures the free and complexed forms of PSA in an equimolar fashion. The simultaneous measurement of the free-to-total PSA ratio (F/T) with the one-step dual assay is not sensitive to variations in the sample volume. The discrimination between benign prostatic hyperplasia and prostate cancer patients, i.e., the area under the receiver-operating characteristic curve, increased from 0.64 (total PSA assay) to 0.78 and 0.81 when the F/T ratio was measured with single and dual assays, respectively. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
prostate cancer/benign prostatic hyperplasia/timeresolved fluorescence immunoassay/monoclonal antibodies
in
Clinical Chemistry
volume
41
issue
8
pages
1115 - 1120
publisher
American Association for Clinical Chemistry
external identifiers
  • pmid:7543033
  • scopus:0029128039
ISSN
0009-9147
language
English
LU publication?
yes
id
af975d6e-7c27-4c1d-b0ff-31b81e795ed4 (old id 1108991)
alternative location
http://www.clinchem.org/cgi/reprint/41/8/1115
date added to LUP
2008-07-25 11:46:41
date last changed
2017-05-07 03:41:36
@article{af975d6e-7c27-4c1d-b0ff-31b81e795ed4,
  abstract     = {We developed a simple one-step dual-label immunoassay for simultaneous measurement of the free, noncomplexed form of prostate-specific antigen (PSA) and total PSA. The assay is based on time-resolved fluorescence and includes a stable fluorescent chelate of Eu to label a monoclonal antibody (mAb) that detects only free PSA, whereas a second mAb labeled with a fluorescent chelate of Tb provides equimolar detection of both free PSA and PSA complexed to alpha 1-antichymotrypsin. A third mAb on a solid phase captures the free and complexed forms of PSA in an equimolar fashion. The simultaneous measurement of the free-to-total PSA ratio (F/T) with the one-step dual assay is not sensitive to variations in the sample volume. The discrimination between benign prostatic hyperplasia and prostate cancer patients, i.e., the area under the receiver-operating characteristic curve, increased from 0.64 (total PSA assay) to 0.78 and 0.81 when the F/T ratio was measured with single and dual assays, respectively.},
  author       = {Mitrunen, Katja and Pettersson, Kim and Piironen, Timo and Björk, Thomas and Lilja, Hans and Lövgren, Timo},
  issn         = {0009-9147},
  keyword      = {prostate cancer/benign prostatic hyperplasia/timeresolved
fluorescence immunoassay/monoclonal antibodies},
  language     = {eng},
  number       = {8},
  pages        = {1115--1120},
  publisher    = {American Association for Clinical Chemistry},
  series       = {Clinical Chemistry},
  title        = {Dual-label one-step immunoassay for simultaneous measurement of free and total prostate-specific antigen concentrations and ratios in serum},
  volume       = {41},
  year         = {1995},
}